Development of nanoparticles incorporated with quercetin and ACE2-membrane as a novel therapy for COVID-19
Abstract Introduction Angiotensin-converting enzyme 2 (ACE2) and AXL tyrosine kinase receptor are known to be involved in the SARS-CoV-2 entry of the host cell. Therefore, targeting ACE2 and AXL should be an effective strategy to inhibit virus entry into cells. However, developing agents that can si...
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-024-02435-2 |
| _version_ | 1827284118205890560 |
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| author | Jia-You Fang Kuo-Yen Huang Tong-Hong Wang Zih-Chan Lin Chin-Chuan Chen Sui-Yuan Chang En-Li Chen Tai-Ling Chao Shuenn-Chen Yang Pan-Chyr Yang Chi-Yuan Chen |
| author_facet | Jia-You Fang Kuo-Yen Huang Tong-Hong Wang Zih-Chan Lin Chin-Chuan Chen Sui-Yuan Chang En-Li Chen Tai-Ling Chao Shuenn-Chen Yang Pan-Chyr Yang Chi-Yuan Chen |
| author_sort | Jia-You Fang |
| collection | DOAJ |
| description | Abstract Introduction Angiotensin-converting enzyme 2 (ACE2) and AXL tyrosine kinase receptor are known to be involved in the SARS-CoV-2 entry of the host cell. Therefore, targeting ACE2 and AXL should be an effective strategy to inhibit virus entry into cells. However, developing agents that can simultaneously target ACE2 and AXL remains a formidable task. The natural compound quercetin has been shown to inhibit AXL expression. Materials and methods In this study, we employed PLGA nanoparticles to prepare nanoparticles encapsulated with quercetin, coated with ACE2-containing cell membranes, or encapsulated with quercetin and then coated with ACE-2-containing cell membranes. These nanoparticles were tested for their abilities to neutralize or inhibit viral infection. Results Our data showed that nanoparticles encapsulated with quercetin and then coated with ACE2-containing cell membrane inhibited the expression of AXL without causing cytotoxic activity. Nanoparticles incorporated with both quercetin and ACE2-containing cell membrane were found to be able to neutralize pseudo virus infection and were more effective than free quercetin and nanoparticles encapsulated with quercetin at inhibition of pseudo virus and SARS-CoV-2 infection. Conclusions We have shown that the biomimetic nanoparticles incorporated with both ACE-2 membrane and quercetin showed the most antiviral activity and may be further explored for clinical application. |
| first_indexed | 2024-04-24T09:49:15Z |
| format | Article |
| id | doaj.art-d499e64a4ae440dfafb7c71ebdd6ef48 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-24T09:49:15Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-d499e64a4ae440dfafb7c71ebdd6ef482024-04-14T11:28:20ZengBMCJournal of Nanobiotechnology1477-31552024-04-0122111510.1186/s12951-024-02435-2Development of nanoparticles incorporated with quercetin and ACE2-membrane as a novel therapy for COVID-19Jia-You Fang0Kuo-Yen Huang1Tong-Hong Wang2Zih-Chan Lin3Chin-Chuan Chen4Sui-Yuan Chang5En-Li Chen6Tai-Ling Chao7Shuenn-Chen Yang8Pan-Chyr Yang9Chi-Yuan Chen10Research Center for Food and Cosmetic Safety and Research Center for Chinese Herbal Medicine, Chang Gung University of Science and TechnologyDepartment of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan UniversityGraduate Institute of Natural Products, Chang Gung UniversityChronic Diseases and Health Promotion Research Center, Chang Gung University of Science and TechnologyGraduate Institute of Natural Products, Chang Gung UniversityDepartment of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan UniversityGraduate Institute of Natural Products, Chang Gung UniversityDepartment of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan UniversityInstitute of Biomedical Sciences, Academia SinicaInstitute of Biomedical Sciences, Academia SinicaGraduate Institute of Health Industry Technology, Research Center for Food and Cosmetic Safety and Research Center for Chinese Herbal Medicine, Chang Gung University of Science and TechnologyAbstract Introduction Angiotensin-converting enzyme 2 (ACE2) and AXL tyrosine kinase receptor are known to be involved in the SARS-CoV-2 entry of the host cell. Therefore, targeting ACE2 and AXL should be an effective strategy to inhibit virus entry into cells. However, developing agents that can simultaneously target ACE2 and AXL remains a formidable task. The natural compound quercetin has been shown to inhibit AXL expression. Materials and methods In this study, we employed PLGA nanoparticles to prepare nanoparticles encapsulated with quercetin, coated with ACE2-containing cell membranes, or encapsulated with quercetin and then coated with ACE-2-containing cell membranes. These nanoparticles were tested for their abilities to neutralize or inhibit viral infection. Results Our data showed that nanoparticles encapsulated with quercetin and then coated with ACE2-containing cell membrane inhibited the expression of AXL without causing cytotoxic activity. Nanoparticles incorporated with both quercetin and ACE2-containing cell membrane were found to be able to neutralize pseudo virus infection and were more effective than free quercetin and nanoparticles encapsulated with quercetin at inhibition of pseudo virus and SARS-CoV-2 infection. Conclusions We have shown that the biomimetic nanoparticles incorporated with both ACE-2 membrane and quercetin showed the most antiviral activity and may be further explored for clinical application.https://doi.org/10.1186/s12951-024-02435-2NanoparticlesQuercetinAXLACE2COVID-19 |
| spellingShingle | Jia-You Fang Kuo-Yen Huang Tong-Hong Wang Zih-Chan Lin Chin-Chuan Chen Sui-Yuan Chang En-Li Chen Tai-Ling Chao Shuenn-Chen Yang Pan-Chyr Yang Chi-Yuan Chen Development of nanoparticles incorporated with quercetin and ACE2-membrane as a novel therapy for COVID-19 Journal of Nanobiotechnology Nanoparticles Quercetin AXL ACE2 COVID-19 |
| title | Development of nanoparticles incorporated with quercetin and ACE2-membrane as a novel therapy for COVID-19 |
| title_full | Development of nanoparticles incorporated with quercetin and ACE2-membrane as a novel therapy for COVID-19 |
| title_fullStr | Development of nanoparticles incorporated with quercetin and ACE2-membrane as a novel therapy for COVID-19 |
| title_full_unstemmed | Development of nanoparticles incorporated with quercetin and ACE2-membrane as a novel therapy for COVID-19 |
| title_short | Development of nanoparticles incorporated with quercetin and ACE2-membrane as a novel therapy for COVID-19 |
| title_sort | development of nanoparticles incorporated with quercetin and ace2 membrane as a novel therapy for covid 19 |
| topic | Nanoparticles Quercetin AXL ACE2 COVID-19 |
| url | https://doi.org/10.1186/s12951-024-02435-2 |
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