Expression and functional analyses of the mitogen-activated protein kinase (MPK) cascade genes in response to phytohormones in wheat (Triticum aestivum L.)
Mitogen-activated protein kinase (MPK) cascades consist of a set of kinase types (MPKKKs, MPKKs, MPKs) to establish conserved signal-transducing modules mediating plant growth, development as well as responses to internal and external cues. In this study, the expression patterns of six MPKKK, two MP...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-01-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311916613679 |
| _version_ | 1818643909035687936 |
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| author | Su-fei YAO Yan-xia WANG Tong-ren YANG Lin HAO Wen-jing LU Kai XIAO |
| author_facet | Su-fei YAO Yan-xia WANG Tong-ren YANG Lin HAO Wen-jing LU Kai XIAO |
| author_sort | Su-fei YAO |
| collection | DOAJ |
| description | Mitogen-activated protein kinase (MPK) cascades consist of a set of kinase types (MPKKKs, MPKKs, MPKs) to establish conserved signal-transducing modules mediating plant growth, development as well as responses to internal and external cues. In this study, the expression patterns of six MPKKK, two MPKK, and 11 MPK genes in wheat in responses to external treatments of phytohormones, including naphthylacetic acid (NAA), abscisic acid (ABA), 6-benzyladenine (6-BA), gibberellin (GA3), salisylic acid (SA), jasmonic acid (JA), and ethylene (ETH), were investigated. Expression analysis revealed that several of the MPK cascade genes are responses to the external phytohormone signaling. Of which, TaMPKKKA;3 is induced by 6-BA and NAA while TaMPK4 repressed by ETH, GA3, SA, and JA; TaMPKKKA, TaMPKKKA;3 and TaMPK1 are down-regulated by ETH and GA3 whereas TaMPK9 and TaMPK12 repressed by ETH and JA in addition that TaMPK12 also repressed by GA3; TaMPK12;1 is down-regulated by ABA, GA3 and SA while TaMPK17 repressed by all exogenous phytonormones examined. TaMPK4, a MPK type gene previously characterized to mediate tolerance to phosphate (Pi) deprivation, was functionally evaluated for its role in mediation of responses of plants to exogenous GA3, ETH, SA, and JA. Results indicated that overexpression and antisense expression of TaMPK4 in tobacco dramatically modify the growth of seedlings upon treatments of GA3, SA and JA, in which the overexpressors behaved deteriorated growth feature whereas the seedlings with antisense expression of TaMPK4 exhibited improved seedling phenotype. The growth behaviors in lines overexpressing or antisensely expressing TaMPK4 are closely associated with the biomass and the corresponding hormone-associated parameters. These results demonstrated that TaMPK4 acts as a critical player in mediating the phytohormone signaling. Our findings have identified the phytohormone-responsive MPK cascade genes in wheat and provided a connection between the phytohormone-mediated responses and the MPK cascade pathways. |
| first_indexed | 2024-12-17T00:06:26Z |
| format | Article |
| id | doaj.art-d4b89c1918094e85b26851f6f8dbc3d9 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-17T00:06:26Z |
| publishDate | 2017-01-01 |
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| series | Journal of Integrative Agriculture |
| spelling | doaj.art-d4b89c1918094e85b26851f6f8dbc3d92022-12-21T22:10:57ZengElsevierJournal of Integrative Agriculture2095-31192017-01-011612735Expression and functional analyses of the mitogen-activated protein kinase (MPK) cascade genes in response to phytohormones in wheat (Triticum aestivum L.)Su-fei YAO0Yan-xia WANG1Tong-ren YANG2Lin HAO3Wen-jing LU4Kai XIAO5College of Life Sciences, Agricultural University of Hebei, Baoding 071001, P.R.ChinaShijiazhuang Academy of Agriculture and Forestry Sciences, Shijiazhuang 050041, P.R.ChinaCollege of Life Sciences, Agricultural University of Hebei, Baoding 071001, P.R.ChinaCollege of Agronomy, Agricultural University of Hebei, Baoding 071001, P.R.China; College of Life Sciences, Agricultural University of Hebei, Baoding 071001, P.R.ChinaCollege of Life Sciences, Agricultural University of Hebei, Baoding 