Coi1-Dependent Signaling Pathway Is Not Required for Mi-1—Mediated Potato Aphid Resistance
ABSTRACT Tomato (Solanum lycopersicum) has a unique resistance gene, Mi-1, that confers resistance to animals from distinct taxa, nematodes, and piercing and sucking insects. Mi-1 encodes a protein with a nucleotide-binding site and leucine-rich repeat motifs. Early in the potato aphid (Macrosiphum...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2007-03-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-3-0276 |
| _version_ | 1818647116640157696 |
|---|---|
| author | Kishor K. Bhattarai Qi-Guang Xie Daniel Pourshalimi Ted Younglove Isgouhi Kaloshian |
| author_facet | Kishor K. Bhattarai Qi-Guang Xie Daniel Pourshalimi Ted Younglove Isgouhi Kaloshian |
| author_sort | Kishor K. Bhattarai |
| collection | DOAJ |
| description | ABSTRACT Tomato (Solanum lycopersicum) has a unique resistance gene, Mi-1, that confers resistance to animals from distinct taxa, nematodes, and piercing and sucking insects. Mi-1 encodes a protein with a nucleotide-binding site and leucine-rich repeat motifs. Early in the potato aphid (Macrosiphum euphorbiae)—tomato interactions, aphid feeding induces the expression of the jasmonic acid (JA)-regulated proteinase inhibitor genes, Pin1 and Pin2. The jai1-1 (jasmonic acid insensitive 1) tomato mutant, which is impaired in JA perception, was used to gain additional insight into the JA signaling pathway and its role in the Mi-1—mediated aphid resistance. The jai1-1 mutant has a deletion in the Coi1 gene that encodes a putative F-box protein. In this study, aphid colonization, survival, and fecundity were compared on wild-type tomato and jai1-1 mutant. In choice assays, the jai1-1 mutant showed higher colonization by potato aphids compared with wild-type tomato. In contrast, no-choice assays showed no difference in potato aphid survival or fecundity between jai1-1 and the wild-type parent. Plants homozygous for Mi-1 and for the jai1 mutation were not compromised in resistance to potato aphids, using either choice or no-choice assays. In addition, the accumulation of JA-regulated Pin1 transcripts after aphid feeding was Coi1 dependent. Taken together, these data indicate that, although potato aphids activate Coi1-dependent defense response in tomato, this response is not required for Mi-1—mediated resistance to aphids. |
| first_indexed | 2024-12-17T00:57:25Z |
| format | Article |
| id | doaj.art-d4789ab709de4422930e11a514eab853 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T00:57:25Z |
| publishDate | 2007-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-d4789ab709de4422930e11a514eab8532022-12-21T22:09:34ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062007-03-0120327628210.1094/MPMI-20-3-0276Coi1-Dependent Signaling Pathway Is Not Required for Mi-1—Mediated Potato Aphid ResistanceKishor K. BhattaraiQi-Guang XieDaniel PourshalimiTed YoungloveIsgouhi KaloshianABSTRACT Tomato (Solanum lycopersicum) has a unique resistance gene, Mi-1, that confers resistance to animals from distinct taxa, nematodes, and piercing and sucking insects. Mi-1 encodes a protein with a nucleotide-binding site and leucine-rich repeat motifs. Early in the potato aphid (Macrosiphum euphorbiae)—tomato interactions, aphid feeding induces the expression of the jasmonic acid (JA)-regulated proteinase inhibitor genes, Pin1 and Pin2. The jai1-1 (jasmonic acid insensitive 1) tomato mutant, which is impaired in JA perception, was used to gain additional insight into the JA signaling pathway and its role in the Mi-1—mediated aphid resistance. The jai1-1 mutant has a deletion in the Coi1 gene that encodes a putative F-box protein. In this study, aphid colonization, survival, and fecundity were compared on wild-type tomato and jai1-1 mutant. In choice assays, the jai1-1 mutant showed higher colonization by potato aphids compared with wild-type tomato. In contrast, no-choice assays showed no difference in potato aphid survival or fecundity between jai1-1 and the wild-type parent. Plants homozygous for Mi-1 and for the jai1 mutation were not compromised in resistance to potato aphids, using either choice or no-choice assays. In addition, the accumulation of JA-regulated Pin1 transcripts after aphid feeding was Coi1 dependent. Taken together, these data indicate that, although potato aphids activate Coi1-dependent defense response in tomato, this response is not required for Mi-1—mediated resistance to aphids.https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-3-0276 |
| spellingShingle | Kishor K. Bhattarai Qi-Guang Xie Daniel Pourshalimi Ted Younglove Isgouhi Kaloshian Coi1-Dependent Signaling Pathway Is Not Required for Mi-1—Mediated Potato Aphid Resistance Molecular Plant-Microbe Interactions |
| title | Coi1-Dependent Signaling Pathway Is Not Required for Mi-1—Mediated Potato Aphid Resistance |
| title_full | Coi1-Dependent Signaling Pathway Is Not Required for Mi-1—Mediated Potato Aphid Resistance |
| title_fullStr | Coi1-Dependent Signaling Pathway Is Not Required for Mi-1—Mediated Potato Aphid Resistance |
| title_full_unstemmed | Coi1-Dependent Signaling Pathway Is Not Required for Mi-1—Mediated Potato Aphid Resistance |
| title_short | Coi1-Dependent Signaling Pathway Is Not Required for Mi-1—Mediated Potato Aphid Resistance |
| title_sort | coi1 dependent signaling pathway is not required for mi 1 mediated potato aphid resistance |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-3-0276 |
| work_keys_str_mv | AT kishorkbhattarai coi1dependentsignalingpathwayisnotrequiredformi1mediatedpotatoaphidresistance AT qiguangxie coi1dependentsignalingpathwayisnotrequiredformi1mediatedpotatoaphidresistance AT danielpourshalimi coi1dependentsignalingpathwayisnotrequiredformi1mediatedpotatoaphidresistance AT tedyounglove coi1dependentsignalingpathwayisnotrequiredformi1mediatedpotatoaphidresistance AT isgouhikaloshian coi1dependentsignalingpathwayisnotrequiredformi1mediatedpotatoaphidresistance |