Pumpkin Seed Oil-Loaded Niosomes for Topical Application: 5α-Reductase Inhibitory, Anti-Inflammatory, and In Vivo Anti-Hair Loss Effects
Pumpkin seed oil (PSO)-loaded niosomes were prepared from Tween 20 and cholesterol by ethanol injection. Confocal microscopy showed better skin permeation and hair follicle accumulation of the niosomes compared to the PSO solution. The PSO-loaded niosomes inhibited 5α-reductase activity in DU-145 ce...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/8/930 |
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| author | Veerawat Teeranachaideekul Warisara Parichatikanond Varaporn Buraphacheep Junyaprasert Boontida Morakul |
| author_facet | Veerawat Teeranachaideekul Warisara Parichatikanond Varaporn Buraphacheep Junyaprasert Boontida Morakul |
| author_sort | Veerawat Teeranachaideekul |
| collection | DOAJ |
| description | Pumpkin seed oil (PSO)-loaded niosomes were prepared from Tween 20 and cholesterol by ethanol injection. Confocal microscopy showed better skin permeation and hair follicle accumulation of the niosomes compared to the PSO solution. The PSO-loaded niosomes inhibited 5α-reductase activity in DU-145 cells and hindered IL-6 activity in RAW 264.7 cells. These effects indicated the great potential of PSO-loaded niosomes to reduce hair loss. The hair scalp serum with PSO-loaded niosomes did not show irritation to reconstructed human skin. This formulation presented a significant decrease in the percentage of fallen hairs by 44.42% in the in vivo 60-second hair count experiment and a significant increase in the anagen to telogen (A/T) ratio (1.4-fold) in the TrichoScan<sup>®</sup> evaluation after 8 weeks of treatment compared to the initial conditions, indicating the promising efficacy of PSO-loaded niosomes as a natural alternative for anti-hair loss therapy. |
| first_indexed | 2024-03-09T12:48:26Z |
| format | Article |
| id | doaj.art-d483a48f1164445ca027a0c06a0d2bd9 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T12:48:26Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-d483a48f1164445ca027a0c06a0d2bd92023-11-30T22:09:56ZengMDPI AGPharmaceuticals1424-82472022-07-0115893010.3390/ph15080930Pumpkin Seed Oil-Loaded Niosomes for Topical Application: 5α-Reductase Inhibitory, Anti-Inflammatory, and In Vivo Anti-Hair Loss EffectsVeerawat Teeranachaideekul0Warisara Parichatikanond1Varaporn Buraphacheep Junyaprasert2Boontida Morakul3Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandDepartment of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandDepartment of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandDepartment of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandPumpkin seed oil (PSO)-loaded niosomes were prepared from Tween 20 and cholesterol by ethanol injection. Confocal microscopy showed better skin permeation and hair follicle accumulation of the niosomes compared to the PSO solution. The PSO-loaded niosomes inhibited 5α-reductase activity in DU-145 cells and hindered IL-6 activity in RAW 264.7 cells. These effects indicated the great potential of PSO-loaded niosomes to reduce hair loss. The hair scalp serum with PSO-loaded niosomes did not show irritation to reconstructed human skin. This formulation presented a significant decrease in the percentage of fallen hairs by 44.42% in the in vivo 60-second hair count experiment and a significant increase in the anagen to telogen (A/T) ratio (1.4-fold) in the TrichoScan<sup>®</sup> evaluation after 8 weeks of treatment compared to the initial conditions, indicating the promising efficacy of PSO-loaded niosomes as a natural alternative for anti-hair loss therapy.https://www.mdpi.com/1424-8247/15/8/930pumpkin seed oilniosomesanti-hair loss5α-reductase |
| spellingShingle | Veerawat Teeranachaideekul Warisara Parichatikanond Varaporn Buraphacheep Junyaprasert Boontida Morakul Pumpkin Seed Oil-Loaded Niosomes for Topical Application: 5α-Reductase Inhibitory, Anti-Inflammatory, and In Vivo Anti-Hair Loss Effects Pharmaceuticals pumpkin seed oil niosomes anti-hair loss 5α-reductase |
| title | Pumpkin Seed Oil-Loaded Niosomes for Topical Application: 5α-Reductase Inhibitory, Anti-Inflammatory, and In Vivo Anti-Hair Loss Effects |
| title_full | Pumpkin Seed Oil-Loaded Niosomes for Topical Application: 5α-Reductase Inhibitory, Anti-Inflammatory, and In Vivo Anti-Hair Loss Effects |
| title_fullStr | Pumpkin Seed Oil-Loaded Niosomes for Topical Application: 5α-Reductase Inhibitory, Anti-Inflammatory, and In Vivo Anti-Hair Loss Effects |
| title_full_unstemmed | Pumpkin Seed Oil-Loaded Niosomes for Topical Application: 5α-Reductase Inhibitory, Anti-Inflammatory, and In Vivo Anti-Hair Loss Effects |
| title_short | Pumpkin Seed Oil-Loaded Niosomes for Topical Application: 5α-Reductase Inhibitory, Anti-Inflammatory, and In Vivo Anti-Hair Loss Effects |
| title_sort | pumpkin seed oil loaded niosomes for topical application 5α reductase inhibitory anti inflammatory and in vivo anti hair loss effects |
| topic | pumpkin seed oil niosomes anti-hair loss 5α-reductase |
| url | https://www.mdpi.com/1424-8247/15/8/930 |
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