A Versatile Peroxidase from the Fungus <i>Bjerkandera adusta</i> Confers Abiotic Stress Tolerance in Transgenic Tobacco Plants
White-rot fungi are efficient lignin degraders due to the secretion of lignin peroxidase, manganese peroxidase, laccase, and versatile peroxidase (VP) on decayed wood. The VP is a high-redox-potential enzyme and could be used to detoxify reactive oxygen species (ROS), which accumulate in plants duri...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/5/859 |
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| author | Nancy Sofia Hernández-Bueno Ramón Suárez-Rodríguez Edgar Balcázar-López Jorge Luis Folch-Mallol José Augusto Ramírez-Trujillo Gabriel Iturriaga |
| author_facet | Nancy Sofia Hernández-Bueno Ramón Suárez-Rodríguez Edgar Balcázar-López Jorge Luis Folch-Mallol José Augusto Ramírez-Trujillo Gabriel Iturriaga |
| author_sort | Nancy Sofia Hernández-Bueno |
| collection | DOAJ |
| description | White-rot fungi are efficient lignin degraders due to the secretion of lignin peroxidase, manganese peroxidase, laccase, and versatile peroxidase (VP) on decayed wood. The VP is a high-redox-potential enzyme and could be used to detoxify reactive oxygen species (ROS), which accumulate in plants during biotic and abiotic stresses. We cloned the <i>VP</i> gene and expressed it via the <i>Agrobacterium</i> transformation procedure in transgenic tobacco plants to assay their tolerance to different abiotic stress conditions. Thirty independent T<sub>2</sub> transgenic VP lines overexpressing the fungal <i>Bjerkandera adusta</i><i>VP</i> gene were selected on kanamycin. The VP22, VP24, and VP27 lines showed significant manganese peroxidase (MnP) activity. The highest was VP22, which showed 10.87-fold more manganese peroxidase activity than the wild-type plants and led to a 34% increase in plant height and 28% more biomass. The VP22, VP24, and VP27 lines showed enhanced tolerance to drought, 200 mM NaCl, and 400 mM sorbitol. Also, these transgenics displayed significant tolerance to methyl viologen, an active oxygen-generating compound. The present data indicate that overproducing the <i>VP</i> gene in plants increases significantly their biomass and the abiotic stress tolerance. The VP enzyme is an effective biotechnological tool to protect organisms against ROS. In transgenic tobacco plants, it improves drought, salt, and oxidative stress tolerance. Thus, the <i>VP</i> gene represents a great potential for obtaining stress-tolerant crops. |
| first_indexed | 2024-03-10T12:01:16Z |
| format | Article |
| id | doaj.art-d4713dd2f7794465b281b9397ab66a0a |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T12:01:16Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-d4713dd2f7794465b281b9397ab66a0a2023-11-21T16:56:33ZengMDPI AGPlants2223-77472021-04-0110585910.3390/plants10050859A Versatile Peroxidase from the Fungus <i>Bjerkandera adusta</i> Confers Abiotic Stress Tolerance in Transgenic Tobacco PlantsNancy Sofia Hernández-Bueno0Ramón Suárez-Rodríguez1Edgar Balcázar-López2Jorge Luis Folch-Mallol3José Augusto Ramírez-Trujillo4Gabriel Iturriaga5Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca 62209, Morelos, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca 62209, Morelos, MexicoDepartamento de Farmacobiología, Centro Universitario de Ciencias Exactas e Ingeniería, Universidad de Guadalajara, Blvd. M. García Barragán # 1451, Guadalajara 44430, Jalisco C.P., MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca 62209, Morelos, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca 62209, Morelos, MexicoDivisión de Estudios de Posgrado e Investigación, Tecnológico Nacional de México/I.T. Roque, Km. 8 Carretera Celaya-Juventino Rosas, Roque, Celaya 38110, Guanajuato, MexicoWhite-rot fungi are efficient lignin degraders due to the secretion of lignin peroxidase, manganese peroxidase, laccase, and versatile peroxidase (VP) on decayed wood. The VP is a high-redox-potential enzyme and could be used to detoxify reactive oxygen species (ROS), which accumulate in plants during biotic and abiotic stresses. We cloned the <i>VP</i> gene and expressed it via the <i>Agrobacterium</i> transformation procedure in transgenic tobacco plants to assay their tolerance to different abiotic stress conditions. Thirty independent T<sub>2</sub> transgenic VP lines overexpressing the fungal <i>Bjerkandera adusta</i><i>VP</i> gene were selected on kanamycin. The VP22, VP24, and VP27 lines showed significant manganese peroxidase (MnP) activity. The highest was VP22, which showed 10.87-fold more manganese peroxidase activity than the wild-type plants and led to a 34% increase in plant height and 28% more biomass. The VP22, VP24, and VP27 lines showed enhanced tolerance to drought, 200 mM NaCl, and 400 mM sorbitol. Also, these transgenics displayed significant tolerance to methyl viologen, an active oxygen-generating compound. The present data indicate that overproducing the <i>VP</i> gene in plants increases significantly their biomass and the abiotic stress tolerance. The VP enzyme is an effective biotechnological tool to protect organisms against ROS. In transgenic tobacco plants, it improves drought, salt, and oxidative stress tolerance. Thus, the <i>VP</i> gene represents a great potential for obtaining stress-tolerant crops.https://www.mdpi.com/2223-7747/10/5/859abiotic stress<i>Bjerkandera adusta</i>transgenic tobaccoversatile peroxidase |
| spellingShingle | Nancy Sofia Hernández-Bueno Ramón Suárez-Rodríguez Edgar Balcázar-López Jorge Luis Folch-Mallol José Augusto Ramírez-Trujillo Gabriel Iturriaga A Versatile Peroxidase from the Fungus <i>Bjerkandera adusta</i> Confers Abiotic Stress Tolerance in Transgenic Tobacco Plants Plants abiotic stress <i>Bjerkandera adusta</i> transgenic tobacco versatile peroxidase |
| title | A Versatile Peroxidase from the Fungus <i>Bjerkandera adusta</i> Confers Abiotic Stress Tolerance in Transgenic Tobacco Plants |
| title_full | A Versatile Peroxidase from the Fungus <i>Bjerkandera adusta</i> Confers Abiotic Stress Tolerance in Transgenic Tobacco Plants |
| title_fullStr | A Versatile Peroxidase from the Fungus <i>Bjerkandera adusta</i> Confers Abiotic Stress Tolerance in Transgenic Tobacco Plants |
| title_full_unstemmed | A Versatile Peroxidase from the Fungus <i>Bjerkandera adusta</i> Confers Abiotic Stress Tolerance in Transgenic Tobacco Plants |
| title_short | A Versatile Peroxidase from the Fungus <i>Bjerkandera adusta</i> Confers Abiotic Stress Tolerance in Transgenic Tobacco Plants |
| title_sort | versatile peroxidase from the fungus i bjerkandera adusta i confers abiotic stress tolerance in transgenic tobacco plants |
| topic | abiotic stress <i>Bjerkandera adusta</i> transgenic tobacco versatile peroxidase |
| url | https://www.mdpi.com/2223-7747/10/5/859 |
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