Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds
Actinobacteria are among the most prolific producers of bioactive secondary metabolites. In order to collect Arctic marine bacteria for the discovery of new bioactive metabolites, actinobacteria were selectively isolated during a research cruise in the Greenland Sea, Norwegian Sea and the Barents Se...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1005625/full |
| _version_ | 1811317969126424576 |
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| author | Yannik K. Schneider Ole C. Hagestad Chun Li Espen H. Hansen Jeanette H. Andersen |
| author_facet | Yannik K. Schneider Ole C. Hagestad Chun Li Espen H. Hansen Jeanette H. Andersen |
| author_sort | Yannik K. Schneider |
| collection | DOAJ |
| description | Actinobacteria are among the most prolific producers of bioactive secondary metabolites. In order to collect Arctic marine bacteria for the discovery of new bioactive metabolites, actinobacteria were selectively isolated during a research cruise in the Greenland Sea, Norwegian Sea and the Barents Sea. In the frame of the isolation campaign, it was investigated how different sample treatments, isolation media and sample-sources, such as animals and sediments, affected the yield of actinobacterial isolates to aid further isolation campaigns. Special attention was given to sediments, where we expected spores of spore forming bacteria to enrich. Beside actinobacteria a high share of bacilli was obtained which was not desired. An experimental protocol for down-scaled cultivation and extraction was tested and compared with an established low-throughput cultivation and extraction protocol. The heat-shock method proved suitable to enrich spore-, or endospore forming bacteria such as bacilli. Finally, a group bioactive compounds could be tentatively identified using UHPLC–MS/MS analysis of the active fractions. |
| first_indexed | 2024-04-13T12:17:39Z |
| format | Article |
| id | doaj.art-d4b2f8ba4bf14e89835518e528ada5c9 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T12:17:39Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d4b2f8ba4bf14e89835518e528ada5c92022-12-22T02:47:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-11-011310.3389/fmicb.2022.10056251005625Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compoundsYannik K. Schneider0Ole C. Hagestad1Chun Li2Espen H. Hansen3Jeanette H. Andersen4Marbio, Faculty for Fisheries, Biosciences and Economy, UiT—The Arctic University of Norway, Tromsø, NorwayMarbank, Institute of Marine Research, Tromsø, NorwayMarbio, Faculty for Fisheries, Biosciences and Economy, UiT—The Arctic University of Norway, Tromsø, NorwayMarbio, Faculty for Fisheries, Biosciences and Economy, UiT—The Arctic University of Norway, Tromsø, NorwayMarbio, Faculty for Fisheries, Biosciences and Economy, UiT—The Arctic University of Norway, Tromsø, NorwayActinobacteria are among the most prolific producers of bioactive secondary metabolites. In order to collect Arctic marine bacteria for the discovery of new bioactive metabolites, actinobacteria were selectively isolated during a research cruise in the Greenland Sea, Norwegian Sea and the Barents Sea. In the frame of the isolation campaign, it was investigated how different sample treatments, isolation media and sample-sources, such as animals and sediments, affected the yield of actinobacterial isolates to aid further isolation campaigns. Special attention was given to sediments, where we expected spores of spore forming bacteria to enrich. Beside actinobacteria a high share of bacilli was obtained which was not desired. An experimental protocol for down-scaled cultivation and extraction was tested and compared with an established low-throughput cultivation and extraction protocol. The heat-shock method proved suitable to enrich spore-, or endospore forming bacteria such as bacilli. Finally, a group bioactive compounds could be tentatively identified using UHPLC–MS/MS analysis of the active fractions.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1005625/fullactinobacteriaisolationnatural productsbioprospectingmarineArctic |
| spellingShingle | Yannik K. Schneider Ole C. Hagestad Chun Li Espen H. Hansen Jeanette H. Andersen Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds Frontiers in Microbiology actinobacteria isolation natural products bioprospecting marine Arctic |
| title | Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
| title_full | Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
| title_fullStr | Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
| title_full_unstemmed | Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
| title_short | Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
| title_sort | selective isolation of arctic marine actinobacteria and a down scaled fermentation and extraction strategy for identifying bioactive compounds |
| topic | actinobacteria isolation natural products bioprospecting marine Arctic |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1005625/full |
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