MicroRNA 3113-5p is a novel marker for early cardiac ischemia/reperfusion injury
Abstract Background Ischemia/reperfusion (I/R) injury of heart is one of the major causes of acute cardiac injury, which may result in worsening or even loss of heart function. With novel microRNAs being evolutionarily discovered, numbers of them remained functionally unknown. We aimed to discover n...
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | Diagnostic Pathology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13000-019-0894-1 |
| _version_ | 1819029147970699264 |
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| author | Yuanyuan Chen Xing Ye Fengping Yan |
| author_facet | Yuanyuan Chen Xing Ye Fengping Yan |
| author_sort | Yuanyuan Chen |
| collection | DOAJ |
| description | Abstract Background Ischemia/reperfusion (I/R) injury of heart is one of the major causes of acute cardiac injury, which may result in worsening or even loss of heart function. With novel microRNAs being evolutionarily discovered, numbers of them remained functionally unknown. We aimed to discover novel microRNAs with therapeutic or diagnostic potential in the setting of early cardiac I/R injury. Methods Cardiac electrical activity, biochemical detection and histopathology analysis were performed to reveal early changes of cardiac I/R injury. A microRNA array was performed to screen differential microRNAs in the mouse model of cardiac I/R injury. The differentially expressed microRNAs were validated in cardiac tissues and in serum samples. Results The abnormality in electrocardiogram and increases in serum cTnI levels suggested the successful establishment of cardiac I/R injury in mice. A total of 1882 microRNAs were identified, of which 11 were significantly down-regulated and 41 were significantly up-regulated at 3 h post reperfusion. microRNA 223-3p and microRNA 3113-5p were among the mostly altered microRNAs and were validated to be up-regulated within the early hours of I/R injury in heart tissues. In the circulating system, cTnI, a sensitive marker of cardiac injury, or microRNA 223-3p only increased within the first 6 h post I/R injury. However, microRNA 3113-5p stably increased in the serum, keeping an increase of 2.5-fold throughout the 24 h. In the human serum samples, microRNA 3113-5p was detected to be significantly upregulated as soon as 3 h after I/R stimuli and kept significantly higher levels within the 48 h. Conclusion This is the first study that reported the functional roles of microRNA 3113-5p in cardiovascular system. Our data suggested that cardiac microRNA 3113-5p might be a useful target for therapeutic purposes and circulating microRNA 3113-5p might serve as a stable marker for early diagnosis of cardiac I/R injury. |
| first_indexed | 2024-12-21T06:09:39Z |
| format | Article |
| id | doaj.art-d472e37930e0481fbf644374f3c1ada2 |
| institution | Directory Open Access Journal |
| issn | 1746-1596 |
| language | English |
| last_indexed | 2024-12-21T06:09:39Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic Pathology |
| spelling | doaj.art-d472e37930e0481fbf644374f3c1ada22022-12-21T19:13:34ZengBMCDiagnostic Pathology1746-15962019-10-0114111110.1186/s13000-019-0894-1MicroRNA 3113-5p is a novel marker for early cardiac ischemia/reperfusion injuryYuanyuan Chen0Xing Ye1Fengping Yan2Department of Forensic Medicine, School of Basic Medical Sciences, Gannan Medical CollegeDepartment of Forensic Medicine, School of Basic Medical Sciences, Gannan Medical CollegeDepartment of Forensic Medicine, School of Basic Medical Sciences, Gannan Medical CollegeAbstract Background Ischemia/reperfusion (I/R) injury of heart is one of the major causes of acute cardiac injury, which may result in worsening or even loss of heart function. With novel microRNAs being evolutionarily discovered, numbers of them remained functionally unknown. We aimed to discover novel microRNAs with therapeutic or diagnostic potential in the setting of early cardiac I/R injury. Methods Cardiac electrical activity, biochemical detection and histopathology analysis were performed to reveal early changes of cardiac I/R injury. A microRNA array was performed to screen differential microRNAs in the mouse model of cardiac I/R injury. The differentially expressed microRNAs were validated in cardiac tissues and in serum samples. Results The abnormality in electrocardiogram and increases in serum cTnI levels suggested the successful establishment of cardiac I/R injury in mice. A total of 1882 microRNAs were identified, of which 11 were significantly down-regulated and 41 were significantly up-regulated at 3 h post reperfusion. microRNA 223-3p and microRNA 3113-5p were among the mostly altered microRNAs and were validated to be up-regulated within the early hours of I/R injury in heart tissues. In the circulating system, cTnI, a sensitive marker of cardiac injury, or microRNA 223-3p only increased within the first 6 h post I/R injury. However, microRNA 3113-5p stably increased in the serum, keeping an increase of 2.5-fold throughout the 24 h. In the human serum samples, microRNA 3113-5p was detected to be significantly upregulated as soon as 3 h after I/R stimuli and kept significantly higher levels within the 48 h. Conclusion This is the first study that reported the functional roles of microRNA 3113-5p in cardiovascular system. Our data suggested that cardiac microRNA 3113-5p might be a useful target for therapeutic purposes and circulating microRNA 3113-5p might serve as a stable marker for early diagnosis of cardiac I/R injury.http://link.springer.com/article/10.1186/s13000-019-0894-1Ischemia/reperfusion injuryHeartCardiac biomarkersmicroRNA 3113-5p |
| spellingShingle | Yuanyuan Chen Xing Ye Fengping Yan MicroRNA 3113-5p is a novel marker for early cardiac ischemia/reperfusion injury Diagnostic Pathology Ischemia/reperfusion injury Heart Cardiac biomarkers microRNA 3113-5p |
| title | MicroRNA 3113-5p is a novel marker for early cardiac ischemia/reperfusion injury |
| title_full | MicroRNA 3113-5p is a novel marker for early cardiac ischemia/reperfusion injury |
| title_fullStr | MicroRNA 3113-5p is a novel marker for early cardiac ischemia/reperfusion injury |
| title_full_unstemmed | MicroRNA 3113-5p is a novel marker for early cardiac ischemia/reperfusion injury |
| title_short | MicroRNA 3113-5p is a novel marker for early cardiac ischemia/reperfusion injury |
| title_sort | microrna 3113 5p is a novel marker for early cardiac ischemia reperfusion injury |
| topic | Ischemia/reperfusion injury Heart Cardiac biomarkers microRNA 3113-5p |
| url | http://link.springer.com/article/10.1186/s13000-019-0894-1 |
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