Sero- and Molecular Epidemiology of HIV-1 in Papua Province, Indonesia
Background: human immunodeficiency virus (HIV) infection and acquired immune deficiency syndrome (AIDS) cause serious health problems and affect the Indonesian economy. Papua province has the highest prevalence of HIV infection in the country; however, epidemiological data are limited. Therefore, in...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Interna Publishing
2017-11-01
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| Series: | Acta Medica Indonesiana |
| Subjects: | |
| Online Access: | http://actamedindones.org/index.php/ijim/article/view/358 |
| _version_ | 1828472773279744000 |
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| author | Muhammad Qushai Yunifiar M Tomohiro Kotaki Adiana Mutamsari Witaningrum Siti Qamariyah Khairunisa Dwi Wahyu Indriati Meilani Meilani Tigor Yeheskiel Shuhei Ueda Nasronudin Nasronudin Masanori Kameoka |
| author_facet | Muhammad Qushai Yunifiar M Tomohiro Kotaki Adiana Mutamsari Witaningrum Siti Qamariyah Khairunisa Dwi Wahyu Indriati Meilani Meilani Tigor Yeheskiel Shuhei Ueda Nasronudin Nasronudin Masanori Kameoka |
| author_sort | Muhammad Qushai Yunifiar M |
| collection | DOAJ |
| description | Background: human immunodeficiency virus (HIV) infection and acquired immune deficiency syndrome (AIDS) cause serious health problems and affect the Indonesian economy. Papua province has the highest prevalence of HIV infection in the country; however, epidemiological data are limited. Therefore, in order to reveal the current situation of HIV/AIDS in Papua province, sero- and molecular epidemiological studies of HIV were conducted. Methods: serological tests were conducted on 157 healthy individuals from the general population residing in Paniai, Papua. In addition, a molecular epidemiological study was then conducted on HIV type 1 (HIV-1) genes derived from infected individuals. Peripheral blood samples from HIV-1-positive individuals and 15 additionally enrolled, previously confirmed HIV-1-positive individuals were subjected to a genotypic analysis. Results: serological tests revealed that 2 out of 157 (1.27%) healthy individuals were HIV-positive. In addition, HIV-1 subtyping revealed that subtype B and CRF01_AE were the major subtype and circulating recombinant form (CRF) of HIV-1 prevalent in the region, while subtype A1 and a recombinant form including viral gene fragments of CRF01_AE and subtype B was also detected. In addition, HIV drug resistance-associated major mutations were detected in the reverse transcriptase gene derived from infected individual on antiretroviral therapy. Conclusion: these results provide important information for clearer understanding on the current situation of HIV/AIDS in Papua province in Indonesia. |
| first_indexed | 2024-12-11T05:34:49Z |
| format | Article |
| id | doaj.art-d4cb07ee942e4e22bb391be110fa903e |
| institution | Directory Open Access Journal |
| issn | 0125-9326 2338-2732 |
| language | English |
| last_indexed | 2024-12-11T05:34:49Z |
| publishDate | 2017-11-01 |
| publisher | Interna Publishing |
| record_format | Article |
| series | Acta Medica Indonesiana |
| spelling | doaj.art-d4cb07ee942e4e22bb391be110fa903e2022-12-22T01:19:19ZengInterna PublishingActa Medica Indonesiana0125-93262338-27322017-11-01493219Sero- and Molecular Epidemiology of HIV-1 in Papua Province, IndonesiaMuhammad Qushai Yunifiar M0Tomohiro Kotaki1Adiana Mutamsari Witaningrum2Siti Qamariyah Khairunisa3Dwi Wahyu Indriati4Meilani Meilani5Tigor Yeheskiel6Shuhei Ueda7Nasronudin Nasronudin8Masanori Kameoka9Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Universitas Airlangga, Surabaya, IndonesiaDepartment of International Health, Kobe University Graduate School of Health Sciences, Hyogo, JapanIndonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Universitas Airlangga, Surabaya, IndonesiaIndonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Universitas Airlangga, Surabaya, IndonesiaIndonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia Department of Health, Vocational Faculty, Universitas Airlangga, Surabaya, IndonesiaDepartment of Health of Maluku Province, Maluku, IndonesiaTulehu General Hospital, Maluku, IndonesiaDepartment of International Health, Kobe University Graduate School of Health Sciences, Hyogo, JapanDepartment of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia. Airlangga Health Science Institute, Universitas Airlangga, Surabaya, IndonesiaDepartment of International Health, Kobe University Graduate School of Health Sciences, Hyogo, Japan. Center for Infectious Diseases, Kobe University Graduate School of Medicine, Hyogo, Japan.Background: human immunodeficiency virus (HIV) infection and acquired immune deficiency syndrome (AIDS) cause serious health problems and affect the Indonesian economy. Papua province has the highest prevalence of HIV infection in the country; however, epidemiological data are limited. Therefore, in order to reveal the current situation of HIV/AIDS in Papua province, sero- and molecular epidemiological studies of HIV were conducted. Methods: serological tests were conducted on 157 healthy individuals from the general population residing in Paniai, Papua. In addition, a molecular epidemiological study was then conducted on HIV type 1 (HIV-1) genes derived from infected individuals. Peripheral blood samples from HIV-1-positive individuals and 15 additionally enrolled, previously confirmed HIV-1-positive individuals were subjected to a genotypic analysis. Results: serological tests revealed that 2 out of 157 (1.27%) healthy individuals were HIV-positive. In addition, HIV-1 subtyping revealed that subtype B and CRF01_AE were the major subtype and circulating recombinant form (CRF) of HIV-1 prevalent in the region, while subtype A1 and a recombinant form including viral gene fragments of CRF01_AE and subtype B was also detected. In addition, HIV drug resistance-associated major mutations were detected in the reverse transcriptase gene derived from infected individual on antiretroviral therapy. Conclusion: these results provide important information for clearer understanding on the current situation of HIV/AIDS in Papua province in Indonesia.http://actamedindones.org/index.php/ijim/article/view/358HIV-1subtype BCRF01_AEHIV drug resistance |
| spellingShingle | Muhammad Qushai Yunifiar M Tomohiro Kotaki Adiana Mutamsari Witaningrum Siti Qamariyah Khairunisa Dwi Wahyu Indriati Meilani Meilani Tigor Yeheskiel Shuhei Ueda Nasronudin Nasronudin Masanori Kameoka Sero- and Molecular Epidemiology of HIV-1 in Papua Province, Indonesia Acta Medica Indonesiana HIV-1 subtype B CRF01_AE HIV drug resistance |
| title | Sero- and Molecular Epidemiology of HIV-1 in Papua Province, Indonesia |
| title_full | Sero- and Molecular Epidemiology of HIV-1 in Papua Province, Indonesia |
| title_fullStr | Sero- and Molecular Epidemiology of HIV-1 in Papua Province, Indonesia |
| title_full_unstemmed | Sero- and Molecular Epidemiology of HIV-1 in Papua Province, Indonesia |
| title_short | Sero- and Molecular Epidemiology of HIV-1 in Papua Province, Indonesia |
| title_sort | sero and molecular epidemiology of hiv 1 in papua province indonesia |
| topic | HIV-1 subtype B CRF01_AE HIV drug resistance |
| url | http://actamedindones.org/index.php/ijim/article/view/358 |
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