Whole slide image with image analysis of atypical bile duct brushing: Quantitative features predictive of malignancy
Background: Whole slide images (WSIs) involve digitally capturing glass slides for microscopic computer-based viewing and these are amenable to quantitative image analysis. Bile duct (BD) brushing can show morphologic features that are categorized as indeterminate for malignancy. The study aims to e...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-01-01
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| Series: | Journal of Pathology Informatics |
| Subjects: | |
| Online Access: | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2015;volume=6;issue=1;spage=47;epage=47;aulast=Collins |
| _version_ | 1818264275134709760 |
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| author | Brian T Collins Cody R Weimholt |
| author_facet | Brian T Collins Cody R Weimholt |
| author_sort | Brian T Collins |
| collection | DOAJ |
| description | Background: Whole slide images (WSIs) involve digitally capturing glass slides for microscopic computer-based viewing and these are amenable to quantitative image analysis. Bile duct (BD) brushing can show morphologic features that are categorized as indeterminate for malignancy. The study aims to evaluate quantitative morphologic features of atypical categories of BD brushing by WSI analysis for the identification of criteria predictive of malignancy. Materials and Methods: Over a 3-year period, BD brush specimens with indeterminate diagnostic categorization (atypical to suspicious) were subjected to WSI analysis. Ten well-visualized groups with morphologic atypical features were selected per case and had the quantitative analysis performed for group area, individual nuclear area, the number of nuclei per group, N: C ratio and nuclear size differential. Results: There were 28 cases identified with 17 atypical and 11 suspicious. The average nuclear area was 63.7 µm2 for atypical and 80.1 µm2 for suspicious (+difference 16.4 µm2; P = 0.002). The nuclear size differential was 69.7 µm2 for atypical and 88.4 µm2 for suspicious (+difference 18.8 µm2; P = 0.009). An average nuclear area >70 µm2 had a 3.2 risk ratio for suspicious categorization. Conclusion: The quantitative criteria findings as measured by image analysis on WSI showed that cases categorized as suspicious had more nuclear size pleomorphism (+18.8 µm2) and larger nuclei (+16.4 µm2) than those categorized as atypical. WSI with morphologic image analysis can demonstrate quantitative statistically significant differences between atypical and suspicious BD brushings and provide objective criteria that support the diagnosis of carcinoma. |
| first_indexed | 2024-12-12T19:32:19Z |
| format | Article |
| id | doaj.art-d47c07ccfa494fb98b1382bce9db6376 |
| institution | Directory Open Access Journal |
| issn | 2153-3539 |
| language | English |
| last_indexed | 2024-12-12T19:32:19Z |
| publishDate | 2015-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pathology Informatics |
| spelling | doaj.art-d47c07ccfa494fb98b1382bce9db63762022-12-22T00:14:24ZengElsevierJournal of Pathology Informatics2153-35392015-01-0161474710.4103/2153-3539.163986Whole slide image with image analysis of atypical bile duct brushing: Quantitative features predictive of malignancyBrian T CollinsCody R WeimholtBackground: Whole slide images (WSIs) involve digitally capturing glass slides for microscopic computer-based viewing and these are amenable to quantitative image analysis. Bile duct (BD) brushing can show morphologic features that are categorized as indeterminate for malignancy. The study aims to evaluate quantitative morphologic features of atypical categories of BD brushing by WSI analysis for the identification of criteria predictive of malignancy. Materials and Methods: Over a 3-year period, BD brush specimens with indeterminate diagnostic categorization (atypical to suspicious) were subjected to WSI analysis. Ten well-visualized groups with morphologic atypical features were selected per case and had the quantitative analysis performed for group area, individual nuclear area, the number of nuclei per group, N: C ratio and nuclear size differential. Results: There were 28 cases identified with 17 atypical and 11 suspicious. The average nuclear area was 63.7 µm2 for atypical and 80.1 µm2 for suspicious (+difference 16.4 µm2; P = 0.002). The nuclear size differential was 69.7 µm2 for atypical and 88.4 µm2 for suspicious (+difference 18.8 µm2; P = 0.009). An average nuclear area >70 µm2 had a 3.2 risk ratio for suspicious categorization. Conclusion: The quantitative criteria findings as measured by image analysis on WSI showed that cases categorized as suspicious had more nuclear size pleomorphism (+18.8 µm2) and larger nuclei (+16.4 µm2) than those categorized as atypical. WSI with morphologic image analysis can demonstrate quantitative statistically significant differences between atypical and suspicious BD brushings and provide objective criteria that support the diagnosis of carcinoma.http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2015;volume=6;issue=1;spage=47;epage=47;aulast=CollinsAdenocarcinoma, bile duct, cytology image analysis, whole slide image |
| spellingShingle | Brian T Collins Cody R Weimholt Whole slide image with image analysis of atypical bile duct brushing: Quantitative features predictive of malignancy Journal of Pathology Informatics Adenocarcinoma, bile duct, cytology image analysis, whole slide image |
| title | Whole slide image with image analysis of atypical bile duct brushing: Quantitative features predictive of malignancy |
| title_full | Whole slide image with image analysis of atypical bile duct brushing: Quantitative features predictive of malignancy |
| title_fullStr | Whole slide image with image analysis of atypical bile duct brushing: Quantitative features predictive of malignancy |
| title_full_unstemmed | Whole slide image with image analysis of atypical bile duct brushing: Quantitative features predictive of malignancy |
| title_short | Whole slide image with image analysis of atypical bile duct brushing: Quantitative features predictive of malignancy |
| title_sort | whole slide image with image analysis of atypical bile duct brushing quantitative features predictive of malignancy |
| topic | Adenocarcinoma, bile duct, cytology image analysis, whole slide image |
| url | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2015;volume=6;issue=1;spage=47;epage=47;aulast=Collins |
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