Nitrate-containing beetroot enhances myocyte metabolism and mitochondrial content
Beetroot (甜菜 tián cài) juice consumption is of current interest for improving aerobic performance by acting as a vasodilator and possibly through alterations in skeletal muscle metabolism and physiology. This work explored the effects of a commercially available beetroot supplement on metabolism, ge...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-01-01
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| Series: | Journal of Traditional and Complementary Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411014000479 |
| _version_ | 1818218308990664704 |
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| author | Roger A. Vaughan Nicholas P. Gannon Colin R. Carriker |
| author_facet | Roger A. Vaughan Nicholas P. Gannon Colin R. Carriker |
| author_sort | Roger A. Vaughan |
| collection | DOAJ |
| description | Beetroot (甜菜 tián cài) juice consumption is of current interest for improving aerobic performance by acting as a vasodilator and possibly through alterations in skeletal muscle metabolism and physiology. This work explored the effects of a commercially available beetroot supplement on metabolism, gene expression, and mitochondrial content in cultured myocytes. C2C12 myocytes were treated with various concentrations of the beetroot supplement for various durations. Glycolytic metabolism and oxidative metabolism were quantified via measurement of extracellular acidification and oxygen consumption, respectively. Metabolic gene expression was measured using quantitative reverse transcription–polymerase chain reaction, and mitochondrial content was assessed with flow cytometry and confocal microscopy. Cells treated with beetroot exhibited significantly increased oxidative metabolism, concurrently with elevated metabolic gene expression including peroxisome proliferator-activated receptor gamma coactivator-1 alpha, nuclear respiratory factor 1, mitochondrial transcription factor A, and glucose transporter 4, leading to increased mitochondrial biogenesis. Our data show that treatment with a beetroot supplement increases basal oxidative metabolism. Our observations are also among the first to demonstrate that beetroot extract is an inducer of metabolic gene expression and mitochondrial biogenesis. These observations support the need for further investigation into the therapeutic and pharmacological effects of nitrate-containing supplements for health and athletic benefits. |
| first_indexed | 2024-12-12T07:21:42Z |
| format | Article |
| id | doaj.art-d4ca10f1eeff4ac399a1ca58279c38fa |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-12-12T07:21:42Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-d4ca10f1eeff4ac399a1ca58279c38fa2022-12-22T00:33:20ZengElsevierJournal of Traditional and Complementary Medicine2225-41102016-01-0161172210.1016/j.jtcme.2014.11.033Nitrate-containing beetroot enhances myocyte metabolism and mitochondrial contentRoger A. Vaughan0Nicholas P. Gannon1Colin R. Carriker2Department of Nutritional Sciences, Texas Tech University, PO Box 41270, Lubbock 79409, TX, USADepartment of Health, Exercise and Sports Science, University of New Mexico, Albuquerque 87131, NM, USADepartment of Health, Exercise and Sports Science, University of New Mexico, Albuquerque 87131, NM, USABeetroot (甜菜 tián cài) juice consumption is of current interest for improving aerobic performance by acting as a vasodilator and possibly through alterations in skeletal muscle metabolism and physiology. This work explored the effects of a commercially available beetroot supplement on metabolism, gene expression, and mitochondrial content in cultured myocytes. C2C12 myocytes were treated with various concentrations of the beetroot supplement for various durations. Glycolytic metabolism and oxidative metabolism were quantified via measurement of extracellular acidification and oxygen consumption, respectively. Metabolic gene expression was measured using quantitative reverse transcription–polymerase chain reaction, and mitochondrial content was assessed with flow cytometry and confocal microscopy. Cells treated with beetroot exhibited significantly increased oxidative metabolism, concurrently with elevated metabolic gene expression including peroxisome proliferator-activated receptor gamma coactivator-1 alpha, nuclear respiratory factor 1, mitochondrial transcription factor A, and glucose transporter 4, leading to increased mitochondrial biogenesis. Our data show that treatment with a beetroot supplement increases basal oxidative metabolism. Our observations are also among the first to demonstrate that beetroot extract is an inducer of metabolic gene expression and mitochondrial biogenesis. These observations support the need for further investigation into the therapeutic and pharmacological effects of nitrate-containing supplements for health and athletic benefits.http://www.sciencedirect.com/science/article/pii/S2225411014000479dietary supplementergogenicmitochondrial uncoupling protein 3 (UCP3)nitratemitochondrial efficiencyperoxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) |
| spellingShingle | Roger A. Vaughan Nicholas P. Gannon Colin R. Carriker Nitrate-containing beetroot enhances myocyte metabolism and mitochondrial content Journal of Traditional and Complementary Medicine dietary supplement ergogenic mitochondrial uncoupling protein 3 (UCP3) nitrate mitochondrial efficiency peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) |
| title | Nitrate-containing beetroot enhances myocyte metabolism and mitochondrial content |
| title_full | Nitrate-containing beetroot enhances myocyte metabolism and mitochondrial content |
| title_fullStr | Nitrate-containing beetroot enhances myocyte metabolism and mitochondrial content |
| title_full_unstemmed | Nitrate-containing beetroot enhances myocyte metabolism and mitochondrial content |
| title_short | Nitrate-containing beetroot enhances myocyte metabolism and mitochondrial content |
| title_sort | nitrate containing beetroot enhances myocyte metabolism and mitochondrial content |
| topic | dietary supplement ergogenic mitochondrial uncoupling protein 3 (UCP3) nitrate mitochondrial efficiency peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) |
| url | http://www.sciencedirect.com/science/article/pii/S2225411014000479 |
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