Correlation between Pathogenic Determinants Associated with Clinically Isolated Non-Typhoidal <i>Salmonella</i>
Non-typhoidal and Typhoidal <i>Salmonella</i> are bacterial pathogens source of worldwide and major disease burden. Virulent determinants of specific serovars belonging to non-typhoidal <i>Salmonella</i> have been extensively studied in different models, yet the pathogenesis...
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MDPI AG
2021-01-01
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| Online Access: | https://www.mdpi.com/2076-0817/10/1/74 |
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| author | Boimpoundi Eunice Flavie Ouali Tsyr-Huei Chiou Jenn-Wei Chen I-Chu Lin Ching-Chuan Liu Yu-Chung Chiang Tzong-Shiann Ho Hao-Ven Wang |
| author_facet | Boimpoundi Eunice Flavie Ouali Tsyr-Huei Chiou Jenn-Wei Chen I-Chu Lin Ching-Chuan Liu Yu-Chung Chiang Tzong-Shiann Ho Hao-Ven Wang |
| author_sort | Boimpoundi Eunice Flavie Ouali |
| collection | DOAJ |
| description | Non-typhoidal and Typhoidal <i>Salmonella</i> are bacterial pathogens source of worldwide and major disease burden. Virulent determinants of specific serovars belonging to non-typhoidal <i>Salmonella</i> have been extensively studied in different models, yet the pathogenesis of this group of bacteria and the development of clinical symptoms globally remains underexplored. Herein, we implemented microbiological and molecular procedures to investigate isolate virulence traits and molecular diversity, likely in association with disease severity. Our results show that selected clinical isolates from a tertiary referring hospital, depending on the richness of the environment and isolate serotypes, exhibited different, and sometimes controversial, virulence properties. The tested strains were susceptible to Ceftriaxone (90%) with decreasing reactivity to Trimethoprim–Sulfamethoxazole (72%), Chloramphenicol (64%), Ampicillin (48%), Gentamicin (44%), and Ciprofloxacin (2%). Disc susceptibility results partially correlated with minimum inhibitory concentration (MIC); however, special attention must be given to antimicrobial treatment, as a rise in multi-resistant isolates to Trimethoprim–Sulfamethoxazole (2/38 µg/mL), Minocycline (8 µg/mL) and Ampicillin (16 µg/mL) has been noticed, with two isolates resistant to Ceftazidime (16 µg/mL). By comparison to previous molecular epidemiology studies, the variation in the gene profiles of endemic pathogens supports the need for continuous and up-to-date microbiological and molecular reports. |
| first_indexed | 2024-03-09T04:37:25Z |
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| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T04:37:25Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-d47e9b575a4e4108aa6eb42c718211412023-12-03T13:27:11ZengMDPI AGPathogens2076-08172021-01-011017410.3390/pathogens10010074Correlation between Pathogenic Determinants Associated with Clinically Isolated Non-Typhoidal <i>Salmonella</i>Boimpoundi Eunice Flavie Ouali0Tsyr-Huei Chiou1Jenn-Wei Chen2I-Chu Lin3Ching-Chuan Liu4Yu-Chung Chiang5Tzong-Shiann Ho6Hao-Ven Wang7Department of Life Sciences, National Cheng Kung University, Tainan 701, TaiwanDepartment of Life Sciences, National Cheng Kung University, Tainan 701, TaiwanDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 701, TaiwanDepartment of Life Sciences, National Cheng Kung University, Tainan 701, TaiwanCenter of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan 701, TaiwanDepartment of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, TaiwanCenter of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan 701, TaiwanDepartment of Life Sciences, National Cheng Kung University, Tainan 701, TaiwanNon-typhoidal and Typhoidal <i>Salmonella</i> are bacterial pathogens source of worldwide and major disease burden. Virulent determinants of specific serovars belonging to non-typhoidal <i>Salmonella</i> have been extensively studied in different models, yet the pathogenesis of this group of bacteria and the development of clinical symptoms globally remains underexplored. Herein, we implemented microbiological and molecular procedures to investigate isolate virulence traits and molecular diversity, likely in association with disease severity. Our results show that selected clinical isolates from a tertiary referring hospital, depending on the richness of the environment and isolate serotypes, exhibited different, and sometimes controversial, virulence properties. The tested strains were susceptible to Ceftriaxone (90%) with decreasing reactivity to Trimethoprim–Sulfamethoxazole (72%), Chloramphenicol (64%), Ampicillin (48%), Gentamicin (44%), and Ciprofloxacin (2%). Disc susceptibility results partially correlated with minimum inhibitory concentration (MIC); however, special attention must be given to antimicrobial treatment, as a rise in multi-resistant isolates to Trimethoprim–Sulfamethoxazole (2/38 µg/mL), Minocycline (8 µg/mL) and Ampicillin (16 µg/mL) has been noticed, with two isolates resistant to Ceftazidime (16 µg/mL). By comparison to previous molecular epidemiology studies, the variation in the gene profiles of endemic pathogens supports the need for continuous and up-to-date microbiological and molecular reports.https://www.mdpi.com/2076-0817/10/1/74non-typhoidal <i>Salmonella</i>virulence factorpathogenicityclinical isolates |
| spellingShingle | Boimpoundi Eunice Flavie Ouali Tsyr-Huei Chiou Jenn-Wei Chen I-Chu Lin Ching-Chuan Liu Yu-Chung Chiang Tzong-Shiann Ho Hao-Ven Wang Correlation between Pathogenic Determinants Associated with Clinically Isolated Non-Typhoidal <i>Salmonella</i> Pathogens non-typhoidal <i>Salmonella</i> virulence factor pathogenicity clinical isolates |
| title | Correlation between Pathogenic Determinants Associated with Clinically Isolated Non-Typhoidal <i>Salmonella</i> |
| title_full | Correlation between Pathogenic Determinants Associated with Clinically Isolated Non-Typhoidal <i>Salmonella</i> |
| title_fullStr | Correlation between Pathogenic Determinants Associated with Clinically Isolated Non-Typhoidal <i>Salmonella</i> |
| title_full_unstemmed | Correlation between Pathogenic Determinants Associated with Clinically Isolated Non-Typhoidal <i>Salmonella</i> |
| title_short | Correlation between Pathogenic Determinants Associated with Clinically Isolated Non-Typhoidal <i>Salmonella</i> |
| title_sort | correlation between pathogenic determinants associated with clinically isolated non typhoidal i salmonella i |
| topic | non-typhoidal <i>Salmonella</i> virulence factor pathogenicity clinical isolates |
| url | https://www.mdpi.com/2076-0817/10/1/74 |
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