071001, P.R.China; LU Wen-jing, Tel: +86-312-7528246, Fax: +86-312-7528200College of Agronomy, Agricultural University of Hebei, Baoding 071001, P.R.China; Correspondence XIAO Kai, Tel: +86-312-7528115, Fax: +86-312-7528400Mitogen-activated protein kinase (MPK) cascades consist of a set of kinase types (MPKKKs, MPKKs, MPKs) to establish conserved signal-transducing modules mediating plant growth, development as well as responses to internal and external cues. In this study, the expression patterns of six MPKKK, two MPKK, and 11 MPK genes in wheat in responses to external treatments of phytohormones, including naphthylacetic acid (NAA), abscisic acid (ABA), 6-benzyladenine (6-BA), gibberellin (GA3), salisylic acid (SA), jasmonic acid (JA), and ethylene (ETH), were investigated. Expression analysis revealed that several of the MPK cascade genes are responses to the external phytohormone signaling. Of which, TaMPKKKA;3 is induced by 6-BA and NAA while TaMPK4 repressed by ETH, GA3, SA, and JA; TaMPKKKA, TaMPKKKA;3 and TaMPK1 are down-regulated by ETH and GA3 whereas TaMPK9 and TaMPK12 repressed by ETH and JA in addition that TaMPK12 also repressed by GA3; TaMPK12;1 is down-regulated by ABA, GA3 and SA while TaMPK17 repressed by all exogenous phytonormones examined. TaMPK4, a MPK type gene previously characterized to mediate tolerance to phosphate (Pi) deprivation, was functionally evaluated for its role in mediation of responses of plants to exogenous GA3, ETH, SA, and JA. Results indicated that overexpression and antisense expression of TaMPK4 in tobacco dramatically modify the growth of seedlings upon treatments of GA3, SA and JA, in which the overexpressors behaved deteriorated growth feature whereas the seedlings with antisense expression of TaMPK4 exhibited improved seedling phenotype. The growth behaviors in lines overexpressing or antisensely expressing TaMPK4 are closely associated with the biomass and the corresponding hormone-associated parameters. These results demonstrated that TaMPK4 acts as a critical player in mediating the phytohormone signaling. Our findings have identified the phytohormone-responsive MPK cascade genes in wheat and provided a connection between the phytohormone-mediated responses and the MPK cascade pathways.http://www.sciencedirect.com/science/article/pii/S2095311916613679wheat (Triticum aestivum L.)phytohormonemitogen-activated protein kinase (MPK) cascadeexpressiontransgene analysis |
| spellingShingle | Su-fei YAO Yan-xia WANG Tong-ren YANG Lin HAO Wen-jing LU Kai XIAO Expression and functional analyses of the mitogen-activated protein kinase (MPK) cascade genes in response to phytohormones in wheat (Triticum aestivum L.) Journal of Integrative Agriculture wheat (Triticum aestivum L.) phytohormone mitogen-activated protein kinase (MPK) cascade expression transgene analysis |
| title | Expression and functional analyses of the mitogen-activated protein kinase (MPK) cascade genes in response to phytohormones in wheat (Triticum aestivum L.) |
| title_full | Expression and functional analyses of the mitogen-activated protein kinase (MPK) cascade genes in response to phytohormones in wheat (Triticum aestivum L.) |
| title_fullStr | Expression and functional analyses of the mitogen-activated protein kinase (MPK) cascade genes in response to phytohormones in wheat (Triticum aestivum L.) |
| title_full_unstemmed | Expression and functional analyses of the mitogen-activated protein kinase (MPK) cascade genes in response to phytohormones in wheat (Triticum aestivum L.) |
| title_short | Expression and functional analyses of the mitogen-activated protein kinase (MPK) cascade genes in response to phytohormones in wheat (Triticum aestivum L.) |
| title_sort | expression and functional analyses of the mitogen activated protein kinase mpk cascade genes in response to phytohormones in wheat triticum aestivum l |
| topic | wheat (Triticum aestivum L.) phytohormone mitogen-activated protein kinase (MPK) cascade expression transgene analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2095311916613679 |
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