A Paper-Based Colorimetric Aptasensor for the Detection of Gentamicin
Antibiotics are classes of antimicrobial substances that are administered widely in the field of veterinary science to promote animal health and feed efficiency. Cattle-administered antibiotics hold a risk of passing active residues to milk, during the milking process. This becomes a public health c...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/2/29 |
| _version_ | 1797408943946858496 |
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| author | Saipriya Ramalingam Christopher M. Collier Ashutosh Singh |
| author_facet | Saipriya Ramalingam Christopher M. Collier Ashutosh Singh |
| author_sort | Saipriya Ramalingam |
| collection | DOAJ |
| description | Antibiotics are classes of antimicrobial substances that are administered widely in the field of veterinary science to promote animal health and feed efficiency. Cattle-administered antibiotics hold a risk of passing active residues to milk, during the milking process. This becomes a public health concern as these residues can cause severe allergic reactions to sensitive groups and considerable economic losses to the farmer. Hence, to ensure that the produced milk is safe to consume and adheres to permissible limits, an on-farm quick and reliable test is essential. This study illustrates the design and development of a microfluidic paper biosensor as a proof-of-concept detection system for gentamicin in milk. Localized surface plasmon resonance (LSPR) properties of gold nanoparticles have been explored to provide the user a visual feedback on the test, which was also corroborated by RGB analysis performed using Image J. The assay involves the use of a short stretch of single stranded DNA, called aptamer, which is very specific to the gentamicin present in the milk sample. The camera-based LOD for the fabricated paper device for milk samples spiked with gentamicin was calculated to be 300 nM, with a reaction time of 2 min. |
| first_indexed | 2024-03-09T04:07:00Z |
| format | Article |
| id | doaj.art-d487a483e10141e799b75d48ce2748e6 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T04:07:00Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-d487a483e10141e799b75d48ce2748e62023-12-03T14:05:55ZengMDPI AGBiosensors2079-63742021-01-011122910.3390/bios11020029A Paper-Based Colorimetric Aptasensor for the Detection of GentamicinSaipriya Ramalingam0Christopher M. Collier1Ashutosh Singh2School of Engineering, University of Guelph, Guelph, ON N1G 2W1, CanadaSchool of Engineering, University of Guelph, Guelph, ON N1G 2W1, CanadaSchool of Engineering, University of Guelph, Guelph, ON N1G 2W1, CanadaAntibiotics are classes of antimicrobial substances that are administered widely in the field of veterinary science to promote animal health and feed efficiency. Cattle-administered antibiotics hold a risk of passing active residues to milk, during the milking process. This becomes a public health concern as these residues can cause severe allergic reactions to sensitive groups and considerable economic losses to the farmer. Hence, to ensure that the produced milk is safe to consume and adheres to permissible limits, an on-farm quick and reliable test is essential. This study illustrates the design and development of a microfluidic paper biosensor as a proof-of-concept detection system for gentamicin in milk. Localized surface plasmon resonance (LSPR) properties of gold nanoparticles have been explored to provide the user a visual feedback on the test, which was also corroborated by RGB analysis performed using Image J. The assay involves the use of a short stretch of single stranded DNA, called aptamer, which is very specific to the gentamicin present in the milk sample. The camera-based LOD for the fabricated paper device for milk samples spiked with gentamicin was calculated to be 300 nM, with a reaction time of 2 min.https://www.mdpi.com/2079-6374/11/2/29gentamicinaptamergold nanoparticlescolorimetric biosensorpaper microfluidicsaptamer |
| spellingShingle | Saipriya Ramalingam Christopher M. Collier Ashutosh Singh A Paper-Based Colorimetric Aptasensor for the Detection of Gentamicin Biosensors gentamicin aptamer gold nanoparticles colorimetric biosensor paper microfluidics aptamer |
| title | A Paper-Based Colorimetric Aptasensor for the Detection of Gentamicin |
| title_full | A Paper-Based Colorimetric Aptasensor for the Detection of Gentamicin |
| title_fullStr | A Paper-Based Colorimetric Aptasensor for the Detection of Gentamicin |
| title_full_unstemmed | A Paper-Based Colorimetric Aptasensor for the Detection of Gentamicin |
| title_short | A Paper-Based Colorimetric Aptasensor for the Detection of Gentamicin |
| title_sort | paper based colorimetric aptasensor for the detection of gentamicin |
| topic | gentamicin aptamer gold nanoparticles colorimetric biosensor paper microfluidics aptamer |
| url | https://www.mdpi.com/2079-6374/11/2/29 |
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