Pasteurellaceae ComE1 proteins combine the properties of fibronectin adhesins and DNA binding competence proteins.
A novel fibronectin-binding protein from Pasteurella multocida (PM1665) that binds to the fibronectin type III(9-10) modules via two helix-hairpin-helix motifs has recently been described [1]. This protein shares homology with competence-related DNA-binding and uptake proteins (ComEA and ComE) from...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2601033?pdf=render |
| _version_ | 1818023323528855552 |
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| author | Lisa M Mullen Janine T Bossé Sean P Nair John M Ward Andrew N Rycroft Giles Robertson Paul R Langford Brian Henderson |
| author_facet | Lisa M Mullen Janine T Bossé Sean P Nair John M Ward Andrew N Rycroft Giles Robertson Paul R Langford Brian Henderson |
| author_sort | Lisa M Mullen |
| collection | DOAJ |
| description | A novel fibronectin-binding protein from Pasteurella multocida (PM1665) that binds to the fibronectin type III(9-10) modules via two helix-hairpin-helix motifs has recently been described [1]. This protein shares homology with competence-related DNA-binding and uptake proteins (ComEA and ComE) from Gram-positive and Gram-negative bacteria. Here, we show that recombinant PM1665 (now designated ComE1) also binds to DNA through the same helix-hairpin-helix motifs required for fibronectin-binding. This binding to DNA is non sequence-specific and is confined to double-stranded DNA. We have cloned and expressed ComE1 proteins from five members of the Pasteurellaceae in order to further investigate the function(s) of these proteins. When expressed as recombinant GST-fusion proteins, all of the homologues bound both to fibronectin and to double-stranded DNA. Inactivation of the gene encoding the ComE1 homologue in Actinobacillus pleuropneumoniae indicates major roles for these proteins in at least two processes: natural transformation, and binding of bacteria to fibronectin. |
| first_indexed | 2024-12-10T03:42:30Z |
| format | Article |
| id | doaj.art-d498d0a7236f4485b07f01f0379616b4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T03:42:30Z |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d498d0a7236f4485b07f01f0379616b42022-12-22T02:03:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01312e399110.1371/journal.pone.0003991Pasteurellaceae ComE1 proteins combine the properties of fibronectin adhesins and DNA binding competence proteins.Lisa M MullenJanine T BosséSean P NairJohn M WardAndrew N RycroftGiles RobertsonPaul R LangfordBrian HendersonA novel fibronectin-binding protein from Pasteurella multocida (PM1665) that binds to the fibronectin type III(9-10) modules via two helix-hairpin-helix motifs has recently been described [1]. This protein shares homology with competence-related DNA-binding and uptake proteins (ComEA and ComE) from Gram-positive and Gram-negative bacteria. Here, we show that recombinant PM1665 (now designated ComE1) also binds to DNA through the same helix-hairpin-helix motifs required for fibronectin-binding. This binding to DNA is non sequence-specific and is confined to double-stranded DNA. We have cloned and expressed ComE1 proteins from five members of the Pasteurellaceae in order to further investigate the function(s) of these proteins. When expressed as recombinant GST-fusion proteins, all of the homologues bound both to fibronectin and to double-stranded DNA. Inactivation of the gene encoding the ComE1 homologue in Actinobacillus pleuropneumoniae indicates major roles for these proteins in at least two processes: natural transformation, and binding of bacteria to fibronectin.http://europepmc.org/articles/PMC2601033?pdf=render |
| spellingShingle | Lisa M Mullen Janine T Bossé Sean P Nair John M Ward Andrew N Rycroft Giles Robertson Paul R Langford Brian Henderson Pasteurellaceae ComE1 proteins combine the properties of fibronectin adhesins and DNA binding competence proteins. PLoS ONE |
| title | Pasteurellaceae ComE1 proteins combine the properties of fibronectin adhesins and DNA binding competence proteins. |
| title_full | Pasteurellaceae ComE1 proteins combine the properties of fibronectin adhesins and DNA binding competence proteins. |
| title_fullStr | Pasteurellaceae ComE1 proteins combine the properties of fibronectin adhesins and DNA binding competence proteins. |
| title_full_unstemmed | Pasteurellaceae ComE1 proteins combine the properties of fibronectin adhesins and DNA binding competence proteins. |
| title_short | Pasteurellaceae ComE1 proteins combine the properties of fibronectin adhesins and DNA binding competence proteins. |
| title_sort | pasteurellaceae come1 proteins combine the properties of fibronectin adhesins and dna binding competence proteins |
| url | http://europepmc.org/articles/PMC2601033?pdf=render |
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