DsFoxO knockout affects development and fecundity of Drosophila suzukii
Forkhead box O (FoxO), a key transcription factor in many species, participates in numerous physiological and pathological processes of organisms through a variety of signaling pathways. In the present study, we established DsFoxO knockout (DsFoxO-KO) strain using CRISPR/Cas9, and the influence on d...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1290732/full |
| _version_ | 1797522035821248512 |
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| author | Shan Zhao Shan Zhao Shan Zhao Ruijuan Wang Ruijuan Wang Ruijuan Wang Yan Liu Yan Liu Yan Liu Long Su Long Su Long Su Xiaoyan Dai Xiaoyan Dai Xiaoyan Dai Dongyun Qin Dongyun Qin Hao Chen Hao Chen Hao Chen Zhenjuan Yin Zhenjuan Yin Li Zheng Li Zheng Li Zheng Yifan Zhai Yifan Zhai Yifan Zhai |
| author_facet | Shan Zhao Shan Zhao Shan Zhao Ruijuan Wang Ruijuan Wang Ruijuan Wang Yan Liu Yan Liu Yan Liu Long Su Long Su Long Su Xiaoyan Dai Xiaoyan Dai Xiaoyan Dai Dongyun Qin Dongyun Qin Hao Chen Hao Chen Hao Chen Zhenjuan Yin Zhenjuan Yin Li Zheng Li Zheng Li Zheng Yifan Zhai Yifan Zhai Yifan Zhai |
| author_sort | Shan Zhao |
| collection | DOAJ |
| description | Forkhead box O (FoxO), a key transcription factor in many species, participates in numerous physiological and pathological processes of organisms through a variety of signaling pathways. In the present study, we established DsFoxO knockout (DsFoxO-KO) strain using CRISPR/Cas9, and the influence on development and fecundity of mutant strain were evaluated. To clarify the corresponding mechanism, a transcriptome analysis was conducted subsequently. The results showed that the survival rates of the DsFoxO-KO strain in larval, pupal, and adult stages were all significantly lower than those of control. The duration of the pupal stage was similar between the two strains; however, durations of egg, larva, adult preoviposition period (APOP), and total APOP (TPOP) in the DsFoxO-KO strain were all significantly longer compared to those of the control strain. The fecundity of the DsFoxO-KO strain was 20.31 eggs/female, which was significantly lower than that of the control strain (430.47 eggs/female). With the transcriptome analysis, 612 differentially expressed genes (DEGs) were identified. Following COG and GO analyses, we found that most of the DEGs were associated with the metabolic process. According to the KEGG database, the mTOR signaling, MAPK signaling, Wnt signaling, and Toll and Imd signaling pathways; insect hormone biosynthesis; autophagy; and apoptosis were altered in the DsFoxO-KO strain. These results demonstrated that knockout of DsFoxO in D. suzukii significantly influenced its development and fecundity, while transcriptome analysis provided insights to explore the corresponding molecular mechanism. These findings highlighted the critical role of FoxO in D. suzukii and might contribute to the development of novel management strategies for these flies in the future. |
| first_indexed | 2024-03-10T08:20:57Z |
| format | Article |
| id | doaj.art-d470a52856c24eba9ad9afa6ca7504de |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-03-10T08:20:57Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-d470a52856c24eba9ad9afa6ca7504de2023-11-22T09:58:14ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-11-011410.3389/fphys.2023.12907321290732DsFoxO knockout affects development and fecundity of Drosophila suzukiiShan Zhao0Shan Zhao1Shan Zhao2Ruijuan Wang3Ruijuan Wang4Ruijuan Wang5Yan Liu6Yan Liu7Yan Liu8Long Su9Long Su10Long Su11Xiaoyan Dai12Xiaoyan Dai13Xiaoyan Dai14Dongyun Qin15Dongyun Qin16Hao Chen17Hao Chen18Hao Chen19Zhenjuan Yin20Zhenjuan Yin21Li Zheng22Li Zheng23Li Zheng24Yifan Zhai25Yifan Zhai26Yifan Zhai27Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaKey Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, Jinan, ChinaChina Ministry of Agriculture and Rural Affairs (MARA) ‐ Centre for Agriculture and Bioscience International (CABI) Joint Laboratory for Bio‐Safety Shandong Sub‐Center, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaKey Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, Jinan, ChinaChina Ministry of Agriculture and Rural Affairs (MARA) ‐ Centre for Agriculture and Bioscience International (CABI) Joint Laboratory for Bio‐Safety Shandong Sub‐Center, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaKey Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, Jinan, ChinaChina Ministry of Agriculture and Rural Affairs (MARA) ‐ Centre for Agriculture and Bioscience International (CABI) Joint Laboratory for Bio‐Safety Shandong Sub‐Center, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaKey Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, Jinan, ChinaChina