Anodic and Cathodic Extracellular Electron Transfer by the Filamentous Bacterium Ardenticatena maritima 110S
Ardenticatena maritima strain 110S is a filamentous bacterium isolated from an iron-rich coastal hydrothermal field, and it is a unique isolate capable of dissimilatory iron or nitrate reduction among the members of the bacterial phylum Chloroflexi. Here, we report the ability of A. maritima strain...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00068/full |
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| author | Satoshi Kawaichi Tetsuya Yamada Tetsuya Yamada Akio Umezawa Shawn E. McGlynn Shawn E. McGlynn Takehiro Suzuki Naoshi Dohmae Takashi Yoshida Yoshihiko Sako Nobuhiro Matsushita Kazuhito Hashimoto Ryuhei Nakamura Ryuhei Nakamura |
| author_facet | Satoshi Kawaichi Tetsuya Yamada Tetsuya Yamada Akio Umezawa Shawn E. McGlynn Shawn E. McGlynn Takehiro Suzuki Naoshi Dohmae Takashi Yoshida Yoshihiko Sako Nobuhiro Matsushita Kazuhito Hashimoto Ryuhei Nakamura Ryuhei Nakamura |
| author_sort | Satoshi Kawaichi |
| collection | DOAJ |
| description | Ardenticatena maritima strain 110S is a filamentous bacterium isolated from an iron-rich coastal hydrothermal field, and it is a unique isolate capable of dissimilatory iron or nitrate reduction among the members of the bacterial phylum Chloroflexi. Here, we report the ability of A. maritima strain 110S to utilize electrodes as a sole electron acceptor and donor when coupled with the oxidation of organic compounds and nitrate reduction, respectively. In addition, multicellular filaments with hundreds of cells arranged end-to-end increased the extracellular electron transfer (EET) ability to electrodes by organizing filaments into bundled structures, with the aid of microbially reduced iron oxide minerals on the cell surface of strain 110S. Based on these findings, together with the attempt to detect surface-localized cytochromes in the genome sequence and the demonstration of redox-dependent staining and immunostaining of the cell surface, we propose a model of bidirectional electron transport by A. maritima strain 110S, in which surface-localized multiheme cytochromes and surface-associated iron minerals serve as a conduit of bidirectional EET in multicellular filaments. |
| first_indexed | 2024-12-23T21:32:07Z |
| format | Article |
| id | doaj.art-d4905b63f9db4250ad04aec012b80c07 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T21:32:07Z |
| publishDate | 2018-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d4905b63f9db4250ad04aec012b80c072022-12-21T17:30:26ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-02-01910.3389/fmicb.2018.00068315537Anodic and Cathodic Extracellular Electron Transfer by the Filamentous Bacterium Ardenticatena maritima 110SSatoshi Kawaichi0Tetsuya Yamada1Tetsuya Yamada2Akio Umezawa3Shawn E. McGlynn4Shawn E. McGlynn5Takehiro Suzuki6Naoshi Dohmae7Takashi Yoshida8Yoshihiko Sako9Nobuhiro Matsushita10Kazuhito Hashimoto11Ryuhei Nakamura12Ryuhei Nakamura13Biofunctional Catalyst Research Team, Center for Sustainable Resource Science, RIKEN, Saitama, JapanBiofunctional Catalyst Research Team, Center for Sustainable Resource Science, RIKEN, Saitama, JapanDepartment of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo, JapanBiofunctional Catalyst Research Team, Center for Sustainable Resource Science, RIKEN, Saitama, JapanBiofunctional Catalyst Research Team, Center for Sustainable Resource Science, RIKEN, Saitama, JapanEarth-Life Science Institute, Tokyo Institute of Technology, Tokyo, JapanBiomolecular Characterization Unit, Center for Sustainable Resource Science, RIKEN, Wako, JapanBiomolecular Characterization Unit, Center for Sustainable Resource Science, RIKEN, Wako, JapanLaboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, JapanLaboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, JapanDepartment of Materials Science and Engineering, Tokyo Institute of Technology, Tokyo, JapanNational Institute for Materials Science, Tsukuba, JapanBiofunctional Catalyst Research Team, Center for Sustainable Resource Science, RIKEN, Saitama, JapanEarth-Life Science Institute, Tokyo Institute of Technology, Tokyo, JapanArdenticatena maritima strain 110S is a filamentous bacterium isolated from an iron-rich coastal hydrothermal field, and it is a unique isolate capable of dissimilatory iron or nitrate reduction among the members of the bacterial phylum Chloroflexi. Here, we report the ability of A. maritima strain 110S to utilize electrodes as a sole electron acceptor and donor when coupled with the oxidation of organic compounds and nitrate reduction, respectively. In addition, multicellular filaments with hundreds of cells arranged end-to-end increased the extracellular electron transfer (EET) ability to electrodes by organizing filaments into bundled structures, with the aid of microbially reduced iron oxide minerals on the cell surface of strain 110S. Based on these findings, together with the attempt to detect surface-localized cytochromes in the genome sequence and the demonstration of redox-dependent staining and immunostaining of the cell surface, we propose a model of bidirectional electron transport by A. maritima strain 110S, in which surface-localized multiheme cytochromes and surface-associated iron minerals serve as a conduit of bidirectional EET in multicellular filaments.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00068/fullextracellular electron transferfilamentous bacteriairon reducing bacterianitratecytochromes |
| spellingShingle | Satoshi Kawaichi Tetsuya Yamada Tetsuya Yamada Akio Umezawa Shawn E. McGlynn Shawn E. McGlynn Takehiro Suzuki Naoshi Dohmae Takashi Yoshida Yoshihiko Sako Nobuhiro Matsushita Kazuhito Hashimoto Ryuhei Nakamura Ryuhei Nakamura Anodic and Cathodic Extracellular Electron Transfer by the Filamentous Bacterium Ardenticatena maritima 110S Frontiers in Microbiology extracellular electron transfer filamentous bacteria iron reducing bacteria nitrate cytochromes |
| title | Anodic and Cathodic Extracellular Electron Transfer by the Filamentous Bacterium Ardenticatena maritima 110S |
| title_full | Anodic and Cathodic Extracellular Electron Transfer by the Filamentous Bacterium Ardenticatena maritima 110S |
| title_fullStr | Anodic and Cathodic Extracellular Electron Transfer by the Filamentous Bacterium Ardenticatena maritima 110S |
| title_full_unstemmed | Anodic and Cathodic Extracellular Electron Transfer by the Filamentous Bacterium Ardenticatena maritima 110S |
| title_short | Anodic and Cathodic Extracellular Electron Transfer by the Filamentous Bacterium Ardenticatena maritima 110S |
| title_sort | anodic and cathodic extracellular electron transfer by the filamentous bacterium ardenticatena maritima 110s |
| topic | extracellular electron transfer filamentous bacteria iron reducing bacteria nitrate cytochromes |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00068/full |
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