Association of overexpressed MYC gene with altered PHACTR3 and E2F4 genes contributes to non-small cell lung carcinoma pathogenesis
Background: C-Myc is one of the major cellular oncogenes overexpressed in non-small cell lung carcinoma (NSCLC). Its deregulated expression is necessary but not sufficient for malignant transformation. We evaluated expression of MYC gene in NSCLC patients and its association with alterations in the...
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| Format: | Article |
| Language: | English |
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Society of Medical Biochemists of Serbia, Belgrade
2019-01-01
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| Series: | Journal of Medical Biochemistry |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2019/1452-82581902188D.pdf |
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| author | Dragoj Miodrag Banković Jasna Podolski-Renić Ana Stojković-Burić Sonja Pešić Milica Tanić Nikola Stanković Tijana |
| author_facet | Dragoj Miodrag Banković Jasna Podolski-Renić Ana Stojković-Burić Sonja Pešić Milica Tanić Nikola Stanković Tijana |
| author_sort | Dragoj Miodrag |
| collection | DOAJ |
| description | Background: C-Myc is one of the major cellular oncogenes overexpressed in non-small cell lung carcinoma (NSCLC). Its deregulated expression is necessary but not sufficient for malignant transformation. We evaluated expression of MYC gene in NSCLC patients and its association with alterations in the genes previously identified to be related to NSCLC pathogenesis, PHACt R 3 and E2F4. Methods: We analyzed M YC gene expression by qRT-PCR in 30 NSCLC patients' samples and paired normal lung tissue. M YC expression was further statistically evaluated in relation to histopathological parameters, PHACTR3 and E2F4 gene alterations and survival. Alterations in aforementioned genes were previously detected and identified based on AP-PCR profiles of paired normal and tumor DNA samples, selection of DNA bands with altered mobility in tumor samples and their characterization by the reamplification, cloning and sequencing. Results: M YC s significantly increased in NSCLC samples and its overexpression significantly associated with squamous cell carcinoma subtype. Most importantly, MYC overexpression significantly coincided with mutations in PHACTR3 and E2F4 genes, in group of all patients and in squamous cell carcinoma subtype. Moreover, patients with jointly overexpressed M YC and altered PHACTR3 or E2F4 showed trend of shorter survival. Conclusions: Overall, MYC is frequently overexpressed in NSCLC and it is associated with mutated PHACTR3 gene, as well as mutated E2F4 gene. These joint gene alterations could be considered as potential molecular markers of NSCLC and its specific subtypes. |
| first_indexed | 2024-12-19T04:26:58Z |
| format | Article |
| id | doaj.art-d49eeaf3a51c4296bb464f48af6d4df0 |
| institution | Directory Open Access Journal |
| issn | 1452-8258 1452-8266 |
| language | English |
| last_indexed | 2024-12-19T04:26:58Z |
| publishDate | 2019-01-01 |
| publisher | Society of Medical Biochemists of Serbia, Belgrade |
| record_format | Article |
| series | Journal of Medical Biochemistry |
| spelling | doaj.art-d49eeaf3a51c4296bb464f48af6d4df02022-12-21T20:36:00ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662019-01-013821881951452-82581902188DAssociation of overexpressed MYC gene with altered PHACTR3 and E2F4 genes contributes to non-small cell lung carcinoma pathogenesisDragoj Miodrag0Banković Jasna1Podolski-Renić Ana2Stojković-Burić Sonja3Pešić Milica4Tanić Nikola5https://orcid.org/0000-0001-5817-7763Stanković Tijana6University of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Neurobiology, BelgradeUniversity of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Neurobiology, BelgradeUniversity of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Neurobiology, BelgradeUniversity of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Neurobiology, BelgradeUniversity of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Neurobiology, BelgradeUniversity of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Neurobiology, BelgradeUniversity of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Neurobiology, BelgradeBackground: C-Myc is one of the major cellular oncogenes overexpressed in non-small cell lung carcinoma (NSCLC). Its deregulated expression is necessary but not sufficient for malignant transformation. We evaluated expression of MYC gene in NSCLC patients and its association with alterations in the genes previously identified to be related to NSCLC pathogenesis, PHACt R 3 and E2F4. Methods: We analyzed M YC gene expression by qRT-PCR in 30 NSCLC patients' samples and paired normal lung tissue. M YC expression was further statistically evaluated in relation to histopathological parameters, PHACTR3 and E2F4 gene alterations and survival. Alterations in aforementioned genes were previously detected and identified based on AP-PCR profiles of paired normal and tumor DNA samples, selection of DNA bands with altered mobility in tumor samples and their characterization by the reamplification, cloning and sequencing. Results: M YC s significantly increased in NSCLC samples and its overexpression significantly associated with squamous cell carcinoma subtype. Most importantly, MYC overexpression significantly coincided with mutations in PHACTR3 and E2F4 genes, in group of all patients and in squamous cell carcinoma subtype. Moreover, patients with jointly overexpressed M YC and altered PHACTR3 or E2F4 showed trend of shorter survival. Conclusions: Overall, MYC is frequently overexpressed in NSCLC and it is associated with mutated PHACTR3 gene, as well as mutated E2F4 gene. These joint gene alterations could be considered as potential molecular markers of NSCLC and its specific subtypes.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2019/1452-82581902188D.pdfe2f4mycnon-small cell lung carcinomaphactr3 |
| spellingShingle | Dragoj Miodrag Banković Jasna Podolski-Renić Ana Stojković-Burić Sonja Pešić Milica Tanić Nikola Stanković Tijana Association of overexpressed MYC gene with altered PHACTR3 and E2F4 genes contributes to non-small cell lung carcinoma pathogenesis Journal of Medical Biochemistry e2f4 myc non-small cell lung carcinoma phactr3 |
| title | Association of overexpressed MYC gene with altered PHACTR3 and E2F4 genes contributes to non-small cell lung carcinoma pathogenesis |
| title_full | Association of overexpressed MYC gene with altered PHACTR3 and E2F4 genes contributes to non-small cell lung carcinoma pathogenesis |
| title_fullStr | Association of overexpressed MYC gene with altered PHACTR3 and E2F4 genes contributes to non-small cell lung carcinoma pathogenesis |
| title_full_unstemmed | Association of overexpressed MYC gene with altered PHACTR3 and E2F4 genes contributes to non-small cell lung carcinoma pathogenesis |
| title_short | Association of overexpressed MYC gene with altered PHACTR3 and E2F4 genes contributes to non-small cell lung carcinoma pathogenesis |
| title_sort | association of overexpressed myc gene with altered phactr3 and e2f4 genes contributes to non small cell lung carcinoma pathogenesis |
| topic | e2f4 myc non-small cell lung carcinoma phactr3 |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2019/1452-82581902188D.pdf |
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