Renal Senescence, Telomere Shortening and Nitrosative Stress in Feline Chronic Kidney Disease
Kidney tissues from cats with naturally occurring chronic kidney disease (CKD) and adult and senior cats without CKD were assessed to determine whether telomere shortening and nitrosative stress are associated with senescence in feline CKD. The histopathologic assessment of percent global glomerulos...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/8/12/314 |
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| author | Jessica Quimby Andrea Erickson Shannon Mcleland Rachel Cianciolo David Maranon Katharine Lunn Jonathan Elliott Jack Lawson Ann Hess Rene Paschall Susan Bailey |
| author_facet | Jessica Quimby Andrea Erickson Shannon Mcleland Rachel Cianciolo David Maranon Katharine Lunn Jonathan Elliott Jack Lawson Ann Hess Rene Paschall Susan Bailey |
| author_sort | Jessica Quimby |
| collection | DOAJ |
| description | Kidney tissues from cats with naturally occurring chronic kidney disease (CKD) and adult and senior cats without CKD were assessed to determine whether telomere shortening and nitrosative stress are associated with senescence in feline CKD. The histopathologic assessment of percent global glomerulosclerosis, inflammatory infiltrate, and fibrosis was performed. Senescence and nitrosative stress were evaluated utilizing p16 and iNOS immunohistochemistry, respectively. Renal telomere length was evaluated using telomere fluorescent in situ hybridization combined with immunohistochemistry. CKD cats were found to have significantly increased p16 staining in both the renal cortex and corticomedullary junction compared to adult and senior cats. Senior cats had significantly increased p16 staining in the corticomedullary junction compared to adult cats. p16 staining in both the renal cortex and corticomedullary junction were found to be significantly correlated with percent global glomerulosclerosis, cortical inflammatory infiltrate, and fibrosis scores. p16 staining also correlated with age in non-CKD cats. Average telomere length was significantly decreased in CKD cats compared to adult and senior cats. CKD cats had significantly increased iNOS staining compared to adult cats. Our results demonstrate increased renal senescence, telomere shortening, and nitrosative stress in feline CKD, identifying these patients as potential candidates for senolytic therapy with translational potential. |
| first_indexed | 2024-03-10T03:56:31Z |
| format | Article |
| id | doaj.art-d49e40d129114183b0764fdf151b8a01 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T03:56:31Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-d49e40d129114183b0764fdf151b8a012023-11-23T10:55:57ZengMDPI AGVeterinary Sciences2306-73812021-12-0181231410.3390/vetsci8120314Renal Senescence, Telomere Shortening and Nitrosative Stress in Feline Chronic Kidney DiseaseJessica Quimby0Andrea Erickson1Shannon Mcleland2Rachel Cianciolo3David Maranon4Katharine Lunn5Jonathan Elliott6Jack Lawson7Ann Hess8Rene Paschall9Susan Bailey10Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH 43210, USADepartment of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH 43210, USAInternational Veterinary Renal Pathology Service, Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210, USAInternational Veterinary Renal Pathology Service, Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210, USADepartment of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80521-1618, USADepartment of Clinical Sciences, North Carolina State University, Raleigh, NC 27695-0001, USADepartment of Comparative Biomedical Sciences, Royal Veterinary College, London E16 2PX, UKDepartment of Clinical Sciences and Services, Royal Veterinary College, Herts AL9 7TA, UKDepartment of Statistics, Colorado State University, Fort Collins, CO 80521-4593, USADepartment of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH 43210, USADepartment of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80521-1618, USAKidney tissues from cats with naturally occurring chronic kidney disease (CKD) and adult and senior cats without CKD were assessed to determine whether telomere shortening and nitrosative stress are associated with senescence in feline CKD. The histopathologic assessment of percent global glomerulosclerosis, inflammatory infiltrate, and fibrosis was performed. Senescence and nitrosative stress were evaluated utilizing p16 and iNOS immunohistochemistry, respectively. Renal telomere length was evaluated using telomere fluorescent in situ hybridization combined with immunohistochemistry. CKD cats were found to have significantly increased p16 staining in both the renal cortex and corticomedullary junction compared to adult and senior cats. Senior cats had significantly increased p16 staining in the corticomedullary junction compared to adult cats. p16 staining in both the renal cortex and corticomedullary junction were found to be significantly correlated with percent global glomerulosclerosis, cortical inflammatory infiltrate, and fibrosis scores. p16 staining also correlated with age in non-CKD cats. Average telomere length was significantly decreased in CKD cats compared to adult and senior cats. CKD cats had significantly increased iNOS staining compared to adult cats. Our results demonstrate increased renal senescence, telomere shortening, and nitrosative stress in feline CKD, identifying these patients as potential candidates for senolytic therapy with translational potential.https://www.mdpi.com/2306-7381/8/12/314felinecatrenal diseasesenescenceaging |
| spellingShingle | Jessica Quimby Andrea Erickson Shannon Mcleland Rachel Cianciolo David Maranon Katharine Lunn Jonathan Elliott Jack Lawson Ann Hess Rene Paschall Susan Bailey Renal Senescence, Telomere Shortening and Nitrosative Stress in Feline Chronic Kidney Disease Veterinary Sciences feline cat renal disease senescence aging |
| title | Renal Senescence, Telomere Shortening and Nitrosative Stress in Feline Chronic Kidney Disease |
| title_full | Renal Senescence, Telomere Shortening and Nitrosative Stress in Feline Chronic Kidney Disease |
| title_fullStr | Renal Senescence, Telomere Shortening and Nitrosative Stress in Feline Chronic Kidney Disease |
| title_full_unstemmed | Renal Senescence, Telomere Shortening and Nitrosative Stress in Feline Chronic Kidney Disease |
| title_short | Renal Senescence, Telomere Shortening and Nitrosative Stress in Feline Chronic Kidney Disease |
| title_sort | renal senescence telomere shortening and nitrosative stress in feline chronic kidney disease |
| topic | feline cat renal disease senescence aging |
| url | https://www.mdpi.com/2306-7381/8/12/314 |
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