Subcellular pathways through VGluT3-expressing mouse amacrine cells provide locally tuned object-motion-selective signals in the retina
Abstract VGluT3-expressing mouse retinal amacrine cells (VG3s) respond to small-object motion and connect to multiple types of bipolar cells (inputs) and retinal ganglion cells (RGCs, outputs). Because these input and output connections are intermixed on the same dendrites, making sense of VG3 circu...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-46996-0 |
| _version_ | 1797219694813380608 |
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| author | Karl Friedrichsen Jen-Chun Hsiang Chin-I Lin Liam McCoy Katia Valkova Daniel Kerschensteiner Josh L. Morgan |
| author_facet | Karl Friedrichsen Jen-Chun Hsiang Chin-I Lin Liam McCoy Katia Valkova Daniel Kerschensteiner Josh L. Morgan |
| author_sort | Karl Friedrichsen |
| collection | DOAJ |
| description | Abstract VGluT3-expressing mouse retinal amacrine cells (VG3s) respond to small-object motion and connect to multiple types of bipolar cells (inputs) and retinal ganglion cells (RGCs, outputs). Because these input and output connections are intermixed on the same dendrites, making sense of VG3 circuitry requires comparing the distribution of synapses across their arbors to the subcellular flow of signals. Here, we combine subcellular calcium imaging and electron microscopic connectomic reconstruction to analyze how VG3s integrate and transmit visual information. VG3s receive inputs from all nearby bipolar cell types but exhibit a strong preference for the fast type 3a bipolar cells. By comparing input distributions to VG3 dendrite responses, we show that VG3 dendrites have a short functional length constant that likely depends on inhibitory shunting. This model predicts that RGCs that extend dendrites into the middle layers of the inner plexiform encounter VG3 dendrites whose responses vary according to the local bipolar cell response type. |
| first_indexed | 2024-04-24T12:37:43Z |
| format | Article |
| id | doaj.art-d4a814c4da384b0cb83a1c901b3938e3 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-24T12:37:43Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-d4a814c4da384b0cb83a1c901b3938e32024-04-07T11:24:34ZengNature PortfolioNature Communications2041-17232024-04-0115111910.1038/s41467-024-46996-0Subcellular pathways through VGluT3-expressing mouse amacrine cells provide locally tuned object-motion-selective signals in the retinaKarl Friedrichsen0Jen-Chun Hsiang1Chin-I Lin2Liam McCoy3Katia Valkova4Daniel Kerschensteiner5Josh L. Morgan6Department of Ophthalmology and Visual Sciences, Washington University in St. LouisDepartment of Ophthalmology and Visual Sciences, Washington University in St. LouisDepartment of Ophthalmology and Visual Sciences, Washington University in St. LouisDepartment of Ophthalmology and Visual Sciences, Washington University in St. LouisDepartment of Ophthalmology and Visual Sciences, Washington University in St. LouisDepartment of Ophthalmology and Visual Sciences, Washington University in St. LouisDepartment of Ophthalmology and Visual Sciences, Washington University in St. LouisAbstract VGluT3-expressing mouse retinal amacrine cells (VG3s) respond to small-object motion and connect to multiple types of bipolar cells (inputs) and retinal ganglion cells (RGCs, outputs). Because these input and output connections are intermixed on the same dendrites, making sense of VG3 circuitry requires comparing the distribution of synapses across their arbors to the subcellular flow of signals. Here, we combine subcellular calcium imaging and electron microscopic connectomic reconstruction to analyze how VG3s integrate and transmit visual information. VG3s receive inputs from all nearby bipolar cell types but exhibit a strong preference for the fast type 3a bipolar cells. By comparing input distributions to VG3 dendrite responses, we show that VG3 dendrites have a short functional length constant that likely depends on inhibitory shunting. This model predicts that RGCs that extend dendrites into the middle layers of the inner plexiform encounter VG3 dendrites whose responses vary according to the local bipolar cell response type.https://doi.org/10.1038/s41467-024-46996-0 |
| spellingShingle | Karl Friedrichsen Jen-Chun Hsiang Chin-I Lin Liam McCoy Katia Valkova Daniel Kerschensteiner Josh L. Morgan Subcellular pathways through VGluT3-expressing mouse amacrine cells provide locally tuned object-motion-selective signals in the retina Nature Communications |
| title | Subcellular pathways through VGluT3-expressing mouse amacrine cells provide locally tuned object-motion-selective signals in the retina |
| title_full | Subcellular pathways through VGluT3-expressing mouse amacrine cells provide locally tuned object-motion-selective signals in the retina |
| title_fullStr | Subcellular pathways through VGluT3-expressing mouse amacrine cells provide locally tuned object-motion-selective signals in the retina |
| title_full_unstemmed | Subcellular pathways through VGluT3-expressing mouse amacrine cells provide locally tuned object-motion-selective signals in the retina |
| title_short | Subcellular pathways through VGluT3-expressing mouse amacrine cells provide locally tuned object-motion-selective signals in the retina |
| title_sort | subcellular pathways through vglut3 expressing mouse amacrine cells provide locally tuned object motion selective signals in the retina |
| url | https://doi.org/10.1038/s41467-024-46996-0 |
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