Chip-Oriented Fluorimeter Design and Detection System Development for DNA Quantification in Nano-Liter Volumes
The chip-based polymerase chain reaction (PCR) system has been developed in recent years to achieve DNA quantification. Using a microstructure and miniature chip, the volume consumption for a PCR can be reduced to a nano-liter. With high speed cycling and a low reaction volume, the time consumption...
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| Format: | Article |
| Language: | English |
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MDPI AG
2009-12-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/10/1/146/ |
| _version_ | 1828116691169574912 |
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| author | Da-Sheng Lee Ming-Hui Chen |
| author_facet | Da-Sheng Lee Ming-Hui Chen |
| author_sort | Da-Sheng Lee |
| collection | DOAJ |
| description | The chip-based polymerase chain reaction (PCR) system has been developed in recent years to achieve DNA quantification. Using a microstructure and miniature chip, the volume consumption for a PCR can be reduced to a nano-liter. With high speed cycling and a low reaction volume, the time consumption of one PCR cycle performed on a chip can be reduced. However, most of the presented prototypes employ commercial fluorimeters which are not optimized for fluorescence detection of such a small quantity sample. This limits the performance of DNA quantification, especially low experiment reproducibility. This study discusses the concept of a chip-oriented fluorimeter design. Using the analytical model, the current study analyzes the sensitivity and dynamic range of the fluorimeter to fit the requirements for detecting fluorescence in nano-liter volumes. Through the optimized processes, a real-time PCR on a chip system with only one nano-liter volume test sample is as sensitive as the commercial real-time PCR machine using the sample with twenty micro-liter volumes. The signal to noise (S/N) ratio of a chip system for DNA quantification with hepatitis B virus (HBV) plasmid samples is 3 dB higher. DNA quantification by the miniature chip shows higher reproducibility compared to the commercial machine with respect to samples of initial concentrations from 103 to 105 copies per reaction. |
| first_indexed | 2024-04-11T13:00:56Z |
| format | Article |
| id | doaj.art-d4b8a296d155425ea0f55cc959b38689 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T13:00:56Z |
| publishDate | 2009-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-d4b8a296d155425ea0f55cc959b386892022-12-22T04:22:56ZengMDPI AGSensors1424-82202009-12-0110114616610.3390/s100100146Chip-Oriented Fluorimeter Design and Detection System Development for DNA Quantification in Nano-Liter VolumesDa-Sheng LeeMing-Hui ChenThe chip-based polymerase chain reaction (PCR) system has been developed in recent years to achieve DNA quantification. Using a microstructure and miniature chip, the volume consumption for a PCR can be reduced to a nano-liter. With high speed cycling and a low reaction volume, the time consumption of one PCR cycle performed on a chip can be reduced. However, most of the presented prototypes employ commercial fluorimeters which are not optimized for fluorescence detection of such a small quantity sample. This limits the performance of DNA quantification, especially low experiment reproducibility. This study discusses the concept of a chip-oriented fluorimeter design. Using the analytical model, the current study analyzes the sensitivity and dynamic range of the fluorimeter to fit the requirements for detecting fluorescence in nano-liter volumes. Through the optimized processes, a real-time PCR on a chip system with only one nano-liter volume test sample is as sensitive as the commercial real-time PCR machine using the sample with twenty micro-liter volumes. The signal to noise (S/N) ratio of a chip system for DNA quantification with hepatitis B virus (HBV) plasmid samples is 3 dB higher. DNA quantification by the miniature chip shows higher reproducibility compared to the commercial machine with respect to samples of initial concentrations from 103 to 105 copies per reaction.http://www.mdpi.com/1424-8220/10/1/146/polymerase chain reaction (PCR)DNA quantificationchip-oriented fluorimeter designreal-time PCR on a chip systemS/N ratio |
| spellingShingle | Da-Sheng Lee Ming-Hui Chen Chip-Oriented Fluorimeter Design and Detection System Development for DNA Quantification in Nano-Liter Volumes Sensors polymerase chain reaction (PCR) DNA quantification chip-oriented fluorimeter design real-time PCR on a chip system S/N ratio |
| title | Chip-Oriented Fluorimeter Design and Detection System Development for DNA Quantification in Nano-Liter Volumes |
| title_full | Chip-Oriented Fluorimeter Design and Detection System Development for DNA Quantification in Nano-Liter Volumes |
| title_fullStr | Chip-Oriented Fluorimeter Design and Detection System Development for DNA Quantification in Nano-Liter Volumes |
| title_full_unstemmed | Chip-Oriented Fluorimeter Design and Detection System Development for DNA Quantification in Nano-Liter Volumes |
| title_short | Chip-Oriented Fluorimeter Design and Detection System Development for DNA Quantification in Nano-Liter Volumes |
| title_sort | chip oriented fluorimeter design and detection system development for dna quantification in nano liter volumes |
| topic | polymerase chain reaction (PCR) DNA quantification chip-oriented fluorimeter design real-time PCR on a chip system S/N ratio |
| url | http://www.mdpi.com/1424-8220/10/1/146/ |
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