Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus
Porcine sapelovirus (PSV) is an important emerging pathogen associated with a wide variety of diseases in swine, including acute diarrhoea, respiratory distress, skin lesions, severe neurological disorders, and reproductive failure. Although PSV is widespread, serological assays for field-based epid...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/2/349 |
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| author | Yassein M. Ibrahim Wenli Zhang Gebremeskel Mamu Werid He Zhang Yawen Feng Yu Pan Lin Zhang Changwen Li Huan Lin Hongyan Chen Yue Wang |
| author_facet | Yassein M. Ibrahim Wenli Zhang Gebremeskel Mamu Werid He Zhang Yawen Feng Yu Pan Lin Zhang Changwen Li Huan Lin Hongyan Chen Yue Wang |
| author_sort | Yassein M. Ibrahim |
| collection | DOAJ |
| description | Porcine sapelovirus (PSV) is an important emerging pathogen associated with a wide variety of diseases in swine, including acute diarrhoea, respiratory distress, skin lesions, severe neurological disorders, and reproductive failure. Although PSV is widespread, serological assays for field-based epidemiological studies are not yet available. Here, four PSV strains were recovered from diarrheic piglets, and electron microscopy revealed virus particles with a diameter of ~32 nm. Analysis of the entire genome sequence revealed that the genomes of PSV isolates ranged 7569–7572 nucleotides in length. Phylogenetic analysis showed that the isolated viruses were classified together with strains from China. Additionally, monoclonal antibodies for the recombinant PSV-VP1 protein were developed to specifically detect PSV infection in cells, and we demonstrated that isolated PSVs could only replicate in cells of porcine origin. Using recombinant PSV-VP1 protein as the coating antigen, we developed an indirect ELISA for the first time for the detection of PSV antibodies in serum. A total of 516 swine serum samples were tested, and PSV positive rate was 79.3%. The virus isolates, monoclonal antibodies and indirect ELISA developed would be useful for further understanding the pathophysiology of PSV, developing new diagnostic assays, and investigating the epidemiology of the PSV. |
| first_indexed | 2024-03-09T20:51:59Z |
| format | Article |
| id | doaj.art-d4756acdb53c426aa31947c623ba25d3 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T20:51:59Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-d4756acdb53c426aa31947c623ba25d32023-11-23T22:31:22ZengMDPI AGViruses1999-49152022-02-0114234910.3390/v14020349Isolation, Characterization, and Molecular Detection of Porcine SapelovirusYassein M. Ibrahim0Wenli Zhang1Gebremeskel Mamu Werid2He Zhang3Yawen Feng4Yu Pan5Lin Zhang6Changwen Li7Huan Lin8Hongyan Chen9Yue Wang10Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaHeilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaHeilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaHeilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaLaboratory of Inspection and Testing, Hebei Provincial Station of Veterinary Drug and Feed, Shijiazhuang 050000, ChinaHeilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaHeilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaHeilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaHeilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaHeilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaHeilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaPorcine sapelovirus (PSV) is an important emerging pathogen associated with a wide variety of diseases in swine, including acute diarrhoea, respiratory distress, skin lesions, severe neurological disorders, and reproductive failure. Although PSV is widespread, serological assays for field-based epidemiological studies are not yet available. Here, four PSV strains were recovered from diarrheic piglets, and electron microscopy revealed virus particles with a diameter of ~32 nm. Analysis of the entire genome sequence revealed that the genomes of PSV isolates ranged 7569–7572 nucleotides in length. Phylogenetic analysis showed that the isolated viruses were classified together with strains from China. Additionally, monoclonal antibodies for the recombinant PSV-VP1 protein were developed to specifically detect PSV infection in cells, and we demonstrated that isolated PSVs could only replicate in cells of porcine origin. Using recombinant PSV-VP1 protein as the coating antigen, we developed an indirect ELISA for the first time for the detection of PSV antibodies in serum. A total of 516 swine serum samples were tested, and PSV positive rate was 79.3%. The virus isolates, monoclonal antibodies and indirect ELISA developed would be useful for further understanding the pathophysiology of PSV, developing new diagnostic assays, and investigating the epidemiology of the PSV.https://www.mdpi.com/1999-4915/14/2/349porcine sapelovirusisolationcharacterizationprevalencemonoclonal antibodiesELISA |
| spellingShingle | Yassein M. Ibrahim Wenli Zhang Gebremeskel Mamu Werid He Zhang Yawen Feng Yu Pan Lin Zhang Changwen Li Huan Lin Hongyan Chen Yue Wang Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus Viruses porcine sapelovirus isolation characterization prevalence monoclonal antibodies ELISA |
| title | Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus |
| title_full | Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus |
| title_fullStr | Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus |
| title_full_unstemmed | Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus |
| title_short | Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus |
| title_sort | isolation characterization and molecular detection of porcine sapelovirus |
| topic | porcine sapelovirus isolation characterization prevalence monoclonal antibodies ELISA |
| url | https://www.mdpi.com/1999-4915/14/2/349 |
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