Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs
Tetraploid complementation is an ideal method for demonstrating the differentiation potential of pluripotent stem cells. In this study, we selected the most efficient tetraploid production method for porcine embryos and investigated whether tetraploid blastomere aggregation could enhance the quality...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.1239448/full |
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| author | Joohyeong Lee Lian Cai Lian Cai Lian Cai Mirae Kim Mirae Kim Hyerin Choi Hyerin Choi Dongjin Oh Dongjin Oh Ali Jawad Ali Jawad Eunsong Lee Sang-Hwan Hyun Sang-Hwan Hyun Sang-Hwan Hyun |
| author_facet | Joohyeong Lee Lian Cai Lian Cai Lian Cai Mirae Kim Mirae Kim Hyerin Choi Hyerin Choi Dongjin Oh Dongjin Oh Ali Jawad Ali Jawad Eunsong Lee Sang-Hwan Hyun Sang-Hwan Hyun Sang-Hwan Hyun |
| author_sort | Joohyeong Lee |
| collection | DOAJ |
| description | Tetraploid complementation is an ideal method for demonstrating the differentiation potential of pluripotent stem cells. In this study, we selected the most efficient tetraploid production method for porcine embryos and investigated whether tetraploid blastomere aggregation could enhance the quality of tetraploid embryos. Three methods were investigated to produce tetraploid embryos: First, tetraploid embryos were produced using electro-fusion of two-cell stage parthenogenetic blastomere (FUTP). Second, somatic cell was injected into the mature oocyte and fused to produce tetraploid embryos. Third, oocytes were matured with Cytochalasin B (CB) for the late 22 h of in vitro maturation to inhibit the first polar body (PB1). Following that, non-PB1 oocytes were treated with CB for 4 h after parthenogenetic activation. There was no significant difference in the blastocyst development rate and tetraploid production rate of the embryos produced through the three methods. However, FUTP-derived blastocysts had a significantly lower percentage of apoptotic cells compared to other methods. The developmental competence of embryos, expression of trophectoderm cell marker genes, and distribution of YAP1 protein were investigated in tetraploid embryos produced using the FUTP method. The FUTP method most effectively prevented apoptosis during porcine tetraploid embryo formation. Tetraploid aggregation-derived blastocysts have a high proportion of trophectoderm with increased expression of the CDX2 mRNA and high YAP1 intensity. High-quality blastocysts derived from a tetraploid embryo aggregation can serve as suitable source material for testing the differentiation potential of pluripotent stem cells for blastocyst complementation in pigs. |
| first_indexed | 2024-03-11T10:14:10Z |
| format | Article |
| id | doaj.art-d49f301dba7a47f1921097eeab774ee3 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-03-11T10:14:10Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-d49f301dba7a47f1921097eeab774ee32023-11-16T13:01:09ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2023-11-011110.3389/fcell.2023.12394481239448Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigsJoohyeong Lee0Lian Cai1Lian Cai2Lian Cai3Mirae Kim4Mirae Kim5Hyerin Choi6Hyerin Choi7Dongjin Oh8Dongjin Oh9Ali Jawad10Ali Jawad11Eunsong Lee12Sang-Hwan Hyun13Sang-Hwan Hyun14Sang-Hwan Hyun15Department of Companion Animal Industry, College of Healthcare and Biotechnology, Semyung University, Jecheon, Republic of KoreaLaboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaInstitute of Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of KoreaGraduate School of Veterinary Biosecurity and Protection, Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaInstitute of Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaInstitute of Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaInstitute of Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaInstitute of Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of KoreaCollege of Veterinary Medicine, Kangwon National University, Chuncheon, Republic of KoreaLaboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaInstitute of Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of KoreaGraduate School of Veterinary Biosecurity and Protection, Chungbuk National University, Cheongju, Republic of KoreaTetraploid complementation is an ideal method for demonstrating the differentiation potential of pluripotent stem cells. In this study, we selected the most efficient tetraploid production method for porcine embryos and investigated whether tetraploid blastomere aggregation could enhance the quality of tetraploid embryos. Three methods were investigated to produce tetraploid embryos: First, tetraploid embryos were produced using electro-fusion of two-cell stage parthenogenetic blastomere (FUTP). Second, somatic cell was injected into the mature oocyte and fused to produce tetraploid embryos. Third, oocytes were matured with Cytochalasin B (CB) for the late 22 h of in vitro maturation to inhibit the first polar body (PB1). Following that, non-PB1 oocytes were treated with CB for 4 h after parthenogenetic activation. There was no significant difference in the blastocyst development rate and tetraploid production rate of the embryos produced through the three methods. However, FUTP-derived blastocysts had a significantly lower percentage of apoptotic cells compared to other methods. The developmental competence of embryos, expression of trophectoderm cell marker genes, and distribution of YAP1 protein were investigated in tetraploid embryos produced using the FUTP method. The FUTP method most effectively prevented apoptosis during porcine tetraploid embryo formation. Tetraploid aggregation-derived blastocysts have a high proportion of trophectoderm with increased expression of the CDX2 mRNA and high YAP1 intensity. High-quality blastocysts derived from a tetraploid embryo aggregation can serve as suitable source material for testing the differentiation potential of pluripotent stem cells for blastocyst complementation in pigs.https://www.frontiersin.org/articles/10.3389/fcell.2023.1239448/fulltetraploidblastomere aggregationtrophectodermparthenogenetic activationpig |
| spellingShingle | Joohyeong Lee Lian Cai Lian Cai Lian Cai Mirae Kim Mirae Kim Hyerin Choi Hyerin Choi Dongjin Oh Dongjin Oh Ali Jawad Ali Jawad Eunsong Lee Sang-Hwan Hyun Sang-Hwan Hyun Sang-Hwan Hyun Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs Frontiers in Cell and Developmental Biology tetraploid blastomere aggregation trophectoderm parthenogenetic activation pig |
| title | Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs |
| title_full | Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs |
| title_fullStr | Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs |
| title_full_unstemmed | Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs |
| title_short | Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs |
| title_sort | tetraploid embryo aggregation produces high quality blastocysts with an increased trophectoderm in pigs |
| topic | tetraploid blastomere aggregation trophectoderm parthenogenetic activation pig |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.1239448/full |
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