Ultrasonic-assisted synthesis of lignin-capped Cu2O nanocomposite with antibiofilm properties
Under ultrasonication, cuprous oxide (Cu2O) microparticles (<5 µm) were fragmented into nanoparticles (NPs, ranging from 10 to 30 nm in diameter), and interacted strongly with alkali lignin (Mw = 10 kDa) to form a nanocomposite. The ultrasonic wave generates strong binding interaction between lig...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417722003376 |
| _version_ | 1797956267411505152 |
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| author | Moorthy Maruthapandi Akanksha Gupta Arumugam Saravanan Gila Jacobi Ehud Banin John H.T. Luong Aharon Gedanken |
| author_facet | Moorthy Maruthapandi Akanksha Gupta Arumugam Saravanan Gila Jacobi Ehud Banin John H.T. Luong Aharon Gedanken |
| author_sort | Moorthy Maruthapandi |
| collection | DOAJ |
| description | Under ultrasonication, cuprous oxide (Cu2O) microparticles (<5 µm) were fragmented into nanoparticles (NPs, ranging from 10 to 30 nm in diameter), and interacted strongly with alkali lignin (Mw = 10 kDa) to form a nanocomposite. The ultrasonic wave generates strong binding interaction between lignin and Cu2O. The L-Cu nanocomposite exhibited synergistic effects with enhanced antibiofilm activities against E. coli, multidrug-resistant (MDR) E. coli, S. aureus (SA), methicillin-resistant SA, and P. aeruginosa (PA). The lignin-Cu2O (L-Cu) nanocomposite also imparted notable eradication of such bacterial biofilms. Experimental evidence unraveled the destruction of bacterial cell walls by L-Cu, which interacted strongly with the bacterial membrane. After exposure to L-Cu, the bacterial cells lost the integrated structural morphology. The estimated MIC for biofilm inhibition for the five tested pathogens was 1 mg/mL L-Cu (92 % lignin and 8 % Cu2ONPs, w/w %). The MIC for bacterial eradication was noticeably lower; 0.3 mg/mL (87 % lignin + 13 % Cu2ONPs, w/w %) for PA and SA, whereas this value was appreciably higher for MDR E. coli (0.56 mg/mL, 86 % lignin and 14 % Cu2O NPs). Such results highlighted the potential of L-Cu as an alternative to neutralize MDR pathogens. |
| first_indexed | 2024-04-10T23:47:22Z |
| format | Article |
| id | doaj.art-d4907f41f2da4b5d8d386ac97973e480 |
| institution | Directory Open Access Journal |
| issn | 1350-4177 |
| language | English |
| last_indexed | 2024-04-10T23:47:22Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj.art-d4907f41f2da4b5d8d386ac97973e4802023-01-11T04:27:46ZengElsevierUltrasonics Sonochemistry1350-41772023-01-0192106241Ultrasonic-assisted synthesis of lignin-capped Cu2O nanocomposite with antibiofilm propertiesMoorthy Maruthapandi0Akanksha Gupta1Arumugam Saravanan2Gila Jacobi3Ehud Banin4John H.T. Luong5Aharon Gedanken6Bar-Ilan Institute for Nanotechnology and Advanced Materials (BINA), Bar-Ilan University, Ramat-Gan 5290002, Israel; Department of Chemistry, Bar-Ilan University, Ramat-Gan 5290002, IsraelBar-Ilan Institute for Nanotechnology and Advanced Materials (BINA), Bar-Ilan University, Ramat-Gan 5290002, Israel; Department of Chemistry, Bar-Ilan University, Ramat-Gan 5290002, IsraelBar-Ilan Institute for Nanotechnology and Advanced Materials (BINA), Bar-Ilan University, Ramat-Gan 5290002, Israel; Department of Chemistry, Bar-Ilan University, Ramat-Gan 5290002, IsraelThe Mina and Everard Goodman Faculty of Life Sciences, Institute for Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 52900, IsraelThe Mina and Everard Goodman Faculty of Life Sciences, Institute for Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 52900, IsraelSchool of Chemistry, University College Cork, Cork T12 YN60, IrelandBar-Ilan Institute for Nanotechnology and Advanced Materials (BINA), Bar-Ilan University, Ramat-Gan 5290002, Israel; Department of Chemistry, Bar-Ilan University, Ramat-Gan 5290002, Israel; Corresponding author at: Bar-Ilan Institute for Nanotechnology and Advanced Materials (BINA), Bar-Ilan University, Ramat-Gan 5290002, Israel.Under ultrasonication, cuprous oxide (Cu2O) microparticles (<5 µm) were fragmented into nanoparticles (NPs, ranging from 10 to 30 nm in diameter), and interacted strongly with alkali lignin (Mw = 10 kDa) to form a nanocomposite. The ultrasonic wave generates strong binding interaction between lignin and Cu2O. The L-Cu nanocomposite exhibited synergistic effects with enhanced antibiofilm activities against E. coli, multidrug-resistant (MDR) E. coli, S. aureus (SA), methicillin-resistant SA, and P. aeruginosa (PA). The lignin-Cu2O (L-Cu) nanocomposite also imparted notable eradication of such bacterial biofilms. Experimental evidence unraveled the destruction of bacterial cell walls by L-Cu, which interacted strongly with the bacterial membrane. After exposure to L-Cu, the bacterial cells lost the integrated structural morphology. The estimated MIC for biofilm inhibition for the five tested pathogens was 1 mg/mL L-Cu (92 % lignin and 8 % Cu2ONPs, w/w %). The MIC for bacterial eradication was noticeably lower; 0.3 mg/mL (87 % lignin + 13 % Cu2ONPs, w/w %) for PA and SA, whereas this value was appreciably higher for MDR E. coli (0.56 mg/mL, 86 % lignin and 14 % Cu2O NPs). Such results highlighted the potential of L-Cu as an alternative to neutralize MDR pathogens.http://www.sciencedirect.com/science/article/pii/S1350417722003376Ultrasonic methodLignin Cu2O nanocompositeCytotoxicityAntimicrobialAntibiofilm |
| spellingShingle | Moorthy Maruthapandi Akanksha Gupta Arumugam Saravanan Gila Jacobi Ehud Banin John H.T. Luong Aharon Gedanken Ultrasonic-assisted synthesis of lignin-capped Cu2O nanocomposite with antibiofilm properties Ultrasonics Sonochemistry Ultrasonic method Lignin Cu2O nanocomposite Cytotoxicity Antimicrobial Antibiofilm |
| title | Ultrasonic-assisted synthesis of lignin-capped Cu2O nanocomposite with antibiofilm properties |
| title_full | Ultrasonic-assisted synthesis of lignin-capped Cu2O nanocomposite with antibiofilm properties |
| title_fullStr | Ultrasonic-assisted synthesis of lignin-capped Cu2O nanocomposite with antibiofilm properties |
| title_full_unstemmed | Ultrasonic-assisted synthesis of lignin-capped Cu2O nanocomposite with antibiofilm properties |
| title_short | Ultrasonic-assisted synthesis of lignin-capped Cu2O nanocomposite with antibiofilm properties |
| title_sort | ultrasonic assisted synthesis of lignin capped cu2o nanocomposite with antibiofilm properties |
| topic | Ultrasonic method Lignin Cu2O nanocomposite Cytotoxicity Antimicrobial Antibiofilm |
| url | http://www.sciencedirect.com/science/article/pii/S1350417722003376 |
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