Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019
ABSTRACT: Objectives: This study assessed the incidence and resistance of Mycoplasma pneumoniae (MP) in children in Qingdao, China, in 2019. Methods: We detected MP infection in 78 pharyngeal swabs from children with pneumonia by qPCR. The RepMP4 element in the P1 adhesin gene, domain V of the 23S...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716521002186 |
| _version_ | 1818950411456872448 |
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| author | Fa-Chun Jiang Rui-Fang Wang Ping Chen Li-Yan Dong Xia Wang Qin Song Yi-Qiu Wan Qin-Qin Song Juan Song Yan-Hai Wang Zhi-Qiang Xia Dong Xia Jun Han |
| author_facet | Fa-Chun Jiang Rui-Fang Wang Ping Chen Li-Yan Dong Xia Wang Qin Song Yi-Qiu Wan Qin-Qin Song Juan Song Yan-Hai Wang Zhi-Qiang Xia Dong Xia Jun Han |
| author_sort | Fa-Chun Jiang |
| collection | DOAJ |
| description | ABSTRACT: Objectives: This study assessed the incidence and resistance of Mycoplasma pneumoniae (MP) in children in Qingdao, China, in 2019. Methods: We detected MP infection in 78 pharyngeal swabs from children with pneumonia by qPCR. The RepMP4 element in the P1 adhesin gene, domain V of the 23S rRNA gene, and the L4/L22 ribosomal proteins were amplified by nested PCR. Evolutionary analysis was conducted based on the P1 gene sequence. Resistance mutations in domain V of the 23S rRNA gene and L4/L22 ribosomal proteins were analysed. Results: The incidence of MP infection in children with pneumonia was 59.0% (46/78). The mean duration of MP infection was longer than that of non-MP infection. According to P1 gene sequencing of 21 samples, 12 (57.1%) were type 1 and 9 (42.9%) were type 2. Drug resistance mutations A2063G in domain V of 23S rRNA gene and T508C in L22 were identified from all sequenced MP. However, mutations at positions 2064 and 2617 were not found in this study. C162A mutation appeared in most type 2 samples. A430G mutation appeared in one type 1 sample and in several type 2 samples. T279C mutation in L22 was mostly found in type 2 samples. Conclusion: The incidence of MP infection was 59.0% in children with pneumonia in Qingdao in 2019. Type 1 MP infection was slightly more common than type 2, indicating that the genotype of MP is gradually shifting from type 1 to type 2. Macrolide resistance mutation A2063G could be detected in all sequenced MP. |
| first_indexed | 2024-12-20T09:18:10Z |
| format | Article |
| id | doaj.art-d46e5a5868824dd98ced62b99580c94e |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-20T09:18:10Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-d46e5a5868824dd98ced62b99580c94e2022-12-21T19:45:21ZengElsevierJournal of Global Antimicrobial Resistance2213-71652021-12-0127273278Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019Fa-Chun Jiang0Rui-Fang Wang1Ping Chen2Li-Yan Dong3Xia Wang4Qin Song5Yi-Qiu Wan6Qin-Qin Song7Juan Song8Yan-Hai Wang9Zhi-Qiang Xia10Dong Xia11Jun Han12Municipal Centre of Disease Control and Prevention of Qingdao, Qingdao Institute of Prevention Medicine, Qingdao 266033, Shandong, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Bengbu Medical College, Bengbu 233030, Anhui, ChinaMunicipal Centre of Disease Control and Prevention of Qingdao, Qingdao Institute of Prevention Medicine, Qingdao 266033, Shandong, ChinaDistrict Center of Disease Control and Prevention of Shibei, Qingdao 266000, Shandong, ChinaDistrict Center of Disease Control and Prevention of Chengyang, Qingdao 266041, Shandong, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Medical College of Anhui University of Science and Technology, Huainan 232001, Anhui, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Corresponding author.ABSTRACT: Objectives: This study assessed the incidence and resistance of Mycoplasma pneumoniae (MP) in children in Qingdao, China, in 2019. Methods: We detected MP infection in 78 pharyngeal swabs from children with pneumonia by qPCR. The RepMP4 element in the P1 adhesin gene, domain V of the 23S rRNA gene, and the L4/L22 ribosomal proteins were amplified by nested PCR. Evolutionary analysis was conducted based on the P1 gene sequence. Resistance mutations in domain V of the 23S rRNA gene and L4/L22 ribosomal proteins were analysed. Results: The incidence of MP infection in children with pneumonia was 59.0% (46/78). The mean duration of MP infection was longer than that of non-MP infection. According to P1 gene sequencing of 21 samples, 12 (57.1%) were type 1 and 9 (42.9%) were type 2. Drug resistance mutations A2063G in domain V of 23S rRNA gene and T508C in L22 were identified from all sequenced MP. However, mutations at positions 2064 and 2617 were not found in this study. C162A mutation appeared in most type 2 samples. A430G mutation appeared in one type 1 sample and in several type 2 samples. T279C mutation in L22 was mostly found in type 2 samples. Conclusion: The incidence of MP infection was 59.0% in children with pneumonia in Qingdao in 2019. Type 1 MP infection was slightly more common than type 2, indicating that the genotype of MP is gradually shifting from type 1 to type 2. Macrolide resistance mutation A2063G could be detected in all sequenced MP.http://www.sciencedirect.com/science/article/pii/S2213716521002186Mycoplasma pneumoniaePneumoniaChildrenMacrolide resistanceDrug resistance gene |
| spellingShingle | Fa-Chun Jiang Rui-Fang Wang Ping Chen Li-Yan Dong Xia Wang Qin Song Yi-Qiu Wan Qin-Qin Song Juan Song Yan-Hai Wang Zhi-Qiang Xia Dong Xia Jun Han Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019 Journal of Global Antimicrobial Resistance Mycoplasma pneumoniae Pneumonia Children Macrolide resistance Drug resistance gene |
| title | Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019 |
| title_full | Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019 |
| title_fullStr | Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019 |
| title_full_unstemmed | Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019 |
| title_short | Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019 |
| title_sort | genotype and mutation patterns of macrolide resistance genes of mycoplasma pneumoniae from children with pneumonia in qingdao china in 2019 |
| topic | Mycoplasma pneumoniae Pneumonia Children Macrolide resistance Drug resistance gene |
| url | http://www.sciencedirect.com/science/article/pii/S2213716521002186 |
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