NiO Nanoparticles for Electrochemical Insulin Detection
Diabetes mellitus represents one of the most widespread diseases in civilization nowadays. Since the costs for treating and diagnosing of diabetes represent several billions of dollars per year, a cheap, fast, and simple sensor for diabetes diagnosis is needed. Electrochemical insulin sensors can be...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/21/15/5063 |
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| author | Jana Shepa Ivana Šišoláková Marek Vojtko Libuše Trnková Géza Nagy Iveta Maskaľová Andrej Oriňak Renáta Oriňaková |
| author_facet | Jana Shepa Ivana Šišoláková Marek Vojtko Libuše Trnková Géza Nagy Iveta Maskaľová Andrej Oriňak Renáta Oriňaková |
| author_sort | Jana Shepa |
| collection | DOAJ |
| description | Diabetes mellitus represents one of the most widespread diseases in civilization nowadays. Since the costs for treating and diagnosing of diabetes represent several billions of dollars per year, a cheap, fast, and simple sensor for diabetes diagnosis is needed. Electrochemical insulin sensors can be considered as a novel approach for diabetes diagnosis. In this study, carbon electrode with electrodeposited NiO nanoparticles was selected as a suitable electrode material for insulin determination. The morphology and surface composition were studied by scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, and X-ray photoelectron spectroscopy (XPS). For a better understanding of insulin determination on NiO-modified electrodes, the mechanism of electrochemical reaction and the kinetic parameters were studied. They were calculated from both voltammetric and amperometric measurements. The modified carbon electrode displayed a wide linear range from 600 nM to 10 µM, a low limit of detection of 19.6 nM, and a high sensitivity of 7.06 µA/µM. The electrodes were stable for 30 cycles and were able to detect insulin even in bovine blood serum. Additionally, the temperature stability of this electrode and its storage conditions were studied with appropriate outcomes. The above results show the high promise of this electrode for detecting insulin in clinical samples. |
| first_indexed | 2024-03-10T09:09:33Z |
| format | Article |
| id | doaj.art-d48f3273bd74475f9a9fa69ba15e344a |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T09:09:33Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-d48f3273bd74475f9a9fa69ba15e344a2023-11-22T06:09:57ZengMDPI AGSensors1424-82202021-07-012115506310.3390/s21155063NiO Nanoparticles for Electrochemical Insulin DetectionJana Shepa0Ivana Šišoláková1Marek Vojtko2Libuše Trnková3Géza Nagy4Iveta Maskaľová5Andrej Oriňak6Renáta Oriňaková7Department of Physical Chemistry, P.J. Šafárik University in Košice, Moyzesová 11, 040 01 Košice, SlovakiaDepartment of Physical Chemistry, P.J. Šafárik University in Košice, Moyzesová 11, 040 01 Košice, SlovakiaInstitute of Materials Research, Slovak Academy of Science, Watsonova 47, 040 01 Košice, SlovakiaDepartment of Chemistry, Masaryk University, Kamenice 5, 625 00 Brno, Czech RepublicDepartment of General and Physical Chemistry, University of Pécs, Ifjúság útja 6, 7624 Pécs, HungaryDepartment of Nutrition, Dietetics, and Animal Breeding, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, SlovakiaDepartment of Physical Chemistry, P.J. Šafárik University in Košice, Moyzesová 11, 040 01 Košice, SlovakiaDepartment of Physical Chemistry, P.J. Šafárik University in Košice, Moyzesová 11, 040 01 Košice, SlovakiaDiabetes mellitus represents one of the most widespread diseases in civilization nowadays. Since the costs for treating and diagnosing of diabetes represent several billions of dollars per year, a cheap, fast, and simple sensor for diabetes diagnosis is needed. Electrochemical insulin sensors can be considered as a novel approach for diabetes diagnosis. In this study, carbon electrode with electrodeposited NiO nanoparticles was selected as a suitable electrode material for insulin determination. The morphology and surface composition were studied by scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, and X-ray photoelectron spectroscopy (XPS). For a better understanding of insulin determination on NiO-modified electrodes, the mechanism of electrochemical reaction and the kinetic parameters were studied. They were calculated from both voltammetric and amperometric measurements. The modified carbon electrode displayed a wide linear range from 600 nM to 10 µM, a low limit of detection of 19.6 nM, and a high sensitivity of 7.06 µA/µM. The electrodes were stable for 30 cycles and were able to detect insulin even in bovine blood serum. Additionally, the temperature stability of this electrode and its storage conditions were studied with appropriate outcomes. The above results show the high promise of this electrode for detecting insulin in clinical samples.https://www.mdpi.com/1424-8220/21/15/5063NiO nanoparticlesinsulinelectrochemical sensor |
| spellingShingle | Jana Shepa Ivana Šišoláková Marek Vojtko Libuše Trnková Géza Nagy Iveta Maskaľová Andrej Oriňak Renáta Oriňaková NiO Nanoparticles for Electrochemical Insulin Detection Sensors NiO nanoparticles insulin electrochemical sensor |
| title | NiO Nanoparticles for Electrochemical Insulin Detection |
| title_full | NiO Nanoparticles for Electrochemical Insulin Detection |
| title_fullStr | NiO Nanoparticles for Electrochemical Insulin Detection |
| title_full_unstemmed | NiO Nanoparticles for Electrochemical Insulin Detection |
| title_short | NiO Nanoparticles for Electrochemical Insulin Detection |
| title_sort | nio nanoparticles for electrochemical insulin detection |
| topic | NiO nanoparticles insulin electrochemical sensor |
| url | https://www.mdpi.com/1424-8220/21/15/5063 |
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