Screening of Strongyloides infection using an ELISA test in transplant candidates
OBJECTIVES: Hyperinfection or disseminated strongyloidiasis has been frequently reported after transplants and is related to high mortality. This study aimed to screen for strongyloidiasis using serological diagnoses in transplant candidates. METHODS: An ELISA test was performed with filariform lar...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier España
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| Series: | Clinics |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322019000100235&lng=en&tlng=en |
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| author | Beatriz Toledo Marcelo A. Corral Dirce Mary C.L. Meisel Maiara Gottardi Edson Abdala Silvia F. Costa Lígia Camera Pierrotti Susana A.Z. Lescano Elenice M.N. Gonçalves Vera L.P. Castilho Pedro P. Chieffi Ronaldo C.B. Gryschek Fabiana M. Paula |
| author_facet | Beatriz Toledo Marcelo A. Corral Dirce Mary C.L. Meisel Maiara Gottardi Edson Abdala Silvia F. Costa Lígia Camera Pierrotti Susana A.Z. Lescano Elenice M.N. Gonçalves Vera L.P. Castilho Pedro P. Chieffi Ronaldo C.B. Gryschek Fabiana M. Paula |
| author_sort | Beatriz Toledo |
| collection | DOAJ |
| description | OBJECTIVES: Hyperinfection or disseminated strongyloidiasis has been frequently reported after transplants and is related to high mortality. This study aimed to screen for strongyloidiasis using serological diagnoses in transplant candidates. METHODS: An ELISA test was performed with filariform larvae of Strongyloides venezuelensis as a source of antigen. RESULTS: In the serum from transplant candidates, anti-Strongyloides IgG antibodies were detected in 35/150 (23.3%) samples by soluble fractions in phosphate buffered saline (PBS), 31/150 (20.7%) samples by soluble fractions in Tris-HCl, 27/150 (18.0%) samples by membrane fractions in PBS and 22/150 (14.7%) samples by membrane fractions in Tris-HCl. CONCLUSIONS: The present results suggest the ELISA test, ideally using soluble fractions of filariform larvae S. venezuelensis in PBS, as an additional strategy for the diagnosis of strongyloidiasis in transplant candidates. |
| first_indexed | 2024-12-12T15:09:45Z |
| format | Article |
| id | doaj.art-d4aae3188b7b41ab9621a378a24af8d4 |
| institution | Directory Open Access Journal |
| issn | 1980-5322 |
| language | English |
| last_indexed | 2024-12-12T15:09:45Z |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-d4aae3188b7b41ab9621a378a24af8d42022-12-22T00:20:39ZengElsevier EspañaClinics1980-532274010.6061/clinics/2019/e698S1807-59322019000100235Screening of Strongyloides infection using an ELISA test in transplant candidatesBeatriz ToledoMarcelo A. CorralDirce Mary C.L. MeiselMaiara GottardiEdson AbdalaSilvia F. CostaLígia Camera PierrottiSusana A.Z. LescanoElenice M.N. GonçalvesVera L.P. CastilhoPedro P. ChieffiRonaldo C.B. GryschekFabiana M. PaulaOBJECTIVES: Hyperinfection or disseminated strongyloidiasis has been frequently reported after transplants and is related to high mortality. This study aimed to screen for strongyloidiasis using serological diagnoses in transplant candidates. METHODS: An ELISA test was performed with filariform larvae of Strongyloides venezuelensis as a source of antigen. RESULTS: In the serum from transplant candidates, anti-Strongyloides IgG antibodies were detected in 35/150 (23.3%) samples by soluble fractions in phosphate buffered saline (PBS), 31/150 (20.7%) samples by soluble fractions in Tris-HCl, 27/150 (18.0%) samples by membrane fractions in PBS and 22/150 (14.7%) samples by membrane fractions in Tris-HCl. CONCLUSIONS: The present results suggest the ELISA test, ideally using soluble fractions of filariform larvae S. venezuelensis in PBS, as an additional strategy for the diagnosis of strongyloidiasis in transplant candidates.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322019000100235&lng=en&tlng=enHeterologous AntigenStrongyloides VenezuelensisELISA TestTransplant Candidates |
| spellingShingle | Beatriz Toledo Marcelo A. Corral Dirce Mary C.L. Meisel Maiara Gottardi Edson Abdala Silvia F. Costa Lígia Camera Pierrotti Susana A.Z. Lescano Elenice M.N. Gonçalves Vera L.P. Castilho Pedro P. Chieffi Ronaldo C.B. Gryschek Fabiana M. Paula Screening of Strongyloides infection using an ELISA test in transplant candidates Clinics Heterologous Antigen Strongyloides Venezuelensis ELISA Test Transplant Candidates |
| title | Screening of Strongyloides infection using an ELISA test in transplant candidates |
| title_full | Screening of Strongyloides infection using an ELISA test in transplant candidates |
| title_fullStr | Screening of Strongyloides infection using an ELISA test in transplant candidates |
| title_full_unstemmed | Screening of Strongyloides infection using an ELISA test in transplant candidates |
| title_short | Screening of Strongyloides infection using an ELISA test in transplant candidates |
| title_sort | screening of strongyloides infection using an elisa test in transplant candidates |
| topic | Heterologous Antigen Strongyloides Venezuelensis ELISA Test Transplant Candidates |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322019000100235&lng=en&tlng=en |
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