Esophageal wound healing by aligned smooth muscle cell-laden nanofibrous patch
The esophagus exhibits peristalsis via contraction of circularly and longitudinally aligned smooth muscles, and esophageal replacement is required if there is a critical-sized wound. In this study, we proposed to reconstruct esophageal tissues using cell electrospinning (CE), an advanced technique f...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006423000248 |
| _version_ | 1827975083268767744 |
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| author | Miji Yeo Jung Won Yoon Gyu Tae Park Sung-Chan Shin Young-Cheol Song Yong-Il Cheon Byung-Joo Lee Geun Hyung Kim Jae Ho Kim |
| author_facet | Miji Yeo Jung Won Yoon Gyu Tae Park Sung-Chan Shin Young-Cheol Song Yong-Il Cheon Byung-Joo Lee Geun Hyung Kim Jae Ho Kim |
| author_sort | Miji Yeo |
| collection | DOAJ |
| description | The esophagus exhibits peristalsis via contraction of circularly and longitudinally aligned smooth muscles, and esophageal replacement is required if there is a critical-sized wound. In this study, we proposed to reconstruct esophageal tissues using cell electrospinning (CE), an advanced technique for encapsulating living cells into fibers that allows control of the direction of fiber deposition. After treatment with transforming growth factor-β, mesenchymal stem cell-derived smooth muscle cells (SMCs) were utilized for cell electrospinning or three-dimensional bioprinting to compare the effects of aligned micropatterns on cell morphology. CE resulted in SMCs with uniaxially arranged and elongated cell morphology with upregulated expression levels of SMC-specific markers, including connexin 43, smooth muscle protein 22 alpha (SM22α), desmin, and smoothelin. When SMC-laden nanofibrous patches were transplanted into a rat esophageal defect model, the SMC patch promoted regeneration of esophageal wounds with an increased number of newly formed blood vessels and enhanced the SMC-specific markers of SM22α and vimentin. Taken together, CE with its advantages, such as guidance of highly elongated, aligned cell morphology and accelerated SMC differentiation, can be an efficient strategy to reconstruct smooth muscle tissues and treat esophageal perforation. |
| first_indexed | 2024-04-09T20:04:44Z |
| format | Article |
| id | doaj.art-d4aab4678eb24e9bb33fc8f4411299a3 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-09T20:04:44Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-d4aab4678eb24e9bb33fc8f4411299a32023-04-02T06:14:35ZengElsevierMaterials Today Bio2590-00642023-04-0119100564Esophageal wound healing by aligned smooth muscle cell-laden nanofibrous patchMiji Yeo0Jung Won Yoon1Gyu Tae Park2Sung-Chan Shin3Young-Cheol Song4Yong-Il Cheon5Byung-Joo Lee6Geun Hyung Kim7Jae Ho Kim8Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon 16419, Republic of Korea; Department of Engineering Science and Mechanics, Penn State University, University Park, PA 16802, USADepartment of Physiology, School of Medicine, Pusan National University, Yangsan 50612, Republic of KoreaDepartment of Physiology, School of Medicine, Pusan National University, Yangsan 50612, Republic of KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Pusan National University and Medical Research Institute, Pusan National University Hospital, Busan 49241, Republic of KoreaDepartment of Physiology, School of Medicine, Pusan National University, Yangsan 50612, Republic of KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Pusan National University and Medical Research Institute, Pusan National University Hospital, Busan 49241, Republic of KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Pusan National University and Medical Research Institute, Pusan National University Hospital, Busan 49241, Republic of KoreaDepartment of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon 16419, Republic of Korea; Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon 16419, Republic of Korea; Corresponding author. Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of Korea.Department of Physiology, School of Medicine, Pusan National University, Yangsan 50612, Republic of Korea; Corresponding author.The esophagus exhibits peristalsis via contraction of circularly and longitudinally aligned smooth muscles, and esophageal replacement is required if there is a critical-sized wound. In this study, we proposed to reconstruct esophageal tissues using cell electrospinning (CE), an advanced technique for encapsulating living cells into fibers that allows control of the direction of fiber deposition. After treatment with transforming growth factor-β, mesenchymal stem cell-derived smooth muscle cells (SMCs) were utilized for cell electrospinning or three-dimensional bioprinting to compare the effects of aligned micropatterns on cell morphology. CE resulted in SMCs with uniaxially arranged and elongated cell morphology with upregulated expression levels of SMC-specific markers, including connexin 43, smooth muscle protein 22 alpha (SM22α), desmin, and smoothelin. When SMC-laden nanofibrous patches were transplanted into a rat esophageal defect model, the SMC patch promoted regeneration of esophageal wounds with an increased number of newly formed blood vessels and enhanced the SMC-specific markers of SM22α and vimentin. Taken together, CE with its advantages, such as guidance of highly elongated, aligned cell morphology and accelerated SMC differentiation, can be an efficient strategy to reconstruct smooth muscle tissues and treat esophageal perforation.http://www.sciencedirect.com/science/article/pii/S2590006423000248Cell electrospinningSmooth muscle cellsEsophageal reconstruction |
| spellingShingle | Miji Yeo Jung Won Yoon Gyu Tae Park Sung-Chan Shin Young-Cheol Song Yong-Il Cheon Byung-Joo Lee Geun Hyung Kim Jae Ho Kim Esophageal wound healing by aligned smooth muscle cell-laden nanofibrous patch Materials Today Bio Cell electrospinning Smooth muscle cells Esophageal reconstruction |
| title | Esophageal wound healing by aligned smooth muscle cell-laden nanofibrous patch |
| title_full | Esophageal wound healing by aligned smooth muscle cell-laden nanofibrous patch |
| title_fullStr | Esophageal wound healing by aligned smooth muscle cell-laden nanofibrous patch |
| title_full_unstemmed | Esophageal wound healing by aligned smooth muscle cell-laden nanofibrous patch |
| title_short | Esophageal wound healing by aligned smooth muscle cell-laden nanofibrous patch |
| title_sort | esophageal wound healing by aligned smooth muscle cell laden nanofibrous patch |
| topic | Cell electrospinning Smooth muscle cells Esophageal reconstruction |
| url | http://www.sciencedirect.com/science/article/pii/S2590006423000248 |
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