Pathophysiological relevance of sputum MUC5AC and MUC5B levels in patients with mild asthma
Background: Airway mucus hypersecretion is an important pathophysiological feature of asthma. MUC5AC and MUC5B are the major secreted polymeric mucins in airways, and their compositions affect mucus properties. Despite the increasing appreciation of MUC5AC and MUC5B compositions in asthmatic airways...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Allergology International |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893021001234 |
| _version_ | 1819157735776714752 |
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| author | Tomoko Tajiri Hisako Matsumoto Makiko Jinnai Yoshihiro Kanemitsu Tadao Nagasaki Toshiyuki Iwata Hideki Inoue Hitoshi Nakaji Tsuyoshi Oguma Isao Ito Akio Niimi |
| author_facet | Tomoko Tajiri Hisako Matsumoto Makiko Jinnai Yoshihiro Kanemitsu Tadao Nagasaki Toshiyuki Iwata Hideki Inoue Hitoshi Nakaji Tsuyoshi Oguma Isao Ito Akio Niimi |
| author_sort | Tomoko Tajiri |
| collection | DOAJ |
| description | Background: Airway mucus hypersecretion is an important pathophysiological feature of asthma. MUC5AC and MUC5B are the major secreted polymeric mucins in airways, and their compositions affect mucus properties. Despite the increasing appreciation of MUC5AC and MUC5B compositions in asthmatic airways, their pathophysiological relevance remains to be fully understood in humans. Methods: In this cross-sectional study, we prospectively enrolled newly referred steroid-untreated patients with mild asthma and healthy controls. We compared induced sputum MUC5AC and MUC5B levels between patients and controls. Subsequently, we assessed the correlation between MUC5AC and MUC5B levels and clinical indices in patients. Sputum MUC5AC and MUC5B levels were measured using enzyme-linked immunosorbent assays. Results: Sputum MUC5AC and MUC5B levels were significantly higher in patients (n = 87) than in controls (n = 22) (p = 0.0002 and p = 0.006, respectively). The ratio of sputum MUC5AC to MUC5B tended to be higher in patients than in controls (p = 0.07). Sputum MUC5AC levels significantly and positively correlated with fractional exhaled nitric oxide at expiratory flow of 50 mL/s (Spearman's rho = 0.29, p = 0.006), sputum eosinophil proportion (rho = 0.34, p = 0.0013), and airway sensitivity (rho = 0.39, p = 0.0005). By contrast, sputum MUC5B levels significantly and positively correlated with airway sensitivity (rho = 0.35, p = 0.002) and negatively correlated with airway reactivity (rho = −0.33, p = 0.004). Conclusions: Sputum MUC5AC is increased by protein levels and involved in airway type 2/eosinophilic inflammation and airway hyperresponsiveness in steroid-untreated patients with mild asthma. |
| first_indexed | 2024-12-22T16:13:30Z |
| format | Article |
| id | doaj.art-d4a3a7a8c6e9476390fa9d65dfc09b86 |
| institution | Directory Open Access Journal |
| issn | 1323-8930 |
| language | English |
| last_indexed | 2024-12-22T16:13:30Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Allergology International |
| spelling | doaj.art-d4a3a7a8c6e9476390fa9d65dfc09b862022-12-21T18:20:25ZengElsevierAllergology International1323-89302022-04-01712193199Pathophysiological relevance of sputum MUC5AC and MUC5B levels in patients with mild asthmaTomoko Tajiri0Hisako Matsumoto1Makiko Jinnai2Yoshihiro Kanemitsu3Tadao Nagasaki4Toshiyuki Iwata5Hideki Inoue6Hitoshi Nakaji7Tsuyoshi Oguma8Isao Ito9Akio Niimi10Department of Respiratory Medicine, Kyoto University, Kyoto, Japan; Department of Respiratory Medicine, Allergy and Clinical Immunology, Nagoya City University School of Medical Sciences, Aichi, Japan; Corresponding author. Department of Respiratory Medicine, Allergy and Clinical Immunology, Nagoya City University School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.Department of Respiratory Medicine, Kyoto University, Kyoto, Japan; Department of Respiratory Medicine and Allergology, Kindai University Faculty of Medicine, Osaka, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, Japan; Department of Respiratory Medicine, Allergy and Clinical Immunology, Nagoya City University School of Medical Sciences, Aichi, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, Japan; Department of Respiratory Medicine, Allergy and Clinical Immunology, Nagoya City University School of Medical Sciences, Aichi, JapanBackground: Airway mucus hypersecretion is an important pathophysiological feature of asthma. MUC5AC and MUC5B are the major secreted polymeric mucins in airways, and their compositions affect mucus properties. Despite the increasing appreciation of MUC5AC and MUC5B compositions in asthmatic airways, their pathophysiological relevance remains to be fully understood in humans. Methods: In this cross-sectional study, we prospectively enrolled newly referred steroid-untreated patients with mild asthma and healthy controls. We compared induced sputum MUC5AC and MUC5B levels between patients and controls. Subsequently, we assessed the correlation between MUC5AC and MUC5B levels and clinical indices in patients. Sputum MUC5AC and MUC5B levels were measured using enzyme-linked immunosorbent assays. Results: Sputum MUC5AC and MUC5B levels were significantly higher in patients (n = 87) than in controls (n = 22) (p = 0.0002 and p = 0.006, respectively). The ratio of sputum MUC5AC to MUC5B tended to be higher in patients than in controls (p = 0.07). Sputum MUC5AC levels significantly and positively correlated with fractional exhaled nitric oxide at expiratory flow of 50 mL/s (Spearman's rho = 0.29, p = 0.006), sputum eosinophil proportion (rho = 0.34, p = 0.0013), and airway sensitivity (rho = 0.39, p = 0.0005). By contrast, sputum MUC5B levels significantly and positively correlated with airway sensitivity (rho = 0.35, p = 0.002) and negatively correlated with airway reactivity (rho = −0.33, p = 0.004). Conclusions: Sputum MUC5AC is increased by protein levels and involved in airway type 2/eosinophilic inflammation and airway hyperresponsiveness in steroid-untreated patients with mild asthma.http://www.sciencedirect.com/science/article/pii/S1323893021001234Airway hyperresponsivenessAirway hypersecretionAsthmaMUC5ACMUC5B |
| spellingShingle | Tomoko Tajiri Hisako Matsumoto Makiko Jinnai Yoshihiro Kanemitsu Tadao Nagasaki Toshiyuki Iwata Hideki Inoue Hitoshi Nakaji Tsuyoshi Oguma Isao Ito Akio Niimi Pathophysiological relevance of sputum MUC5AC and MUC5B levels in patients with mild asthma Allergology International Airway hyperresponsiveness Airway hypersecretion Asthma MUC5AC MUC5B |
| title | Pathophysiological relevance of sputum MUC5AC and MUC5B levels in patients with mild asthma |
| title_full | Pathophysiological relevance of sputum MUC5AC and MUC5B levels in patients with mild asthma |
| title_fullStr | Pathophysiological relevance of sputum MUC5AC and MUC5B levels in patients with mild asthma |
| title_full_unstemmed | Pathophysiological relevance of sputum MUC5AC and MUC5B levels in patients with mild asthma |
| title_short | Pathophysiological relevance of sputum MUC5AC and MUC5B levels in patients with mild asthma |
| title_sort | pathophysiological relevance of sputum muc5ac and muc5b levels in patients with mild asthma |
| topic | Airway hyperresponsiveness Airway hypersecretion Asthma MUC5AC MUC5B |
| url | http://www.sciencedirect.com/science/article/pii/S1323893021001234 |
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