Effects of Aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghum
IntroductionCyanogenic glycosides (CNglcs) are bioactive plant products involving in plant defense against herbivores by virtue of their abilities to release toxic hydrogen cyanide (HCN). Aspergillus niger has been shown to be effective in producing β-glucosidase, which could degrade CNglcs. However...
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128057/full |
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| author | Jianrong Zhai Jianrong Zhai Jianrong Zhai Bo Wang Bo Wang Bo Wang Yingpeng Sun Yingpeng Sun Yingpeng Sun Jianfeng Yang Jianfeng Yang Jianfeng Yang Junfeng Zhou Tianyu Wang Tianyu Wang Tianyu Wang Wenlan Zhang Wenlan Zhang Wenlan Zhang Cai Qi Yanjun Guo Yanjun Guo Yanjun Guo |
| author_facet | Jianrong Zhai Jianrong Zhai Jianrong Zhai Bo Wang Bo Wang Bo Wang Yingpeng Sun Yingpeng Sun Yingpeng Sun Jianfeng Yang Jianfeng Yang Jianfeng Yang Junfeng Zhou Tianyu Wang Tianyu Wang Tianyu Wang Wenlan Zhang Wenlan Zhang Wenlan Zhang Cai Qi Yanjun Guo Yanjun Guo Yanjun Guo |
| author_sort | Jianrong Zhai |
| collection | DOAJ |
| description | IntroductionCyanogenic glycosides (CNglcs) are bioactive plant products involving in plant defense against herbivores by virtue of their abilities to release toxic hydrogen cyanide (HCN). Aspergillus niger has been shown to be effective in producing β-glucosidase, which could degrade CNglcs. However, whether A. niger could remove CNglcs under ensiling conditions is still unknown.MethodsIn this study, we first investigated the HCN contents in ratooning sorghums for two years, then the sorghums were ensiled with or without the addition of A. niger.ResultsTwo years’ investigation indicated that the contents of HCN in fresh ratooning sorghum were larger than 801 mg/kg FW (fresh weight), which could not be reduced by silage fermentation under safety threshold (200 mg/kg FW). A. niger could produce β-glucosidase over a range of pH and temperature, which degraded the CNglcs and removed the hydrogen cyanide (HCN) at early days of ratooning sorghum fermentation. The addition of A. niger (2.56 × 107 CFU/ml) altered the microbial community, increased bacterial diversity, improved the nutritive qualities, and reduced the HCN contents in ensiled ratooning sorghum lower than 100 mg/kg FW after 60 days of fermentation. Overall, the addition of 150 ml A. niger + 50 ml sterile water per 3 kg silage could efficiently remove CNglcs from ratooning sorghum silage.ConclusionIn conclusion, A. niger could produce β-glucosidase which degraded the CNglcs during the early days of fermentation, benefiting the ensiling process and improving the utilization of ratooning sorghum. |
| first_indexed | 2024-04-10T09:24:48Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T09:24:48Z |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-d487323e06a94201a2b404306c767d962023-02-20T06:29:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011410.3389/fmicb.2023.11280571128057Effects of Aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghumJianrong Zhai0Jianrong Zhai1Jianrong Zhai2Bo Wang3Bo Wang4Bo Wang5Yingpeng Sun6Yingpeng Sun7Yingpeng Sun8Jianfeng Yang9Jianfeng Yang10Jianfeng Yang11Junfeng Zhou12Tianyu Wang13Tianyu Wang14Tianyu Wang15Wenlan Zhang16Wenlan Zhang17Wenlan Zhang18Cai Qi19Yanjun Guo20Yanjun Guo21Yanjun Guo22Qingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach, Qingdao Agricultural University, Qingdao, ChinaKey Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao Agricultural University, Qingdao, ChinaCollege of Animal Science and Technology, Southwest University, Chongqing, ChinaQingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach, Qingdao Agricultural University, Qingdao, ChinaKey Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao Agricultural University, Qingdao, ChinaCollege of Grassland Science, Qingdao Agricultural University, Qingdao, ChinaQingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach, Qingdao Agricultural University, Qingdao, ChinaKey Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao Agricultural University, Qingdao, ChinaCollege of Grassland Science, Qingdao Agricultural