Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium.
Differentiation is an epigenetic program that involves the gradual loss of pluripotency and acquisition of cell type-specific features. Understanding these processes requires genome-wide analysis of epigenetic and gene expression profiles, which have been challenging in primary tissue samples due to...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-04-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3080862?pdf=render |
| _version_ | 1818925271261118464 |
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| author | Reo Maruyama Sibgat Choudhury Adam Kowalczyk Marina Bessarabova Bryan Beresford-Smith Thomas Conway Antony Kaspi Zhenhua Wu Tatiana Nikolskaya Vanessa F Merino Pang-Kuo Lo X Shirley Liu Yuri Nikolsky Saraswati Sukumar Izhak Haviv Kornelia Polyak |
| author_facet | Reo Maruyama Sibgat Choudhury Adam Kowalczyk Marina Bessarabova Bryan Beresford-Smith Thomas Conway Antony Kaspi Zhenhua Wu Tatiana Nikolskaya Vanessa F Merino Pang-Kuo Lo X Shirley Liu Yuri Nikolsky Saraswati Sukumar Izhak Haviv Kornelia Polyak |
| author_sort | Reo Maruyama |
| collection | DOAJ |
| description | Differentiation is an epigenetic program that involves the gradual loss of pluripotency and acquisition of cell type-specific features. Understanding these processes requires genome-wide analysis of epigenetic and gene expression profiles, which have been challenging in primary tissue samples due to limited numbers of cells available. Here we describe the application of high-throughput sequencing technology for profiling histone and DNA methylation, as well as gene expression patterns of normal human mammary progenitor-enriched and luminal lineage-committed cells. We observed significant differences in histone H3 lysine 27 tri-methylation (H3K27me3) enrichment and DNA methylation of genes expressed in a cell type-specific manner, suggesting their regulation by epigenetic mechanisms and a dynamic interplay between the two processes that together define developmental potential. The technologies we developed and the epigenetically regulated genes we identified will accelerate the characterization of primary cell epigenomes and the dissection of human mammary epithelial lineage-commitment and luminal differentiation. |
| first_indexed | 2024-12-20T02:38:34Z |
| format | Article |
| id | doaj.art-d4b3642e06ac4e1f994d1ffc664b3e25 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-20T02:38:34Z |
| publishDate | 2011-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-d4b3642e06ac4e1f994d1ffc664b3e252022-12-21T19:56:23ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042011-04-0174e100136910.1371/journal.pgen.1001369Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium.Reo MaruyamaSibgat ChoudhuryAdam KowalczykMarina BessarabovaBryan Beresford-SmithThomas ConwayAntony KaspiZhenhua WuTatiana NikolskayaVanessa F MerinoPang-Kuo LoX Shirley LiuYuri NikolskySaraswati SukumarIzhak HavivKornelia PolyakDifferentiation is an epigenetic program that involves the gradual loss of pluripotency and acquisition of cell type-specific features. Understanding these processes requires genome-wide analysis of epigenetic and gene expression profiles, which have been challenging in primary tissue samples due to limited numbers of cells available. Here we describe the application of high-throughput sequencing technology for profiling histone and DNA methylation, as well as gene expression patterns of normal human mammary progenitor-enriched and luminal lineage-committed cells. We observed significant differences in histone H3 lysine 27 tri-methylation (H3K27me3) enrichment and DNA methylation of genes expressed in a cell type-specific manner, suggesting their regulation by epigenetic mechanisms and a dynamic interplay between the two processes that together define developmental potential. The technologies we developed and the epigenetically regulated genes we identified will accelerate the characterization of primary cell epigenomes and the dissection of human mammary epithelial lineage-commitment and luminal differentiation.http://europepmc.org/articles/PMC3080862?pdf=render |
| spellingShingle | Reo Maruyama Sibgat Choudhury Adam Kowalczyk Marina Bessarabova Bryan Beresford-Smith Thomas Conway Antony Kaspi Zhenhua Wu Tatiana Nikolskaya Vanessa F Merino Pang-Kuo Lo X Shirley Liu Yuri Nikolsky Saraswati Sukumar Izhak Haviv Kornelia Polyak Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium. PLoS Genetics |
| title | Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium. |
| title_full | Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium. |
| title_fullStr | Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium. |
| title_full_unstemmed | Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium. |
| title_short | Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium. |
| title_sort | epigenetic regulation of cell type specific expression patterns in the human mammary epithelium |
| url | http://europepmc.org/articles/PMC3080862?pdf=render |
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