Functionalization of an extended-gate field-effect transistor (EGFET) for bacteria detection
Abstract Traditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility. Therefore, new methods of measurements are necessary to improve the detection of bacteria. Well-established electrical measurement methods...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-08272-3 |
| _version_ | 1827723651025207296 |
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| author | Lea Könemund Laurie Neumann Felix Hirschberg Rebekka Biedendieck Dieter Jahn Hans-Hermann Johannes Wolfgang Kowalsky |
| author_facet | Lea Könemund Laurie Neumann Felix Hirschberg Rebekka Biedendieck Dieter Jahn Hans-Hermann Johannes Wolfgang Kowalsky |
| author_sort | Lea Könemund |
| collection | DOAJ |
| description | Abstract Traditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility. Therefore, new methods of measurements are necessary to improve the detection of bacteria. Well-established electrical measurement methods can connect high sensitive sensing systems with biological requirements. One approach is to functionalize an extended-gate field-effect transistor’s (EGFET) sensing area with modified porphyrins containing two different linkers. One linker connects the electrode surface with the porphyrin. The other linker bonds bacteria on the functional layer through a specific peptide chain. The negative charge on the surface of the cells regulates the surface potential which has an impact on the electrical behavior of the EGFET. The attendance of attached bacteria on the functionalized sensing area could successfully be detected. |
| first_indexed | 2024-03-10T22:03:43Z |
| format | Article |
| id | doaj.art-d4a0730819f34a339aebe36df8fbab80 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T22:03:43Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d4a0730819f34a339aebe36df8fbab802023-11-19T12:53:17ZengNature PortfolioScientific Reports2045-23222022-03-0112111010.1038/s41598-022-08272-3Functionalization of an extended-gate field-effect transistor (EGFET) for bacteria detectionLea Könemund0Laurie Neumann1Felix Hirschberg2Rebekka Biedendieck3Dieter Jahn4Hans-Hermann Johannes5Wolfgang Kowalsky6Institut für Hochfrequenztechnik, Technische Universität BraunschweigInstitut für Hochfrequenztechnik, Technische Universität BraunschweigInstitut für Hochfrequenztechnik, Technische Universität BraunschweigInstitute of Microbiology and Braunschweig Integrated Centre of Systems Biology (BRICS), Technische Universität BraunschweigInstitute of Microbiology and Braunschweig Integrated Centre of Systems Biology (BRICS), Technische Universität BraunschweigInstitut für Hochfrequenztechnik, Technische Universität BraunschweigInstitut für Hochfrequenztechnik, Technische Universität BraunschweigAbstract Traditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility. Therefore, new methods of measurements are necessary to improve the detection of bacteria. Well-established electrical measurement methods can connect high sensitive sensing systems with biological requirements. One approach is to functionalize an extended-gate field-effect transistor’s (EGFET) sensing area with modified porphyrins containing two different linkers. One linker connects the electrode surface with the porphyrin. The other linker bonds bacteria on the functional layer through a specific peptide chain. The negative charge on the surface of the cells regulates the surface potential which has an impact on the electrical behavior of the EGFET. The attendance of attached bacteria on the functionalized sensing area could successfully be detected.https://doi.org/10.1038/s41598-022-08272-3 |
| spellingShingle | Lea Könemund Laurie Neumann Felix Hirschberg Rebekka Biedendieck Dieter Jahn Hans-Hermann Johannes Wolfgang Kowalsky Functionalization of an extended-gate field-effect transistor (EGFET) for bacteria detection Scientific Reports |
| title | Functionalization of an extended-gate field-effect transistor (EGFET) for bacteria detection |
| title_full | Functionalization of an extended-gate field-effect transistor (EGFET) for bacteria detection |
| title_fullStr | Functionalization of an extended-gate field-effect transistor (EGFET) for bacteria detection |
| title_full_unstemmed | Functionalization of an extended-gate field-effect transistor (EGFET) for bacteria detection |
| title_short | Functionalization of an extended-gate field-effect transistor (EGFET) for bacteria detection |
| title_sort | functionalization of an extended gate field effect transistor egfet for bacteria detection |
| url | https://doi.org/10.1038/s41598-022-08272-3 |
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