Phenotypic Characterization and Comparative Genomic Analyses of Mycobacteriophage WIVsmall as A New Member Assigned to F1 Subcluster
In this study, we conducted the morphological observation, biological and genomic characterization, evolutionary analysis, comparative genomics description, and proteome identification of a recently isolated mycobacteriophage, WIVsmall. Morphologically, WIVsmall is classified as a member of the Siph...
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MDPI AG
2023-08-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/8/406 |
| _version_ | 1797585142026338304 |
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| author | Xinge Guo Jing Zhang Yuhan Wang Fang Zhou Qiming Li Tieshan Teng |
| author_facet | Xinge Guo Jing Zhang Yuhan Wang Fang Zhou Qiming Li Tieshan Teng |
| author_sort | Xinge Guo |
| collection | DOAJ |
| description | In this study, we conducted the morphological observation, biological and genomic characterization, evolutionary analysis, comparative genomics description, and proteome identification of a recently isolated mycobacteriophage, WIVsmall. Morphologically, WIVsmall is classified as a member of the Siphoviridae family, characterized by a flexible tail, measuring approximately 212 nm in length. The double-stranded phage genome DNA of WIVsmall spans 53,359 base pairs, and exhibits a G + C content of 61.01%. The genome of WIVsmall comprises 103 protein-coding genes, while no tRNA genes were detected. The genome annotation unveiled the presence of functional gene clusters responsible for mycobacteriophage assembly and maturation, replication, cell lysis, and functional protein synthesis. Based on the analysis of the phylogenetic tree, the genome of WIVsmall was classified as belonging to subgroup F1. A comparative genomics analysis indicated that the WIVsmall genome exhibited the highest similarity to the phage SG4, with a percentage of 64%. The single-step growth curve analysis of WIVsmall revealed a latent period of 120 min, and an outbreak period of 200 min. |
| first_indexed | 2024-03-11T00:02:47Z |
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| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T00:02:47Z |
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| spelling | doaj.art-d4a549e0cc0840a886ce7fc866f96d102023-11-19T00:41:45ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-08-014586432644810.3390/cimb45080406Phenotypic Characterization and Comparative Genomic Analyses of Mycobacteriophage WIVsmall as A New Member Assigned to F1 SubclusterXinge Guo0Jing Zhang1Yuhan Wang2Fang Zhou3Qiming Li4Tieshan Teng5Institute of Biomedical Informatics, School of Basic Medical Sciences, Henan University, Kaifeng 475004, ChinaInstitute of Biomedical Informatics, School of Basic Medical Sciences, Henan University, Kaifeng 475004, ChinaInstitute of Biomedical Informatics, School of Basic Medical Sciences, Henan University, Kaifeng 475004, ChinaInstitute of Biomedical Informatics, School of Basic Medical Sciences, Henan University, Kaifeng 475004, ChinaInstitute of Biomedical Informatics, School of Basic Medical Sciences, Henan University, Kaifeng 475004, ChinaInstitute of Biomedical Informatics, School of Basic Medical Sciences, Henan University, Kaifeng 475004, ChinaIn this study, we conducted the morphological observation, biological and genomic characterization, evolutionary analysis, comparative genomics description, and proteome identification of a recently isolated mycobacteriophage, WIVsmall. Morphologically, WIVsmall is classified as a member of the Siphoviridae family, characterized by a flexible tail, measuring approximately 212 nm in length. The double-stranded phage genome DNA of WIVsmall spans 53,359 base pairs, and exhibits a G + C content of 61.01%. The genome of WIVsmall comprises 103 protein-coding genes, while no tRNA genes were detected. The genome annotation unveiled the presence of functional gene clusters responsible for mycobacteriophage assembly and maturation, replication, cell lysis, and functional protein synthesis. Based on the analysis of the phylogenetic tree, the genome of WIVsmall was classified as belonging to subgroup F1. A comparative genomics analysis indicated that the WIVsmall genome exhibited the highest similarity to the phage SG4, with a percentage of 64%. The single-step growth curve analysis of WIVsmall revealed a latent period of 120 min, and an outbreak period of 200 min.https://www.mdpi.com/1467-3045/45/8/406mycobacteriophagemycobacteriumantibiotic-resistant bacteria |
| spellingShingle | Xinge Guo Jing Zhang Yuhan Wang Fang Zhou Qiming Li Tieshan Teng Phenotypic Characterization and Comparative Genomic Analyses of Mycobacteriophage WIVsmall as A New Member Assigned to F1 Subcluster Current Issues in Molecular Biology mycobacteriophage mycobacterium antibiotic-resistant bacteria |
| title | Phenotypic Characterization and Comparative Genomic Analyses of Mycobacteriophage WIVsmall as A New Member Assigned to F1 Subcluster |
| title_full | Phenotypic Characterization and Comparative Genomic Analyses of Mycobacteriophage WIVsmall as A New Member Assigned to F1 Subcluster |
| title_fullStr | Phenotypic Characterization and Comparative Genomic Analyses of Mycobacteriophage WIVsmall as A New Member Assigned to F1 Subcluster |
| title_full_unstemmed | Phenotypic Characterization and Comparative Genomic Analyses of Mycobacteriophage WIVsmall as A New Member Assigned to F1 Subcluster |
| title_short | Phenotypic Characterization and Comparative Genomic Analyses of Mycobacteriophage WIVsmall as A New Member Assigned to F1 Subcluster |
| title_sort | phenotypic characterization and comparative genomic analyses of mycobacteriophage wivsmall as a new member assigned to f1 subcluster |
| topic | mycobacteriophage mycobacterium antibiotic-resistant bacteria |
| url | https://www.mdpi.com/1467-3045/45/8/406 |
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