Role of Long Noncoding RNAs ZlMSTRG.11348 and UeMSTRG.02678 in Temperature-Dependent Culm Swelling in <i>Zizania latifolia</i>
Temperature influences the physiological processes and ecology of both hosts and endophytes; however, it remains unclear how long noncoding RNAs (lncRNAs) modulate the consequences of temperature-dependent changes in host–pathogen interactions. To explore the role of lncRNAs in culm gall formation i...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-06-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/11/6020 |
| Summary: | Temperature influences the physiological processes and ecology of both hosts and endophytes; however, it remains unclear how long noncoding RNAs (lncRNAs) modulate the consequences of temperature-dependent changes in host–pathogen interactions. To explore the role of lncRNAs in culm gall formation induced by the smut fungus <i>Ustilago esculenta</i> in <i>Zizania latifolia</i>, we employed RNA sequencing to identify lncRNAs and their potential <i>cis</i>-targets in <i>Z. latifolia</i> and <i>U. esculenta</i> under different temperatures. In <i>Z. latifolia</i> and <i>U. esculenta</i>, we identified 3194 and 173 lncRNAs as well as 126 and four potential target genes for differentially expressed lncRNAs, respectively. Further function and expression analysis revealed that lncRNA ZlMSTRG.11348 regulates amino acid metabolism in <i>Z. latifolia</i> and lncRNA UeMSTRG.02678 regulates amino acid transport in <i>U. esculenta</i>. The plant defence response was also found to be regulated by lncRNAs and suppressed in <i>Z. latifolia</i> infected with <i>U. esculenta</i> grown at 25 °C, which may result from the expression of effector genes in <i>U. esculenta</i>. Moreover, in <i>Z. latifolia</i> infected with <i>U. esculenta</i>, the expression of genes related to phytohormones was altered under different temperatures. Our results demonstrate that lncRNAs are important components of the regulatory networks in plant-microbe-environment interactions, and may play a part in regulating culm swelling in <i>Z. latifolia</i> plants. |
|---|---|
| ISSN: | 1661-6596 1422-0067 |