Quantum dots reveal heterogeneous membrane diffusivity and dynamic surface density polarization of dopamine transporter.
The presynaptic dopamine transporter mediates rapid reuptake of synaptic dopamine. Although cell surface DAT trafficking recently emerged as an important component of DAT regulation, it has not been systematically investigated. Here, we apply our single quantum dot (Qdot) tracking approach to monito...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0225339 |
| _version_ | 1818725893609095168 |
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| author | Oleg Kovtun Ian D Tomlinson Riley S Ferguson Sandra J Rosenthal |
| author_facet | Oleg Kovtun Ian D Tomlinson Riley S Ferguson Sandra J Rosenthal |
| author_sort | Oleg Kovtun |
| collection | DOAJ |
| description | The presynaptic dopamine transporter mediates rapid reuptake of synaptic dopamine. Although cell surface DAT trafficking recently emerged as an important component of DAT regulation, it has not been systematically investigated. Here, we apply our single quantum dot (Qdot) tracking approach to monitor DAT plasma membrane dynamics in several heterologous expression cell hosts with nanometer localization accuracy. We demonstrate that Qdot-tagged DAT proteins exhibited highly heterogeneous membrane diffusivity dependent on the local membrane topography. We also show that Qdot-tagged DATs were localized away from the flat membrane regions and were dynamically retained in the membrane protrusions and cell edges for the duration of imaging. Single quantum dot tracking of wildtype DAT and its conformation-defective coding variants (R60A and W63A) revealed a significantly accelerated rate of dysfunctional DAT membrane diffusion. We believe our results warrant an in-depth investigation as to whether compromised membrane dynamics is a common feature of brain disorder-derived DAT mutants. |
| first_indexed | 2024-12-17T21:49:33Z |
| format | Article |
| id | doaj.art-d46b5ee14c7e450c87c09ac87571de2e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T21:49:33Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d46b5ee14c7e450c87c09ac87571de2e2022-12-21T21:31:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011411e022533910.1371/journal.pone.0225339Quantum dots reveal heterogeneous membrane diffusivity and dynamic surface density polarization of dopamine transporter.Oleg KovtunIan D TomlinsonRiley S FergusonSandra J RosenthalThe presynaptic dopamine transporter mediates rapid reuptake of synaptic dopamine. Although cell surface DAT trafficking recently emerged as an important component of DAT regulation, it has not been systematically investigated. Here, we apply our single quantum dot (Qdot) tracking approach to monitor DAT plasma membrane dynamics in several heterologous expression cell hosts with nanometer localization accuracy. We demonstrate that Qdot-tagged DAT proteins exhibited highly heterogeneous membrane diffusivity dependent on the local membrane topography. We also show that Qdot-tagged DATs were localized away from the flat membrane regions and were dynamically retained in the membrane protrusions and cell edges for the duration of imaging. Single quantum dot tracking of wildtype DAT and its conformation-defective coding variants (R60A and W63A) revealed a significantly accelerated rate of dysfunctional DAT membrane diffusion. We believe our results warrant an in-depth investigation as to whether compromised membrane dynamics is a common feature of brain disorder-derived DAT mutants.https://doi.org/10.1371/journal.pone.0225339 |
| spellingShingle | Oleg Kovtun Ian D Tomlinson Riley S Ferguson Sandra J Rosenthal Quantum dots reveal heterogeneous membrane diffusivity and dynamic surface density polarization of dopamine transporter. PLoS ONE |
| title | Quantum dots reveal heterogeneous membrane diffusivity and dynamic surface density polarization of dopamine transporter. |
| title_full | Quantum dots reveal heterogeneous membrane diffusivity and dynamic surface density polarization of dopamine transporter. |
| title_fullStr | Quantum dots reveal heterogeneous membrane diffusivity and dynamic surface density polarization of dopamine transporter. |
| title_full_unstemmed | Quantum dots reveal heterogeneous membrane diffusivity and dynamic surface density polarization of dopamine transporter. |
| title_short | Quantum dots reveal heterogeneous membrane diffusivity and dynamic surface density polarization of dopamine transporter. |
| title_sort | quantum dots reveal heterogeneous membrane diffusivity and dynamic surface density polarization of dopamine transporter |
| url | https://doi.org/10.1371/journal.pone.0225339 |
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