The Healing Effects of Hydroalcoholic Extract of Carum Copticum L. on Experimental Colitis in Rats
Background: Ulcerative colitis is an inflammatory disease with indefinite treatment. The present study aimed to assess the anti-inflammatory and antioxidant effects of Carum copticum L. (CC) extract on induced colitis in rats. <br />Methods: Sixty male rats were randomly divided into six group...
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Shiraz University of Medical Sciences
2019-11-01
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| Series: | Iranian Journal of Medical Sciences |
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| Online Access: | http://ijms.sums.ac.ir/article_44961_cebf6b2a69c72e159bf38cbf9258b7f3.pdf |
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| author | Donya Firoozi Ali Akbar Nekooeian Nader Tanideh Zohreh Mazloom Maral Mokhtari Mohsen Mohammadi Sartang |
| author_facet | Donya Firoozi Ali Akbar Nekooeian Nader Tanideh Zohreh Mazloom Maral Mokhtari Mohsen Mohammadi Sartang |
| author_sort | Donya Firoozi |
| collection | DOAJ |
| description | Background: Ulcerative colitis is an inflammatory disease with indefinite treatment. The present study aimed to assess the anti-inflammatory and antioxidant effects of Carum copticum L. (CC) extract on induced colitis in rats. <br />Methods: Sixty male rats were randomly divided into six groups (n=10 per group). Acetic acid-induced colitis rats were orally administered with doses of 100, 200, and 400 mg/kg CC extract, and 100 mg/kg sulfasalazine for seven consecutive days, respectively. Colonic biopsies were taken to measure histopathological parameters as well as the tumor necrosis factor (TNF-α), interleukin-6 (IL-6), myeloperoxidase (MPO), malondialdehyde (MDA), and glutathione (GSH). Data analysis was performed using the one-way ANOVA and Tukey’s test for normally distributed data. Kruskal-Wallis test followed by Dunn’s test was used for non-normally distributed data. The analysis was performed at P≤0.05 using SigmaStat software (version 10.0).<br />Results: The control colitis group had a significantly higher total colitis index (P=0.01), TNF-α (P=0.01), IL-6 (P=0.01), MPO (P=0.01), and MDA (P=0.01); and lower GSH (P=0.01) than those of the sham group. The colitis group receiving a dose of 200 mg/kg/day CC extract had a significantly lower total colitis index (P=0.01), TNF-α (P=0.01), IL-6 (P=0.01), MPO (P=0.01), and MDA (P=0.01); and higher GSH (P=0.01) than those of the control colitis group. The colitis group receiving a dose of 200 mg/kg/day CC extract had a significantly lower total colitis index (P=0.04), TNF-α (P=0.03), IL-6 (P=0.04), MPO (P=0.03), and MDA (P=0.03); and higher GSH (P=0.01) than those of the colitis group receiving sulfasalazine. <br />Conclusion: The present study revealed that CC extract had healing effects on colitis, possibly due to its antioxidant and anti-inflammatory properties. |
| first_indexed | 2024-04-11T23:09:11Z |
| format | Article |
| id | doaj.art-d4cafa325b164bf09fe4230819d57f0a |
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| issn | 0253-0716 1735-3688 |
| language | English |
| last_indexed | 2024-04-11T23:09:11Z |
| publishDate | 2019-11-01 |
| publisher | Shiraz University of Medical Sciences |
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| series | Iranian Journal of Medical Sciences |
| spelling | doaj.art-d4cafa325b164bf09fe4230819d57f0a2022-12-22T03:57:55ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882019-11-0144650151010.30476/ijms.2019.4496144961The Healing Effects of Hydroalcoholic Extract of Carum Copticum L. on Experimental Colitis in RatsDonya Firoozi0Ali Akbar Nekooeian1Nader Tanideh2Zohreh Mazloom3Maral Mokhtari4Mohsen Mohammadi Sartang5Department of Nutrition and Diet Therapy, School of Nutrition and Food Science, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Clinical Nutrition, School of Nutrition and Food Science, Shiraz University of Medical Sciences, Shiraz, Iran;Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Nutrition, School of Nutrition and Food Science, Shiraz University of Medical Sciences, Shiraz, IranBackground: Ulcerative colitis is an inflammatory disease with indefinite treatment. The present study aimed to assess the anti-inflammatory and antioxidant effects of Carum copticum L. (CC) extract on induced colitis in rats. <br />Methods: Sixty male rats were randomly divided into six groups (n=10 per group). Acetic acid-induced colitis rats were orally administered with doses of 100, 200, and 400 mg/kg CC extract, and 100 mg/kg sulfasalazine for seven consecutive days, respectively. Colonic biopsies were taken to measure histopathological parameters as well as the tumor necrosis factor (TNF-α), interleukin-6 (IL-6), myeloperoxidase (MPO), malondialdehyde (MDA), and glutathione (GSH). Data analysis was performed using the one-way ANOVA and Tukey’s test for normally distributed data. Kruskal-Wallis test followed by Dunn’s test was used for non-normally distributed data. The analysis was performed at P≤0.05 using SigmaStat software (version 10.0).<br />Results: The control colitis group had a significantly higher total colitis index (P=0.01), TNF-α (P=0.01), IL-6 (P=0.01), MPO (P=0.01), and MDA (P=0.01); and lower GSH (P=0.01) than those of the sham group. The colitis group receiving a dose of 200 mg/kg/day CC extract had a significantly lower total colitis index (P=0.01), TNF-α (P=0.01), IL-6 (P=0.01), MPO (P=0.01), and MDA (P=0.01); and higher GSH (P=0.01) than those of the control colitis group. The colitis group receiving a dose of 200 mg/kg/day CC extract had a significantly lower total colitis index (P=0.04), TNF-α (P=0.03), IL-6 (P=0.04), MPO (P=0.03), and MDA (P=0.03); and higher GSH (P=0.01) than those of the colitis group receiving sulfasalazine. <br />Conclusion: The present study revealed that CC extract had healing effects on colitis, possibly due to its antioxidant and anti-inflammatory properties.http://ijms.sums.ac.ir/article_44961_cebf6b2a69c72e159bf38cbf9258b7f3.pdfcolitisulcerativeratscytokinesantioxidantsanti-inflammatory agents |
| spellingShingle | Donya Firoozi Ali Akbar Nekooeian Nader Tanideh Zohreh Mazloom Maral Mokhtari Mohsen Mohammadi Sartang The Healing Effects of Hydroalcoholic Extract of Carum Copticum L. on Experimental Colitis in Rats Iranian Journal of Medical Sciences colitis ulcerative rats cytokines antioxidants anti-inflammatory agents |
| title | The Healing Effects of Hydroalcoholic Extract of Carum Copticum L. on Experimental Colitis in Rats |
| title_full | The Healing Effects of Hydroalcoholic Extract of Carum Copticum L. on Experimental Colitis in Rats |
| title_fullStr | The Healing Effects of Hydroalcoholic Extract of Carum Copticum L. on Experimental Colitis in Rats |
| title_full_unstemmed | The Healing Effects of Hydroalcoholic Extract of Carum Copticum L. on Experimental Colitis in Rats |
| title_short | The Healing Effects of Hydroalcoholic Extract of Carum Copticum L. on Experimental Colitis in Rats |
| title_sort | healing effects of hydroalcoholic extract of carum copticum l on experimental colitis in rats |
| topic | colitis ulcerative rats cytokines antioxidants anti-inflammatory agents |
| url | http://ijms.sums.ac.ir/article_44961_cebf6b2a69c72e159bf38cbf9258b7f3.pdf |
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