Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches
Abstract Background Wickerhamomyces anomalus (W. anomalus) is a kind of non-Saccharomyces yeast that has a variety of unique physiological characteristics and metabolic features and is widely used in many fields, such as food preservation, biomass energy, and aquaculture feed protein production. How...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-022-02691-y |
| _version_ | 1811309268904706048 |
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| author | Yinfeng Li Hua Long Guilan Jiang Xun Gong Zhihai Yu Mingzheng Huang Tianbing Guan Yuanyuan Guan Xiaozhu Liu |
| author_facet | Yinfeng Li Hua Long Guilan Jiang Xun Gong Zhihai Yu Mingzheng Huang Tianbing Guan Yuanyuan Guan Xiaozhu Liu |
| author_sort | Yinfeng Li |
| collection | DOAJ |
| description | Abstract Background Wickerhamomyces anomalus (W. anomalus) is a kind of non-Saccharomyces yeast that has a variety of unique physiological characteristics and metabolic features and is widely used in many fields, such as food preservation, biomass energy, and aquaculture feed protein production. However, the mechanism of W. anomalus response to ethanol stress is still unclear, which greatly limits its application in the production of ethanol beverages and ethanol fuels. Therefore, we checked the effects of ethanol stress on the morphology, the growth, and differentially expressed genes (DEGs) and metabolites (DEMs) of W. anomalus. Results High concentrations of ethanol (9% ethanol and 12% ethanol) remarkably inhibited the growth of W. anomalus. Energy metabolism, amino acid metabolism, fatty acids metabolism, and nucleic acid metabolism were significantly influenced when exposing to 9% ethanol and 12% ethanolstress, which maybe universal for W. anomalus to response to different concentrations of ethanol stressl Furthermore, extracellular addition of aspartate, glutamate, and arginine significantly abated ethanol damage and improved the survival rate of W. anomalus. Conclusions The results obtained in this study provide insights into the mechanisms involved in W. anomalus response to ethanol stress. Therefore, new strategies can be realized to improve the ethanol tolerance of W. anomalus through metabolic engineering. |
| first_indexed | 2024-04-13T09:39:20Z |
| format | Article |
| id | doaj.art-d4b4f7afd8ae4ddd8e4a478f11bea954 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-13T09:39:20Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-d4b4f7afd8ae4ddd8e4a478f11bea9542022-12-22T02:52:00ZengBMCBMC Microbiology1471-21802022-11-0122111310.1186/s12866-022-02691-yAnalysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approachesYinfeng Li0Hua Long1Guilan Jiang2Xun Gong3Zhihai Yu4Mingzheng Huang5Tianbing Guan6Yuanyuan Guan7Xiaozhu Liu8Guizhou Institute of TechnologyGuizhou Institute of TechnologyGuizhou Institute of TechnologyGuizhou Institute of TechnologyGuizhou Institute of TechnologyGuizhou Institute of TechnologyChongqing University of Science and TechnologyHenan Institute of Science and TechnologyGuizhou Institute of TechnologyAbstract Background Wickerhamomyces anomalus (W. anomalus) is a kind of non-Saccharomyces yeast that has a variety of unique physiological characteristics and metabolic features and is widely used in many fields, such as food preservation, biomass energy, and aquaculture feed protein production. However, the mechanism of W. anomalus response to ethanol stress is still unclear, which greatly limits its application in the production of ethanol beverages and ethanol fuels. Therefore, we checked the effects of ethanol stress on the morphology, the growth, and differentially expressed genes (DEGs) and metabolites (DEMs) of W. anomalus. Results High concentrations of ethanol (9% ethanol and 12% ethanol) remarkably inhibited the growth of W. anomalus. Energy metabolism, amino acid metabolism, fatty acids metabolism, and nucleic acid metabolism were significantly influenced when exposing to 9% ethanol and 12% ethanolstress, which maybe universal for W. anomalus to response to different concentrations of ethanol stressl Furthermore, extracellular addition of aspartate, glutamate, and arginine significantly abated ethanol damage and improved the survival rate of W. anomalus. Conclusions The results obtained in this study provide insights into the mechanisms involved in W. anomalus response to ethanol stress. Therefore, new strategies can be realized to improve the ethanol tolerance of W. anomalus through metabolic engineering.https://doi.org/10.1186/s12866-022-02691-yEthanol stressGrowthW. anomalusTranscriptomicsMetabolomics |
| spellingShingle | Yinfeng Li Hua Long Guilan Jiang Xun Gong Zhihai Yu Mingzheng Huang Tianbing Guan Yuanyuan Guan Xiaozhu Liu Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches BMC Microbiology Ethanol stress Growth W. anomalus Transcriptomics Metabolomics |
| title | Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches |
| title_full | Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches |
| title_fullStr | Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches |
| title_full_unstemmed | Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches |
| title_short | Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches |
| title_sort | analysis of the ethanol stress response mechanism in wickerhamomyces anomalus based on transcriptomics and metabolomics approaches |
| topic | Ethanol stress Growth W. anomalus Transcriptomics Metabolomics |
| url | https://doi.org/10.1186/s12866-022-02691-y |
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