University, Qingdao, ChinaQingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach, Qingdao Agricultural University, Qingdao, ChinaKey Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao Agricultural University, Qingdao, ChinaCollege of Animal Science and Technology, Southwest University, Chongqing, ChinaChongqing Jiangxiaobai Farm Co., Ltd., Chongqing, ChinaQingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach, Qingdao Agricultural University, Qingdao, ChinaKey Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao Agricultural University, Qingdao, ChinaCollege of Grassland Science, Qingdao Agricultural University, Qingdao, ChinaQingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach, Qingdao Agricultural University, Qingdao, ChinaKey Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao Agricultural University, Qingdao, ChinaCollege of Grassland Science, Qingdao Agricultural University, Qingdao, ChinaCollege of Animal Science and Technology, Southwest University, Chongqing, ChinaQingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach, Qingdao Agricultural University, Qingdao, ChinaKey Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao Agricultural University, Qingdao, ChinaCollege of Grassland Science, Qingdao Agricultural University, Qingdao, ChinaIntroductionCyanogenic glycosides (CNglcs) are bioactive plant products involving in plant defense against herbivores by virtue of their abilities to release toxic hydrogen cyanide (HCN). Aspergillus niger has been shown to be effective in producing β-glucosidase, which could degrade CNglcs. However, whether A. niger could remove CNglcs under ensiling conditions is still unknown.MethodsIn this study, we first investigated the HCN contents in ratooning sorghums for two years, then the sorghums were ensiled with or without the addition of A. niger.ResultsTwo years’ investigation indicated that the contents of HCN in fresh ratooning sorghum were larger than 801 mg/kg FW (fresh weight), which could not be reduced by silage fermentation under safety threshold (200 mg/kg FW). A. niger could produce β-glucosidase over a range of pH and temperature, which degraded the CNglcs and removed the hydrogen cyanide (HCN) at early days of ratooning sorghum fermentation. The addition of A. niger (2.56 × 107 CFU/ml) altered the microbial community, increased bacterial diversity, improved the nutritive qualities, and reduced the HCN contents in ensiled ratooning sorghum lower than 100 mg/kg FW after 60 days of fermentation. Overall, the addition of 150 ml A. niger + 50 ml sterile water per 3 kg silage could efficiently remove CNglcs from ratooning sorghum silage.ConclusionIn conclusion, A. niger could produce β-glucosidase which degraded the CNglcs during the early days of fermentation, benefiting the ensiling process and improving the utilization of ratooning sorghum.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128057/fullAspergillus nigercyanogenic glycosideshydrocyanic acidratooning sorghumsilage |
| spellingShingle | Jianrong Zhai Jianrong Zhai Jianrong Zhai Bo Wang Bo Wang Bo Wang Yingpeng Sun Yingpeng Sun Yingpeng Sun Jianfeng Yang Jianfeng Yang Jianfeng Yang Junfeng Zhou Tianyu Wang Tianyu Wang Tianyu Wang Wenlan Zhang Wenlan Zhang Wenlan Zhang Cai Qi Yanjun Guo Yanjun Guo Yanjun Guo Effects of Aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghum Frontiers in Microbiology Aspergillus niger cyanogenic glycosides hydrocyanic acid ratooning sorghum silage |
| title | Effects of Aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghum |
| title_full | Effects of Aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghum |
| title_fullStr | Effects of Aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghum |
| title_full_unstemmed | Effects of Aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghum |
| title_short | Effects of Aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghum |
| title_sort | effects of aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghum |
| topic | Aspergillus niger cyanogenic glycosides hydrocyanic acid ratooning sorghum silage |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128057/full |
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