Successful cryopreservation and regeneration of a partridge colored Hungarian native chicken breed using primordial germ cells
ABSTRACT: Primordial germ cells (PGCs) are the precursors of germline cells that generate sperm and ova in adults. Thus, they are promising tools for gene editing and genetic preservation, especially in avian species. In this study, we established stable male and female PGC lines from 6Hungarian ind...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-08-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579121002418 |
| _version_ | 1819139951366766592 |
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| author | Bence Lázár Mariann Molnár Nikoletta Sztán Barbara Végi Árpád Drobnyák Roland Tóth Nikolett Tokodyné Szabadi Michael J. McGrew Elen Gócza Eszter Patakiné Várkonyi |
| author_facet | Bence Lázár Mariann Molnár Nikoletta Sztán Barbara Végi Árpád Drobnyák Roland Tóth Nikolett Tokodyné Szabadi Michael J. McGrew Elen Gócza Eszter Patakiné Várkonyi |
| author_sort | Bence Lázár |
| collection | DOAJ |
| description | ABSTRACT: Primordial germ cells (PGCs) are the precursors of germline cells that generate sperm and ova in adults. Thus, they are promising tools for gene editing and genetic preservation, especially in avian species. In this study, we established stable male and female PGC lines from 6Hungarian indigenous chicken breeds with derivation rates ranging from 37.5 to 50 percent. We characterized the PGCs for expression of the germ cell-specific markers during prolonged culture in vitro. An in vivo colonization test was performed on PGCs from four Hungarian chicken breeds and the colonization rates were between 76 and 100%. Cryopreserved PGCs of the donor breed (Partridge color Hungarian) were injected into Black Transylvanian Naked Neck host embryos to form chimeric progeny that, after backcrossing, would permit reconstitution of the donor breed. For 24 presumptive chimeras 13 were male and 11 were female. In the course of backcrossing, 340 chicks were hatched and 17 of them (5%) were pure Partridge colored. Based on the backcrossing 1 hen and 3 roosters of the 24 presumptive chimeras (16.6%) have proven to be germline chimeras. Therefore, it was proven that the original breed can be recovered from primordial germ cells which are stored in the gene bank. To our knowledge, our study is a first that applied feeder free culturing conditions for both male and female cell lines successfully and used multiple indigenous chicken breeds to create a gene bank representing a region (Carpathian Basin). |
| first_indexed | 2024-12-22T11:30:49Z |
| format | Article |
| id | doaj.art-d4852e0bd5294fc9b1f0280db2e2b5e6 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-22T11:30:49Z |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-d4852e0bd5294fc9b1f0280db2e2b5e62022-12-21T18:27:38ZengElsevierPoultry Science0032-57912021-08-011008101207Successful cryopreservation and regeneration of a partridge colored Hungarian native chicken breed using primordial germ cellsBence Lázár0Mariann Molnár1Nikoletta Sztán2Barbara Végi3Árpád Drobnyák4Roland Tóth5Nikolett Tokodyné Szabadi6Michael J. McGrew7Elen Gócza8Eszter Patakiné Várkonyi9National Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, 200 Isaszegi street, 2100 Gödöllő, Hungary; Hungarian University of Agriculture and Life Sciences, Institute of Genetics and Biotechnology, Animal Biotechnology Department, 4 Szent-Györgyi Albert street, 2100 Gödöllő, Hungary; Corresponding author:National Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, 200 Isaszegi street, 2100 Gödöllő, HungaryNational Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, 200 Isaszegi street, 2100 Gödöllő, HungaryNational Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, 200 Isaszegi street, 2100 Gödöllő, HungaryNational Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, 200 Isaszegi street, 2100 Gödöllő, HungaryHungarian University of Agriculture and Life Sciences, Institute of Genetics and Biotechnology, Animal Biotechnology Department, 4 Szent-Györgyi Albert street, 2100 Gödöllő, HungaryHungarian University of Agriculture and Life Sciences, Institute of Genetics and Biotechnology, Animal Biotechnology Department, 4 Szent-Györgyi Albert street, 2100 Gödöllő, HungaryThe Roslin Institute and Royal Dick School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, EH25 9RG, Midlothian, UKHungarian University of Agriculture and Life Sciences, Institute of Genetics and Biotechnology, Animal Biotechnology Department, 4 Szent-Györgyi Albert street, 2100 Gödöllő, HungaryNational Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, 200 Isaszegi street, 2100 Gödöllő, HungaryABSTRACT: Primordial germ cells (PGCs) are the precursors of germline cells that generate sperm and ova in adults. Thus, they are promising tools for gene editing and genetic preservation, especially in avian species. In this study, we established stable male and female PGC lines from 6Hungarian indigenous chicken breeds with derivation rates ranging from 37.5 to 50 percent. We characterized the PGCs for expression of the germ cell-specific markers during prolonged culture in vitro. An in vivo colonization test was performed on PGCs from four Hungarian chicken breeds and the colonization rates were between 76 and 100%. Cryopreserved PGCs of the donor breed (Partridge color Hungarian) were injected into Black Transylvanian Naked Neck host embryos to form chimeric progeny that, after backcrossing, would permit reconstitution of the donor breed. For 24 presumptive chimeras 13 were male and 11 were female. In the course of backcrossing, 340 chicks were hatched and 17 of them (5%) were pure Partridge colored. Based on the backcrossing 1 hen and 3 roosters of the 24 presumptive chimeras (16.6%) have proven to be germline chimeras. Therefore, it was proven that the original breed can be recovered from primordial germ cells which are stored in the gene bank. To our knowledge, our study is a first that applied feeder free culturing conditions for both male and female cell lines successfully and used multiple indigenous chicken breeds to create a gene bank representing a region (Carpathian Basin).http://www.sciencedirect.com/science/article/pii/S0032579121002418PGC lineindigenous chicken breedgermline transmissioncryopreservationgene bank |
| spellingShingle | Bence Lázár Mariann Molnár Nikoletta Sztán Barbara Végi Árpád Drobnyák Roland Tóth Nikolett Tokodyné Szabadi Michael J. McGrew Elen Gócza Eszter Patakiné Várkonyi Successful cryopreservation and regeneration of a partridge colored Hungarian native chicken breed using primordial germ cells Poultry Science PGC line indigenous chicken breed germline transmission cryopreservation gene bank |
| title | Successful cryopreservation and regeneration of a partridge colored Hungarian native chicken breed using primordial germ cells |
| title_full | Successful cryopreservation and regeneration of a partridge colored Hungarian native chicken breed using primordial germ cells |
| title_fullStr | Successful cryopreservation and regeneration of a partridge colored Hungarian native chicken breed using primordial germ cells |
| title_full_unstemmed | Successful cryopreservation and regeneration of a partridge colored Hungarian native chicken breed using primordial germ cells |
| title_short | Successful cryopreservation and regeneration of a partridge colored Hungarian native chicken breed using primordial germ cells |
| title_sort | successful cryopreservation and regeneration of a partridge colored hungarian native chicken breed using primordial germ cells |
| topic | PGC line indigenous chicken breed germline transmission cryopreservation gene bank |
| url | http://www.sciencedirect.com/science/article/pii/S0032579121002418 |
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