Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens

Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens

The adaptation of CRISPR/SpCas9 technology to mammalian cell lines is transforming the study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs) targeting human protein-coding genes and encoded in viral vectors have been used to systematically create gene knockouts in a varie...

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Bibliographic Details
Main Authors: Traver Hart, Amy Hin Yan Tong, Katie Chan, Jolanda Van Leeuwen, Ashwin Seetharaman, Michael Aregger, Megha Chandrashekhar, Nicole Hustedt, Sahil Seth, Avery Noonan, Andrea Habsid, Olga Sizova, Lyudmila Nedyalkova, Ryan Climie, Leanne Tworzyanski, Keith Lawson, Maria Augusta Sartori, Sabriyeh Alibeh, David Tieu, Sanna Masud, Patricia Mero, Alexander Weiss, Kevin R. Brown, Matej Usaj, Maximilian Billmann, Mahfuzur Rahman, Michael Constanzo, Chad L. Myers, Brenda J. Andrews, Charles Boone, Daniel Durocher, Jason Moffat
Format: Article
Language:English
Published: Oxford University Press 2017-08-01
Series:G3: Genes, Genomes, Genetics
Subjects:
genetic screens
CRISPR/Cas9
core essential genes
cancer cell lines
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.117.041277

Internet

http://g3journal.org/lookup/doi/10.1534/g3.117.041277

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