The local and circulating SOX9 as a potential biomarker for the diagnosis of primary bone cancer
Purpose: The status of the local and circulating SOX9, a master regulator of the tumor fate, and its relevance to tumor types, severity, invasion feature, response to therapy, and chemotherapy treatment were surveyed in bone cancer in the current study. Methods: The SOX9 expression level was evaluat...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-08-01
|
| Series: | Journal of Bone Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212137420300555 |
| _version_ | 1819055821371211776 |
|---|---|
| author | Ameinh Hosseini Alireza Mirzaei Vahid Salimi Khodamorad Jamshidi Pegah Babaheidarian Soudabeh Fallah Zahra Rampisheh Narges Khademian Zohreh Abdolvahabi Mehrdad Bahrabadi Mostafa Ibrahimi Fatemeh Hosami Masoumeh Tavakoli-Yaraki |
| author_facet | Ameinh Hosseini Alireza Mirzaei Vahid Salimi Khodamorad Jamshidi Pegah Babaheidarian Soudabeh Fallah Zahra Rampisheh Narges Khademian Zohreh Abdolvahabi Mehrdad Bahrabadi Mostafa Ibrahimi Fatemeh Hosami Masoumeh Tavakoli-Yaraki |
| author_sort | Ameinh Hosseini |
| collection | DOAJ |
| description | Purpose: The status of the local and circulating SOX9, a master regulator of the tumor fate, and its relevance to tumor types, severity, invasion feature, response to therapy, and chemotherapy treatment were surveyed in bone cancer in the current study. Methods: The SOX9 expression level was evaluated in tissue and peripheral blood mononuclear cells from patients with different types of malignant and benign bone tumors also tumor margin tissues using Real-Time PCR. The protein level of SOX9 was assessed using immunohistochemistry and western blot analysis. Also, the correlations of the SOX9 expression level with the patient’s clinical and pathological features were considered. Results: The remarkable overexpression of SOX9 was detected in bone tumors compared to tumor margin tissues (P < 0.0001). Malignant bone tumors revealed a higher expression of SOX9 compared to benign tumors (P < 0.0001) while osteosarcoma tumors showed higher expression levels compared to Ewing sarcoma, and chondrosarcoma. Overexpression of SOX9 was observed in high grade, metastatic, recurrent tumors also tumors with poor response to therapy. Besides, the patients under the chemotherapy treatment demonstrated higher levels of SOX9 compared to the rest of malignant tumors (P = 0.02). The simultaneous up-regulation of circulating SOX9 in the patients with bone cancer was observed compared to healthy individuals (P < 0.0001) accompanying with overexpression of SOX9 in malignant tumors compared to benign tumors (P < 0.0001). The circulating SOX9 expression was up-regulated in the patients with malignant bone tumors who receive chemotherapy treatment also patients with high grade, metastatic, recurrent tumors. The protein level of SOX9 was in line with our data on the SOX9 gene expression. Conclusion: The simultaneous overexpression of local and circulating SOX9 in bone cancer besides its positive correlation with tumor severity, malignancy, size, and chemotherapy may deserve receiving more attention in bone cancer diagnosis and therapy. |
| first_indexed | 2024-12-21T13:13:36Z |
| format | Article |
| id | doaj.art-d49b3e88e7b9415d8854df51db6bdd49 |
| institution | Directory Open Access Journal |
| issn | 2212-1374 |
| language | English |
| last_indexed | 2024-12-21T13:13:36Z |
| publishDate | 2020-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Bone Oncology |
| spelling | doaj.art-d49b3e88e7b9415d8854df51db6bdd492022-12-21T19:02:48ZengElsevierJournal of Bone Oncology2212-13742020-08-0123100300The local and circulating SOX9 as a potential biomarker for the diagnosis of primary bone cancerAmeinh Hosseini0Alireza Mirzaei1Vahid Salimi2Khodamorad Jamshidi3Pegah Babaheidarian4Soudabeh Fallah5Zahra Rampisheh6Narges Khademian7Zohreh Abdolvahabi8Mehrdad Bahrabadi9Mostafa Ibrahimi10Fatemeh Hosami11Masoumeh Tavakoli-Yaraki12Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, IranBone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, IranDepartment of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranBone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, IranDepartment of Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, IranPreventive Medicine and Public Health Research Center, Department of Community Medicine, School of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Biochemistry and Genetics, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IranBone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, IranDepartment of Clinical Biochemistry, School of Medicine, Tarbiat Modares University, Tehran, IranDepartment of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Corresponding author at: Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, P.O. Box: 1449614535, Tehran, Iran.Purpose: The status of the local and circulating SOX9, a master regulator of the tumor fate, and its relevance to tumor types, severity, invasion feature, response to therapy, and chemotherapy treatment were surveyed in bone cancer in the current study. Methods: The SOX9 expression level was evaluated in tissue and peripheral blood mononuclear cells