Recycling of Chinese herb residues by endophytic and probiotic fungus Aspergillus cristatus CB10002 for the production of medicinal valuable anthraquinones
Abstract Background The global prevalence of traditional Chinese medicine stimulates the prosperous development of herb medicines, but the annual generation of massive herb residues becomes big issues about environmental pollution and waste of resources. Microbes play important roles in the circulat...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-06-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12934-019-1150-9 |
| _version_ | 1818257814847488000 |
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| author | Wenping Kong Chengshuang Huang Jie Shi Yu Li Xinxin Jiang Quwen Duan Yong Huang Yanwen Duan Xiangcheng Zhu |
| author_facet | Wenping Kong Chengshuang Huang Jie Shi Yu Li Xinxin Jiang Quwen Duan Yong Huang Yanwen Duan Xiangcheng Zhu |
| author_sort | Wenping Kong |
| collection | DOAJ |
| description | Abstract Background The global prevalence of traditional Chinese medicine stimulates the prosperous development of herb medicines, but the annual generation of massive herb residues becomes big issues about environmental pollution and waste of resources. Microbes play important roles in the circulation of substances in nature, and endophytes represent an underexplored microbial resource possessing the unique symbiotic relationship with plants, not only for discovery of secondary metabolites, but also for potential green recycling of herb residues. Results The recycling capacities of several endophytic strains were respectively evaluated via solid state fermentation with herb residues of commercial Huazhenghuisheng oral-liquid (HOL). Among them, Aspergillus cristatus CB10002, a probiotic fungus isolated from Chinese Fu-brick tea, was competent to recycle HOL residues for the production of medicinal valuable anthraquinones, in which four of them, especially citreorosein with significant anti-obesity activity, were first discovered in A. cristatus. Subsequent quantitative analysis showed that about 2.0 mg/g citreorosein and 7.5 mg/g total anthraquinones could be obtained after 35-day fermentation, which was very competitive and economically beneficial. Further nutritional comparisons also revealed that the recycling process indeed ameliorated the nutrients of HOL residues, and thus proposed a possibility to directly dispose the final leftovers as a compost organic fertilizer. Conclusions The endophytic and probiotic fungus A. cristatus CB10002 isolated from Chinese Fu-brick tea was screened out to effectively reutilize HOL residues for the production of nine medicinal valuable anthraquinones, whose biosynthesis may be regulated by the induction of HOL residues. The competitive yields of these anthraquinones, as well as the certain composting properties of final leftovers, have made the microbial recycling of HOL residues economically beneficial. Our work demonstrated a promising applied potential of A. cristatus in reutilization of herb residues, and provided a practical strategy for sustainable and value-added microbial recycling of herb residues. |
| first_indexed | 2024-12-12T17:49:38Z |
| format | Article |
| id | doaj.art-d473b64452a143b7aaa6cf553636b05b |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-12T17:49:38Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-d473b64452a143b7aaa6cf553636b05b2022-12-22T00:16:51ZengBMCMicrobial Cell Factories1475-28592019-06-0118111110.1186/s12934-019-1150-9Recycling of Chinese herb residues by endophytic and probiotic fungus Aspergillus cristatus CB10002 for the production of medicinal valuable anthraquinonesWenping Kong0Chengshuang Huang1Jie Shi2Yu Li3Xinxin Jiang4Quwen Duan5Yong Huang6Yanwen Duan7Xiangcheng Zhu8Xiangya International Academy of Translational Medicine, Central South UniversityXiangya International Academy of Translational Medicine, Central South UniversityXiangya International Academy of Translational Medicine, Central South UniversityXiangya International Academy of Translational Medicine, Central South UniversityHayaocihang Pharmaceutical Co. LtdHayaocihang Pharmaceutical Co. LtdXiangya International Academy of Translational Medicine, Central South UniversityXiangya International Academy of Translational Medicine, Central South UniversityXiangya International Academy of Translational Medicine, Central South UniversityAbstract Background The global prevalence of traditional Chinese medicine stimulates the prosperous development of herb medicines, but the annual generation of massive herb residues becomes big issues about environmental pollution and waste of resources. Microbes play important roles in the circulation of substances in nature, and endophytes represent an underexplored microbial resource possessing the unique symbiotic relationship with plants, not only for discovery of secondary metabolites, but also for potential green recycling of herb residues. Results The recycling capacities of several endophytic strains were respectively evaluated via solid state fermentation with herb residues of commercial Huazhenghuisheng oral-liquid (HOL). Among them, Aspergillus cristatus CB10002, a probiotic fungus isolated from Chinese Fu-brick tea, was competent to recycle HOL residues for the production of medicinal valuable anthraquinones, in which four of them, especially citreorosein with significant anti-obesity activity, were first discovered in A. cristatus. Subsequent quantitative analysis showed that about 2.0 mg/g citreorosein and 7.5 mg/g total anthraquinones could be obtained after 35-day fermentation, which was very competitive and economically beneficial. Further nutritional comparisons also revealed that the recycling process indeed ameliorated the nutrients of HOL residues, and thus proposed a possibility to directly dispose the final leftovers as a compost organic fertilizer. Conclusions The endophytic and probiotic fungus A. cristatus CB10002 isolated from Chinese Fu-brick tea was screened out to effectively reutilize HOL residues for the production of nine medicinal valuable anthraquinones, whose biosynthesis may be regulated by the induction of HOL residues. The competitive yields of these anthraquinones, as well as the certain composting properties of final leftovers, have made the microbial recycling of HOL residues economically beneficial. Our work demonstrated a promising applied potential of A. cristatus in reutilization of herb residues, and provided a practical strategy for sustainable and value-added microbial recycling of herb residues.http://link.springer.com/article/10.1186/s12934-019-1150-9Herb residuesMicrobial recyclingAspergillus cristatusSolid state fermentationMedicinal valuable anthraquinones |
| spellingShingle | Wenping Kong Chengshuang Huang Jie Shi Yu Li Xinxin Jiang Quwen Duan Yong Huang Yanwen Duan Xiangcheng Zhu Recycling of Chinese herb residues by endophytic and probiotic fungus Aspergillus cristatus CB10002 for the production of medicinal valuable anthraquinones Microbial Cell Factories Herb residues Microbial recycling Aspergillus cristatus Solid state fermentation Medicinal valuable anthraquinones |
| title | Recycling of Chinese herb residues by endophytic and probiotic fungus Aspergillus cristatus CB10002 for the production of medicinal valuable anthraquinones |
| title_full | Recycling of Chinese herb residues by endophytic and probiotic fungus Aspergillus cristatus CB10002 for the production of medicinal valuable anthraquinones |
| title_fullStr | Recycling of Chinese herb residues by endophytic and probiotic fungus Aspergillus cristatus CB10002 for the production of medicinal valuable anthraquinones |
| title_full_unstemmed | Recycling of Chinese herb residues by endophytic and probiotic fungus Aspergillus cristatus CB10002 for the production of medicinal valuable anthraquinones |
| title_short | Recycling of Chinese herb residues by endophytic and probiotic fungus Aspergillus cristatus CB10002 for the production of medicinal valuable anthraquinones |
| title_sort | recycling of chinese herb residues by endophytic and probiotic fungus aspergillus cristatus cb10002 for the production of medicinal valuable anthraquinones |
| topic | Herb residues Microbial recycling Aspergillus cristatus Solid state fermentation Medicinal valuable anthraquinones |
| url | http://link.springer.com/article/10.1186/s12934-019-1150-9 |
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