Catechol-O-methyltransferase (COMT) protein expression and activity after dopaminergic and noradrenergic lesions of the rat brain.
The occurrence of catechol-O-methyltransferase (COMT) in presynaptic neurons remains controversial. This study utilized dopaminergic and noradrenergic toxins to assess the presence of COMT in the presynaptic neurons originating from the substantia nigra, ventral tegmental area or locus coeruleus. De...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23613844/pdf/?tool=EBI |
| _version_ | 1818458937309003776 |
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| author | Nadia Schendzielorz Juha-Pekka Oinas Timo T Myöhänen Ilkka Reenilä Atso Raasmaja Pekka T Männistö |
| author_facet | Nadia Schendzielorz Juha-Pekka Oinas Timo T Myöhänen Ilkka Reenilä Atso Raasmaja Pekka T Männistö |
| author_sort | Nadia Schendzielorz |
| collection | DOAJ |
| description | The occurrence of catechol-O-methyltransferase (COMT) in presynaptic neurons remains controversial. This study utilized dopaminergic and noradrenergic toxins to assess the presence of COMT in the presynaptic neurons originating from the substantia nigra, ventral tegmental area or locus coeruleus. Destruction of dopaminergic and noradrenergic neurons was assessed by measuring the dopamine and noradrenaline content in the projection areas of these neurons. Additionally, COMT protein expression and activity were examined in several projection areas to determine whether there are any changes in COMT values. Colocalization studies were done to identify COMT-containing postsynaptic neurons. Despite successful lesioning of dopaminergic and noradrenergic neurons, no changes in COMT protein expression or activity could be noted. These results strongly suggest that COMT is not present in presynaptic dopaminergic and noradrenergic neurons. There was a high colocalization of COMT with the GABAergic marker of short neurons both in the striatum and cortex but only a weak, if any, with the cholinergic marker in the cortex. |
| first_indexed | 2024-12-14T23:06:23Z |
| format | Article |
| id | doaj.art-d46a53daed10414a835828b620908505 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T23:06:23Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d46a53daed10414a835828b6209085052022-12-21T22:44:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6139210.1371/journal.pone.0061392Catechol-O-methyltransferase (COMT) protein expression and activity after dopaminergic and noradrenergic lesions of the rat brain.Nadia SchendzielorzJuha-Pekka OinasTimo T MyöhänenIlkka ReeniläAtso RaasmajaPekka T MännistöThe occurrence of catechol-O-methyltransferase (COMT) in presynaptic neurons remains controversial. This study utilized dopaminergic and noradrenergic toxins to assess the presence of COMT in the presynaptic neurons originating from the substantia nigra, ventral tegmental area or locus coeruleus. Destruction of dopaminergic and noradrenergic neurons was assessed by measuring the dopamine and noradrenaline content in the projection areas of these neurons. Additionally, COMT protein expression and activity were examined in several projection areas to determine whether there are any changes in COMT values. Colocalization studies were done to identify COMT-containing postsynaptic neurons. Despite successful lesioning of dopaminergic and noradrenergic neurons, no changes in COMT protein expression or activity could be noted. These results strongly suggest that COMT is not present in presynaptic dopaminergic and noradrenergic neurons. There was a high colocalization of COMT with the GABAergic marker of short neurons both in the striatum and cortex but only a weak, if any, with the cholinergic marker in the cortex.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23613844/pdf/?tool=EBI |
| spellingShingle | Nadia Schendzielorz Juha-Pekka Oinas Timo T Myöhänen Ilkka Reenilä Atso Raasmaja Pekka T Männistö Catechol-O-methyltransferase (COMT) protein expression and activity after dopaminergic and noradrenergic lesions of the rat brain. PLoS ONE |
| title | Catechol-O-methyltransferase (COMT) protein expression and activity after dopaminergic and noradrenergic lesions of the rat brain. |
| title_full | Catechol-O-methyltransferase (COMT) protein expression and activity after dopaminergic and noradrenergic lesions of the rat brain. |
| title_fullStr | Catechol-O-methyltransferase (COMT) protein expression and activity after dopaminergic and noradrenergic lesions of the rat brain. |
| title_full_unstemmed | Catechol-O-methyltransferase (COMT) protein expression and activity after dopaminergic and noradrenergic lesions of the rat brain. |
| title_short | Catechol-O-methyltransferase (COMT) protein expression and activity after dopaminergic and noradrenergic lesions of the rat brain. |
| title_sort | catechol o methyltransferase comt protein expression and activity after dopaminergic and noradrenergic lesions of the rat brain |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23613844/pdf/?tool=EBI |
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