Molecular typing of canine parvovirus type 2 by VP2 gene sequencing and restriction fragment length polymorphism in affected dogs from Egypt
IntroductionCanine parvovirus-2 (CPV-2) is one of the most common infectious diseases in dogs characterized by severe gastroenteritis, vomiting, and bloody diarrhea. Little information is available about this topic in Egypt, particularly in the Delta region. This study reports the prevalence and mol...
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| Format: | Article |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1254060/full |
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| author | Asmaa Magouz Ismail El-Kon Enrique Raya-Álvarez Enas Khaled Noura Alkhalefa Alaa S. Alhegaili Manal F. El-khadragy Ahmad Agil Ehab Kotb Elmahallawy Ehab Kotb Elmahallawy |
| author_facet | Asmaa Magouz Ismail El-Kon Enrique Raya-Álvarez Enas Khaled Noura Alkhalefa Alaa S. Alhegaili Manal F. El-khadragy Ahmad Agil Ehab Kotb Elmahallawy Ehab Kotb Elmahallawy |
| author_sort | Asmaa Magouz |
| collection | DOAJ |
| description | IntroductionCanine parvovirus-2 (CPV-2) is one of the most common infectious diseases in dogs characterized by severe gastroenteritis, vomiting, and bloody diarrhea. Little information is available about this topic in Egypt, particularly in the Delta region. This study reports the prevalence and molecular analysis of CPV-2 variants collected from El-Gharbia and Kafrelsheikh governorates in the Delta of Egypt.MethodsIn this study, 320 rectal swabs were collected from infected domestic dogs from two districts in delta Egypt. The samples were investigated by rapid immunochromatographic test and polymerase chain reaction for detection the prevalence of CPV-2 variants. The genetic characterization was performed using restriction fragment length polymorphism (RFLP) analysis and partial VP2 gene sequence.Results and discussionThe viral antigen was detected in (264/320, 82.5%) of samples by IC test, while PCR was found more sensitive by detecting (272/320, 85%) positive samples. The RFLP technique using MboII restriction enzyme was successfully used for the differentiation of CPV-2c antigenic variants from CPV-2a/2b strains. Interestingly, the molecular and phylogenetic analysis revealed that both CPV-2a and CPV-2c are circulating in the study area. Deduced amino acid sequence analysis showed changes at residue (N426E) and residue (T440A).: Our results indicated that CPV-2 is prevalent among dogs in Egypt, and therefore further molecular and epidemiological studies of CPV-2 are warranted. |
| first_indexed | 2024-03-09T02:10:12Z |
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| id | doaj.art-d4640f97e59b40d8a1ce221b77b8e1a3 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-09T02:10:12Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d4640f97e59b40d8a1ce221b77b8e1a32023-12-07T13:51:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-12-011410.3389/fmicb.2023.12540601254060Molecular typing of canine parvovirus type 2 by VP2 gene sequencing and restriction fragment length polymorphism in affected dogs from EgyptAsmaa Magouz0Ismail El-Kon1Enrique Raya-Álvarez2Enas Khaled3Noura Alkhalefa4Alaa S. Alhegaili5Manal F. El-khadragy6Ahmad Agil7Ehab Kotb Elmahallawy8Ehab Kotb Elmahallawy9Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, EgyptDepartment of Gynaecology, Obstetrics and Artificial Insemination, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, EgyptRheumatology Department, Hospital Universitario San Cecilio, Granada, SpainDepartment of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, EgyptDepartment of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, EgyptDepartment of Medical Laboratory, College of Applied Medical Sciences, Prince Sattam bin Abdulaziz University, Alkharj, Saudi ArabiaDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Pharmacology, Biohealth Institute Granada (IBs Granada) and Neuroscience Institute, School of Medicine, University of Granada, Granada, SpainDepartamento de Sanidad Animal, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), Facultad de Veterinaria, Universidad de Córdoba, Córdoba, SpainDepartment of Zoonoses, Faculty of Veterinary Medicine, Sohag University, Sohag, EgyptIntroductionCanine parvovirus-2 (CPV-2) is one of the most common infectious diseases in dogs characterized by severe gastroenteritis, vomiting, and bloody diarrhea. Little information is available about this topic in Egypt, particularly in the Delta region. This study reports the prevalence and molecular analysis of CPV-2 variants collected from El-Gharbia and Kafrelsheikh governorates in the Delta of Egypt.MethodsIn this study, 320 rectal swabs were collected from infected domestic dogs from two districts in delta Egypt. The samples were investigated by rapid immunochromatographic test and polymerase chain reaction for detection the prevalence of CPV-2 variants. The genetic characterization was performed using restriction fragment length polymorphism (RFLP) analysis and partial VP2 gene sequence.Results and discussionThe viral antigen was detected in (264/320, 82.5%) of samples by IC test, while PCR was found more sensitive by detecting (272/320, 85%) positive samples. The RFLP technique using MboII restriction enzyme was successfully used for the differentiation of CPV-2c antigenic variants from CPV-2a/2b strains. Interestingly, the molecular and phylogenetic analysis revealed that both CPV-2a and CPV-2c are circulating in the study area. Deduced amino acid sequence analysis showed changes at residue (N426E) and residue (T440A).: Our results indicated that CPV-2 is prevalent among dogs in Egypt, and therefore further molecular and epidemiological studies of CPV-2 are warranted.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1254060/fullcanine parvovirusPCRRFLPVP2 geneEgypt |
| spellingShingle | Asmaa Magouz Ismail El-Kon Enrique Raya-Álvarez Enas Khaled Noura Alkhalefa Alaa S. Alhegaili Manal F. El-khadragy Ahmad Agil Ehab Kotb Elmahallawy Ehab Kotb Elmahallawy Molecular typing of canine parvovirus type 2 by VP2 gene sequencing and restriction fragment length polymorphism in affected dogs from Egypt Frontiers in Microbiology canine parvovirus PCR RFLP VP2 gene Egypt |
| title | Molecular typing of canine parvovirus type 2 by VP2 gene sequencing and restriction fragment length polymorphism in affected dogs from Egypt |
| title_full | Molecular typing of canine parvovirus type 2 by VP2 gene sequencing and restriction fragment length polymorphism in affected dogs from Egypt |
| title_fullStr | Molecular typing of canine parvovirus type 2 by VP2 gene sequencing and restriction fragment length polymorphism in affected dogs from Egypt |
| title_full_unstemmed | Molecular typing of canine parvovirus type 2 by VP2 gene sequencing and restriction fragment length polymorphism in affected dogs from Egypt |
| title_short | Molecular typing of canine parvovirus type 2 by VP2 gene sequencing and restriction fragment length polymorphism in affected dogs from Egypt |
| title_sort | molecular typing of canine parvovirus type 2 by vp2 gene sequencing and restriction fragment length polymorphism in affected dogs from egypt |
| topic | canine parvovirus PCR RFLP VP2 gene Egypt |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1254060/full |
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