Low Temperature Affects Fatty Acids Profiling and Key Synthesis Genes Expression Patterns in <i>Zanthoxylum bungeanum</i> Maxim
<i>Zanthoxylum bungeanum</i> is one of the most important medicinal and edible homologous plants because of its potential health benefits and unique flavors. The chemical components in compositions and contents vary with plant genotype variations and various environmental stress conditio...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/4/2319 |
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| author | Jieyun Tian Lu Tian Ming Chen Yabing Chen Anzhi Wei |
| author_facet | Jieyun Tian Lu Tian Ming Chen Yabing Chen Anzhi Wei |
| author_sort | Jieyun Tian |
| collection | DOAJ |
| description | <i>Zanthoxylum bungeanum</i> is one of the most important medicinal and edible homologous plants because of its potential health benefits and unique flavors. The chemical components in compositions and contents vary with plant genotype variations and various environmental stress conditions. Fatty acids participate in various important metabolic pathways in organisms to resist biotic and abiotic stresses. To determine the variations in metabolic profiling and genotypes, the fatty acid profiling and key differential genes under low temperature stress in two <i>Z. bungeanum</i> varieties, cold-tolerant (FG) and sensitive (FX), were investigated. Twelve main fatty acids were found in two <i>Z. bungeanum</i> varieties under cold stress. Results showed that the contents of total fatty acids and unsaturated fatty acids in FG were higher than those in FX, which made FG more resistant to low temperature. Based on the result of orthogonal partial least squares discriminant analysis, palmitic acid, isostearic acid, linolenic acid and eicosenoic acid were the important differential fatty acids in FG under cold stress, while isomyristic acid, palmitic acid, isostearic acid, stearic acid, oleic acid, linolenic acid and eicosenoic acid were the important differential fatty acids in FX. Furthermore, fatty acid synthesis pathway genes fatty acyl-ACP thioesterase A (<i>FATA</i>), Delta (8)-fatty-acid desaturase 2 (<i>SLD2</i>), protein ECERIFERUM 3 (<i>CER3</i>), fatty acid desaturase 3 (<i>FAD3</i>) and fatty acid desaturase 5 (<i>FAD5</i>) played key roles in FG, and <i>SLD2</i>, <i>FAD5</i>, 3-oxoacyl-[acyl-carrier-protein] synthase I (<i>KAS I</i>), fatty acyl-ACP thioesterase B (<i>FATB</i>) and acetyl-CoA carboxylase (<i>ACC</i>) were the key genes responding to low temperature in FX. The variation and strategies of fatty acids in two varieties of <i>Z. bungeanum</i> were revealed at the metabolic and molecular level. This work provides a reference for the study of chemical components in plant stress resistance. |
| first_indexed | 2024-03-09T21:44:27Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:44:27Z |
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| spelling | doaj.art-d4c291262ac44a15817be6faef2f0f722023-11-23T20:23:35ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01234231910.3390/ijms23042319Low Temperature Affects Fatty Acids Profiling and Key Synthesis Genes Expression Patterns in <i>Zanthoxylum bungeanum</i> MaximJieyun Tian0Lu Tian1Ming Chen2Yabing Chen3Anzhi Wei4College of Forestry, Northwest A&F University, Xianyang 712100, ChinaCollege of Forestry, Northwest A&F University, Xianyang 712100, ChinaCollege of Forestry, Northwest A&F University, Xianyang 712100, ChinaCollege of Forestry, Northwest A&F University, Xianyang 712100, ChinaCollege of Forestry, Northwest A&F University, Xianyang 712100, China<i>Zanthoxylum bungeanum</i> is one of the most important medicinal and edible homologous plants because of its potential health benefits and unique flavors. The chemical components in compositions and contents vary with plant genotype variations and various environmental stress conditions. Fatty acids participate in various important metabolic pathways in organisms to resist biotic and abiotic stresses. To determine the variations in metabolic profiling and genotypes, the fatty acid profiling and key differential genes under low temperature stress in two <i>Z. bungeanum</i> varieties, cold-tolerant (FG) and sensitive (FX), were investigated. Twelve main fatty acids were found in two <i>Z. bungeanum</i> varieties under cold stress. Results showed that the contents of total fatty acids and unsaturated fatty acids in FG were higher than those in FX, which made FG more resistant to low temperature. Based on the result of orthogonal partial least squares discriminant analysis, palmitic acid, isostearic acid, linolenic acid and eicosenoic acid were the important differential fatty acids in FG under cold stress, while isomyristic acid, palmitic acid, isostearic acid, stearic acid, oleic acid, linolenic acid and eicosenoic acid were the important differential fatty acids in FX. Furthermore, fatty acid synthesis pathway genes fatty acyl-ACP thioesterase A (<i>FATA</i>), Delta (8)-fatty-acid desaturase 2 (<i>SLD2</i>), protein ECERIFERUM 3 (<i>CER3</i>), fatty acid desaturase 3 (<i>FAD3</i>) and fatty acid desaturase 5 (<i>FAD5</i>) played key roles in FG, and <i>SLD2</i>, <i>FAD5</i>, 3-oxoacyl-[acyl-carrier-protein] synthase I (<i>KAS I</i>), fatty acyl-ACP thioesterase B (<i>FATB</i>) and acetyl-CoA carboxylase (<i>ACC</i>) were the key genes responding to low temperature in FX. The variation and strategies of fatty acids in two varieties of <i>Z. bungeanum</i> were revealed at the metabolic and molecular level. This work provides a reference for the study of chemical components in plant stress resistance.https://www.mdpi.com/1422-0067/23/4/2319<i>Zanthoxylum bungeanum</i>cold stressfatty acid profilingchemometrics analysiskey genes |
| spellingShingle | Jieyun Tian Lu Tian Ming Chen Yabing Chen Anzhi Wei Low Temperature Affects Fatty Acids Profiling and Key Synthesis Genes Expression Patterns in <i>Zanthoxylum bungeanum</i> Maxim International Journal of Molecular Sciences <i>Zanthoxylum bungeanum</i> cold stress fatty acid profiling chemometrics analysis key genes |
| title | Low Temperature Affects Fatty Acids Profiling and Key Synthesis Genes Expression Patterns in <i>Zanthoxylum bungeanum</i> Maxim |
| title_full | Low Temperature Affects Fatty Acids Profiling and Key Synthesis Genes Expression Patterns in <i>Zanthoxylum bungeanum</i> Maxim |
| title_fullStr | Low Temperature Affects Fatty Acids Profiling and Key Synthesis Genes Expression Patterns in <i>Zanthoxylum bungeanum</i> Maxim |
| title_full_unstemmed | Low Temperature Affects Fatty Acids Profiling and Key Synthesis Genes Expression Patterns in <i>Zanthoxylum bungeanum</i> Maxim |
| title_short | Low Temperature Affects Fatty Acids Profiling and Key Synthesis Genes Expression Patterns in <i>Zanthoxylum bungeanum</i> Maxim |
| title_sort | low temperature affects fatty acids profiling and key synthesis genes expression patterns in i zanthoxylum bungeanum i maxim |
| topic | <i>Zanthoxylum bungeanum</i> cold stress fatty acid profiling chemometrics analysis key genes |
| url | https://www.mdpi.com/1422-0067/23/4/2319 |
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