Association of radiotherapy with preferential depletion of luminal epithelial cells in a <it>BRCA1</it> mutation carrier
<p>Abstract</p> <p>Radiation therapy (RT) after breast conservation therapy has recently been linked with significant reduction in risk of ipsilateral breast cancer among <it>BRCA1</it> mutation carriers. However, the exact mechanism by which RT reduces incidence of <...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-10-01
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| Series: | Experimental Hematology & Oncology |
| Subjects: | |
| Online Access: | http://www.ehoonline.org/content/1/1/31 |
| _version_ | 1818113581377388544 |
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| author | Chiang Huai-Chin Nair Sreejith J Yeh I-Tien Santillan Alfredo A Hu Yanfen Elledge Richard Li Rong |
| author_facet | Chiang Huai-Chin Nair Sreejith J Yeh I-Tien Santillan Alfredo A Hu Yanfen Elledge Richard Li Rong |
| author_sort | Chiang Huai-Chin |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Radiation therapy (RT) after breast conservation therapy has recently been linked with significant reduction in risk of ipsilateral breast cancer among <it>BRCA1</it> mutation carriers. However, the exact mechanism by which RT reduces incidence of <it>BRCA1</it>-associated cancer remains unclear. Here we studied fresh breast tissue from a <it>BRCA1</it> mutation carrier who was initially treated with a lumpectomy and RT for a unilateral cancer and two years later chose a prophylactic bilateral mastectomy while remaining cancer-free. Flow cytometry analysis demonstrated a strikingly lower luminal cell population in the irradiated breast as compared to the non-irradiated breast, which was confirmed by immunohistochemistry. Furthermore, the irradiated breast tissue exhibited very low progenitor cell activity <it>in vitro</it>. Given the emerging evidence that <it>BRCA1</it> tumors originate from luminal progenitor cells, our observations suggest that preferential and long-lasting elimination of luminal ductal epithelium may partly underlie the mechanism of RT-associated reduction in recurrence of <it>BRCA1</it>-associated cancer.</p> |
| first_indexed | 2024-12-11T03:37:06Z |
| format | Article |
| id | doaj.art-d4985f46de074367843fb9c3013ddb37 |
| institution | Directory Open Access Journal |
| issn | 2162-3619 |
| language | English |
| last_indexed | 2024-12-11T03:37:06Z |
| publishDate | 2012-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Experimental Hematology & Oncology |
| spelling | doaj.art-d4985f46de074367843fb9c3013ddb372022-12-22T01:22:14ZengBMCExperimental Hematology & Oncology2162-36192012-10-01113110.1186/2162-3619-1-31Association of radiotherapy with preferential depletion of luminal epithelial cells in a <it>BRCA1</it> mutation carrierChiang Huai-ChinNair Sreejith JYeh I-TienSantillan Alfredo AHu YanfenElledge RichardLi Rong<p>Abstract</p> <p>Radiation therapy (RT) after breast conservation therapy has recently been linked with significant reduction in risk of ipsilateral breast cancer among <it>BRCA1</it> mutation carriers. However, the exact mechanism by which RT reduces incidence of <it>BRCA1</it>-associated cancer remains unclear. Here we studied fresh breast tissue from a <it>BRCA1</it> mutation carrier who was initially treated with a lumpectomy and RT for a unilateral cancer and two years later chose a prophylactic bilateral mastectomy while remaining cancer-free. Flow cytometry analysis demonstrated a strikingly lower luminal cell population in the irradiated breast as compared to the non-irradiated breast, which was confirmed by immunohistochemistry. Furthermore, the irradiated breast tissue exhibited very low progenitor cell activity <it>in vitro</it>. Given the emerging evidence that <it>BRCA1</it> tumors originate from luminal progenitor cells, our observations suggest that preferential and long-lasting elimination of luminal ductal epithelium may partly underlie the mechanism of RT-associated reduction in recurrence of <it>BRCA1</it>-associated cancer.</p>http://www.ehoonline.org/content/1/1/31RadiationLuminal progenitor cellsCell of originBRCA1 |
| spellingShingle | Chiang Huai-Chin Nair Sreejith J Yeh I-Tien Santillan Alfredo A Hu Yanfen Elledge Richard Li Rong Association of radiotherapy with preferential depletion of luminal epithelial cells in a <it>BRCA1</it> mutation carrier Experimental Hematology & Oncology Radiation Luminal progenitor cells Cell of origin BRCA1 |
| title | Association of radiotherapy with preferential depletion of luminal epithelial cells in a <it>BRCA1</it> mutation carrier |
| title_full | Association of radiotherapy with preferential depletion of luminal epithelial cells in a <it>BRCA1</it> mutation carrier |
| title_fullStr | Association of radiotherapy with preferential depletion of luminal epithelial cells in a <it>BRCA1</it> mutation carrier |
| title_full_unstemmed | Association of radiotherapy with preferential depletion of luminal epithelial cells in a <it>BRCA1</it> mutation carrier |
| title_short | Association of radiotherapy with preferential depletion of luminal epithelial cells in a <it>BRCA1</it> mutation carrier |
| title_sort | association of radiotherapy with preferential depletion of luminal epithelial cells in a it brca1 it mutation carrier |
| topic | Radiation Luminal progenitor cells Cell of origin BRCA1 |
| url | http://www.ehoonline.org/content/1/1/31 |
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