from patients with different types of malignant and benign bone tumors also tumor margin tissues using Real-Time PCR. The protein level of SOX9 was assessed using immunohistochemistry and western blot analysis. Also, the correlations of the SOX9 expression level with the patient’s clinical and pathological features were considered. Results: The remarkable overexpression of SOX9 was detected in bone tumors compared to tumor margin tissues (P < 0.0001). Malignant bone tumors revealed a higher expression of SOX9 compared to benign tumors (P < 0.0001) while osteosarcoma tumors showed higher expression levels compared to Ewing sarcoma, and chondrosarcoma. Overexpression of SOX9 was observed in high grade, metastatic, recurrent tumors also tumors with poor response to therapy. Besides, the patients under the chemotherapy treatment demonstrated higher levels of SOX9 compared to the rest of malignant tumors (P = 0.02). The simultaneous up-regulation of circulating SOX9 in the patients with bone cancer was observed compared to healthy individuals (P < 0.0001) accompanying with overexpression of SOX9 in malignant tumors compared to benign tumors (P < 0.0001). The circulating SOX9 expression was up-regulated in the patients with malignant bone tumors who receive chemotherapy treatment also patients with high grade, metastatic, recurrent tumors. The protein level of SOX9 was in line with our data on the SOX9 gene expression. Conclusion: The simultaneous overexpression of local and circulating SOX9 in bone cancer besides its positive correlation with tumor severity, malignancy, size, and chemotherapy may deserve receiving more attention in bone cancer diagnosis and therapy.http://www.sciencedirect.com/science/article/pii/S2212137420300555Bone cancerSOX9CSC markerMalignant bone tumorsBenign bone tumors |
| spellingShingle | Ameinh Hosseini Alireza Mirzaei Vahid Salimi Khodamorad Jamshidi Pegah Babaheidarian Soudabeh Fallah Zahra Rampisheh Narges Khademian Zohreh Abdolvahabi Mehrdad Bahrabadi Mostafa Ibrahimi Fatemeh Hosami Masoumeh Tavakoli-Yaraki The local and circulating SOX9 as a potential biomarker for the diagnosis of primary bone cancer Journal of Bone Oncology Bone cancer SOX9 CSC marker Malignant bone tumors Benign bone tumors |
| title | The local and circulating SOX9 as a potential biomarker for the diagnosis of primary bone cancer |
| title_full | The local and circulating SOX9 as a potential biomarker for the diagnosis of primary bone cancer |
| title_fullStr | The local and circulating SOX9 as a potential biomarker for the diagnosis of primary bone cancer |
| title_full_unstemmed | The local and circulating SOX9 as a potential biomarker for the diagnosis of primary bone cancer |
| title_short | The local and circulating SOX9 as a potential biomarker for the diagnosis of primary bone cancer |
| title_sort | local and circulating sox9 as a potential biomarker for the diagnosis of primary bone cancer |
| topic | Bone cancer SOX9 CSC marker Malignant bone tumors Benign bone tumors |
| url | http://www.sciencedirect.com/science/article/pii/S2212137420300555 |
| work_keys_str_mv | AT ameinhhosseini thelocalandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT alirezamirzaei thelocalandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT vahidsalimi thelocalandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT khodamoradjamshidi thelocalandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT pegahbabaheidarian thelocalandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT soudabehfallah thelocalandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT zahrarampisheh thelocalandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT nargeskhademian thelocalandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT zohrehabdolvahabi thelocalandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT mehrdadbahrabadi thelocalandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT mostafaibrahimi thelocalandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT fatemehhosami thelocalandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT masoumehtavakoliyaraki thelocalandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT ameinhhosseini localandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT alirezamirzaei localandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT vahidsalimi localandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT khodamoradjamshidi localandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT pegahbabaheidarian localandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT soudabehfallah localandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT zahrarampisheh localandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT nargeskhademian localandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT zohrehabdolvahabi localandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT mehrdadbahrabadi localandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT mostafaibrahimi localandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT fatemehhosami localandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer AT masoumehtavakoliyaraki localandcirculatingsox9asapotentialbiomarkerforthediagnosisofprimarybonecancer |