Molecular detection of Bartonella species in wild small mammals in western Yunnan Province, China
BackgroundSmall mammals serve as the main reservoir for Bartonella and as a proxy indicator of the potential risk of Bartonella transmission from nature to humans. They offer a valuable early warning for human infection. Nevertheless, geographical variations in the impact of the host on the occurren...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1301316/full |
| _version_ | 1827661450662903808 |
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| author | Yun-Yan Luo Dan Yu Hong-Ze Zhang Zheng-Xiang Liu Ru-Dan Hong Mei Hong Zhi-Qiong Ai Jun-Jie Zhu Jia-Xiang Yin |
| author_facet | Yun-Yan Luo Dan Yu Hong-Ze Zhang Zheng-Xiang Liu Ru-Dan Hong Mei Hong Zhi-Qiong Ai Jun-Jie Zhu Jia-Xiang Yin |
| author_sort | Yun-Yan Luo |
| collection | DOAJ |
| description | BackgroundSmall mammals serve as the main reservoir for Bartonella and as a proxy indicator of the potential risk of Bartonella transmission from nature to humans. They offer a valuable early warning for human infection. Nevertheless, geographical variations in the impact of the host on the occurrence of Bartonella infection are underestimated. This study was designed to investigate the infection characteristics of Bartonella and explore its species diversity in wild small mammals in western Yunnan Province, China.MethodsWild small mammals were captured from Yulong, Jianchuan, and Lianghe counties in western Yunnan Province between 2015 and 2016. Real-time quantitative PCR (qPCR) was used to detect Bartonella infection, and the Bartonella species were identified by phylogenetic analysis. The factors associated with Bartonella infection in small mammals were analyzed by the Chi-square Test.ResultsThe prevalence of Bartonella in small mammals was 47.85% (768/1605). Lianghe County had the highest Bartonella infection rate, with 56.27% of the samples tested positive, followed by a rate of 50.91% was tested in Yulong County, and 39.97% in Jianchuan County (p < 0.001). Bartonella was detected positive in a total 25 small mammal species, with infection rates ranging from 2.17% to 100%. Niviventer fulvescens had the highest Bartonella infection rate. In comparison with the dominant small mammal species, Eothenomys mileyus had the lowest Bartonella infection rate than that in Apodemus chevrieri, Rattus tanezumi, and Apodemus draco (p < 0.001). Male small mammals had a higher infection rate than females (p < 0.05). The prevalence of Bartonella in small mammals during the summer season was higher compared to the other three seasons (p < 0.001). Woodland landscape had the highest Bartonella infection rate (p < 0.001). Bartonella rochalimae, B. japonica, B. tribocorum, B. washoensis, B. sylvatica, and B. rattimassiliensis were obtained from infected small mammals.ConclusionThis study showed a high prevalence of Bartonella was detected with various Bartonella species in small mammals in Yulong, Jianchuan, and Lianghe counties of western Yunnan Province. These findings hold significant scientific clues, providing valuable reference points for further research of Bartonella natural foci in Yunnan or other analogues environments. |
| first_indexed | 2024-03-10T00:09:33Z |
| format | Article |
| id | doaj.art-d49f1aeed3b641dbacc5c915a1eeb431 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-10T00:09:33Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-d49f1aeed3b641dbacc5c915a1eeb4312023-11-23T16:02:07ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-11-011010.3389/fvets.2023.13013161301316Molecular detection of Bartonella species in wild small mammals in western Yunnan Province, ChinaYun-Yan Luo0Dan Yu1Hong-Ze Zhang2Zheng-Xiang Liu3Ru-Dan Hong4Mei Hong5Zhi-Qiong Ai6Jun-Jie Zhu7Jia-Xiang Yin8School of Public Health, Dali University, Dali, ChinaSchool of Public Health, Dali University, Dali, ChinaSchool of Public Health, Dali University, Dali, ChinaYunnan Institute of Endemic Disease Control and Prevention, Dali, ChinaSchool of Public Health, Dali University, Dali, ChinaYunnan Institute of Endemic Disease Control and Prevention, Dali, ChinaSchool of Public Health, Dali University, Dali, ChinaSchool of Public Health, Dali University, Dali, ChinaSchool of Public Health, Dali University, Dali, ChinaBackgroundSmall mammals serve as the main reservoir for Bartonella and as a proxy indicator of the potential risk of Bartonella transmission from nature to humans. They offer a valuable early warning for human infection. Nevertheless, geographical variations in the impact of the host on the occurrence of Bartonella infection are underestimated. This study was designed to investigate the infection characteristics of Bartonella and explore its species diversity in wild small mammals in western Yunnan Province, China.MethodsWild small mammals were captured from Yulong, Jianchuan, and Lianghe counties in western Yunnan Province between 2015 and 2016. Real-time quantitative PCR (qPCR) was used to detect Bartonella infection, and the Bartonella species were identified by phylogenetic analysis. The factors associated with Bartonella infection in small mammals were analyzed by the Chi-square Test.ResultsThe prevalence of Bartonella in small mammals was 47.85% (768/1605). Lianghe County had the highest Bartonella infection rate, with 56.27% of the samples tested positive, followed by a rate of 50.91% was tested in Yulong County, and 39.97% in Jianchuan County (p < 0.001). Bartonella was detected positive in a total 25 small mammal species, with infection rates ranging from 2.17% to 100%. Niviventer fulvescens had the highest Bartonella infection rate. In comparison with the dominant small mammal species, Eothenomys mileyus had the lowest Bartonella infection rate than that in Apodemus chevrieri, Rattus tanezumi, and Apodemus draco (p < 0.001). Male small mammals had a higher infection rate than females (p < 0.05). The prevalence of Bartonella in small mammals during the summer season was higher compared to the other three seasons (p < 0.001). Woodland landscape had the highest Bartonella infection rate (p < 0.001). Bartonella rochalimae, B. japonica, B. tribocorum, B. washoensis, B. sylvatica, and B. rattimassiliensis were obtained from infected small mammals.ConclusionThis study showed a high prevalence of Bartonella was detected with various Bartonella species in small mammals in Yulong, Jianchuan, and Lianghe counties of western Yunnan Province. These findings hold significant scientific clues, providing valuable reference points for further research of Bartonella natural foci in Yunnan or other analogues environments.https://www.frontiersin.org/articles/10.3389/fvets.2023.1301316/fullBartonellasmall mammalsgeographical variationsphylogenetic analysiswestern Yunnan |
| spellingShingle | Yun-Yan Luo Dan Yu Hong-Ze Zhang Zheng-Xiang Liu Ru-Dan Hong Mei Hong Zhi-Qiong Ai Jun-Jie Zhu Jia-Xiang Yin Molecular detection of Bartonella species in wild small mammals in western Yunnan Province, China Frontiers in Veterinary Science Bartonella small mammals geographical variations phylogenetic analysis western Yunnan |
| title | Molecular detection of Bartonella species in wild small mammals in western Yunnan Province, China |
| title_full | Molecular detection of Bartonella species in wild small mammals in western Yunnan Province, China |
| title_fullStr | Molecular detection of Bartonella species in wild small mammals in western Yunnan Province, China |
| title_full_unstemmed | Molecular detection of Bartonella species in wild small mammals in western Yunnan Province, China |
| title_short | Molecular detection of Bartonella species in wild small mammals in western Yunnan Province, China |
| title_sort | molecular detection of bartonella species in wild small mammals in western yunnan province china |
| topic | Bartonella small mammals geographical variations phylogenetic analysis western Yunnan |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1301316/full |
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