Ministry of Agriculture and Rural Affairs (MARA) ‐ Centre for Agriculture and Bioscience International (CABI) Joint Laboratory for Bio‐Safety Shandong Sub‐Center, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaKey Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, Jinan, ChinaChina Ministry of Agriculture and Rural Affairs (MARA) ‐ Centre for Agriculture and Bioscience International (CABI) Joint Laboratory for Bio‐Safety Shandong Sub‐Center, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaKey Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaKey Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, Jinan, ChinaChina Ministry of Agriculture and Rural Affairs (MARA) ‐ Centre for Agriculture and Bioscience International (CABI) Joint Laboratory for Bio‐Safety Shandong Sub‐Center, Jinan, ChinaKey Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, Jinan, ChinaCollege of Agriculture, Guizhou University, Guiyang, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaKey Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, Jinan, ChinaChina Ministry of Agriculture and Rural Affairs (MARA) ‐ Centre for Agriculture and Bioscience International (CABI) Joint Laboratory for Bio‐Safety Shandong Sub‐Center, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaKey Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, Jinan, ChinaChina Ministry of Agriculture and Rural Affairs (MARA) ‐ Centre for Agriculture and Bioscience International (CABI) Joint Laboratory for Bio‐Safety Shandong Sub‐Center, Jinan, ChinaForkhead box O (FoxO), a key transcription factor in many species, participates in numerous physiological and pathological processes of organisms through a variety of signaling pathways. In the present study, we established DsFoxO knockout (DsFoxO-KO) strain using CRISPR/Cas9, and the influence on development and fecundity of mutant strain were evaluated. To clarify the corresponding mechanism, a transcriptome analysis was conducted subsequently. The results showed that the survival rates of the DsFoxO-KO strain in larval, pupal, and adult stages were all significantly lower than those of control. The duration of the pupal stage was similar between the two strains; however, durations of egg, larva, adult preoviposition period (APOP), and total APOP (TPOP) in the DsFoxO-KO strain were all significantly longer compared to those of the control strain. The fecundity of the DsFoxO-KO strain was 20.31 eggs/female, which was significantly lower than that of the control strain (430.47 eggs/female). With the transcriptome analysis, 612 differentially expressed genes (DEGs) were identified. Following COG and GO analyses, we found that most of the DEGs were associated with the metabolic process. According to the KEGG database, the mTOR signaling, MAPK signaling, Wnt signaling, and Toll and Imd signaling pathways; insect hormone biosynthesis; autophagy; and apoptosis were altered in the DsFoxO-KO strain. These results demonstrated that knockout of DsFoxO in D. suzukii significantly influenced its development and fecundity, while transcriptome analysis provided insights to explore the corresponding molecular mechanism. These findings highlighted the critical role of FoxO in D. suzukii and might contribute to the development of novel management strategies for these flies in the future.https://www.frontiersin.org/articles/10.3389/fphys.2023.1290732/fullD. suzukiiDsFoxOdevelopmentfecunditytranscriptome analysis |
| spellingShingle | Shan Zhao Shan Zhao Shan Zhao Ruijuan Wang Ruijuan Wang Ruijuan Wang Yan Liu Yan Liu Yan Liu Long Su Long Su Long Su Xiaoyan Dai Xiaoyan Dai Xiaoyan Dai Dongyun Qin Dongyun Qin Hao Chen Hao Chen Hao Chen Zhenjuan Yin Zhenjuan Yin Li Zheng Li Zheng Li Zheng Yifan Zhai Yifan Zhai Yifan Zhai DsFoxO knockout affects development and fecundity of Drosophila suzukii Frontiers in Physiology D. suzukii DsFoxO development fecundity transcriptome analysis |
| title | DsFoxO knockout affects development and fecundity of Drosophila suzukii |
| title_full | DsFoxO knockout affects development and fecundity of Drosophila suzukii |
| title_fullStr | DsFoxO knockout affects development and fecundity of Drosophila suzukii |
| title_full_unstemmed | DsFoxO knockout affects development and fecundity of Drosophila suzukii |
| title_short | DsFoxO knockout affects development and fecundity of Drosophila suzukii |
| title_sort | dsfoxo knockout affects development and fecundity of drosophila suzukii |
| topic | D. suzukii DsFoxO development fecundity transcriptome analysis |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1290732/full |
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