Extractive Fermentation as A Novel Strategy for High Cell Mass Production of Hetero-Fermentative Probiotic Strain <i>Limosilactobacillus reuteri</i>
This study reports on a novel technique to enhance the high cell mass and viable cell counts of the heterofermentative probiotic strain, <i>Limosilactobacillus reuteri</i>. This is the first report on the cultivation of <i>L. reuteri</i>, which was incorporated with weak base...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/10/527 |
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| author | Shanmugaprakasham Selvamani Solleh Ramli Daniel Joe Dailin Khairun Hani Natasya Theodoros Varzakas Bassam Abomoelak Dalia Sukmawati Muktiningsih Nurjayadi Siqing Liu Vijai Kumar Gupta Hesham Ali El Enshasy |
| author_facet | Shanmugaprakasham Selvamani Solleh Ramli Daniel Joe Dailin Khairun Hani Natasya Theodoros Varzakas Bassam Abomoelak Dalia Sukmawati Muktiningsih Nurjayadi Siqing Liu Vijai Kumar Gupta Hesham Ali El Enshasy |
| author_sort | Shanmugaprakasham Selvamani |
| collection | DOAJ |
| description | This study reports on a novel technique to enhance the high cell mass and viable cell counts of the heterofermentative probiotic strain, <i>Limosilactobacillus reuteri</i>. This is the first report on the cultivation of <i>L. reuteri</i>, which was incorporated with weak base anion-exchange resins to remove the accumulating lactic acid in the fermentation broth. Two anion-exchange resins—Amberlite IRA 67 and IRA 96—were found to have a high adsorption capacity with lactic acid. Batch fermentation and fed-batch cultivation were further analyzed using IRA 67 resins, as this application resulted in a higher maximum number of viable cells. The in situ application of anion-exchange resins was found to create shear stress, and thus, it does not promote growth of <i>L. reuteri</i>; therefore, an external and integrated resin column system was proposed. The viable cell count from batch fermentation, when incorporated with the integrated resin column, was improved by 71 times (3.89 × 10<sup>11</sup> ± 0.07 CFU mL<sup>−1</sup>) compared with control batch fermentation (5.35 × 10<sup>9</sup> ± 0.32 CFU mL<sup>−1</sup>), without the addition of resins. The growth improvement was achieved due to the high adsorption rate of lactic acid, which was recorded by the integrated IRA 67 resin system, and coupled with the stirred tank bioreactor batch fermentation process. |
| first_indexed | 2024-03-09T03:40:51Z |
| format | Article |
| id | doaj.art-d4ce35293efa4fedb4273398460cea15 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T03:40:51Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-d4ce35293efa4fedb4273398460cea152023-12-03T14:41:46ZengMDPI AGFermentation2311-56372022-10-0181052710.3390/fermentation8100527Extractive Fermentation as A Novel Strategy for High Cell Mass Production of Hetero-Fermentative Probiotic Strain <i>Limosilactobacillus reuteri</i>Shanmugaprakasham Selvamani0Solleh Ramli1Daniel Joe Dailin2Khairun Hani Natasya3Theodoros Varzakas4Bassam Abomoelak5Dalia Sukmawati6Muktiningsih Nurjayadi7Siqing Liu8Vijai Kumar Gupta9Hesham Ali El Enshasy10Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai 81310, MalaysiaInstitute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai 81310, MalaysiaInstitute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai 81310, MalaysiaInstitute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai 81310, MalaysiaInstitute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai 81310, MalaysiaArnold Palmer Hospital Paediatric Speciality Diagnostic Laboratory, Orlando, FL 32806, USAFaculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Jakarta Timur 13220, IndonesiaFaculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Jakarta Timur 13220, IndonesiaAgriculture Research Service, Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, USDA, 1815 N University St., Peoria, IL 61604, USABiorefining and Advanced Materials Research Center, SRUC, Kings Buildings, West Mains Road, Edinburgh EH9 3JG, UKInstitute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai 81310, MalaysiaThis study reports on a novel technique to enhance the high cell mass and viable cell counts of the heterofermentative probiotic strain, <i>Limosilactobacillus reuteri</i>. This is the first report on the cultivation of <i>L. reuteri</i>, which was incorporated with weak base anion-exchange resins to remove the accumulating lactic acid in the fermentation broth. Two anion-exchange resins—Amberlite IRA 67 and IRA 96—were found to have a high adsorption capacity with lactic acid. Batch fermentation and fed-batch cultivation were further analyzed using IRA 67 resins, as this application resulted in a higher maximum number of viable cells. The in situ application of anion-exchange resins was found to create shear stress, and thus, it does not promote growth of <i>L. reuteri</i>; therefore, an external and integrated resin column system was proposed. The viable cell count from batch fermentation, when incorporated with the integrated resin column, was improved by 71 times (3.89 × 10<sup>11</sup> ± 0.07 CFU mL<sup>−1</sup>) compared with control batch fermentation (5.35 × 10<sup>9</sup> ± 0.32 CFU mL<sup>−1</sup>), without the addition of resins. The growth improvement was achieved due to the high adsorption rate of lactic acid, which was recorded by the integrated IRA 67 resin system, and coupled with the stirred tank bioreactor batch fermentation process.https://www.mdpi.com/2311-5637/8/10/527lactic acid bacteriaheterofermentativehigh-cell densityextractive fermentationanion-exchange resinslactic acid removal |
| spellingShingle | Shanmugaprakasham Selvamani Solleh Ramli Daniel Joe Dailin Khairun Hani Natasya Theodoros Varzakas Bassam Abomoelak Dalia Sukmawati Muktiningsih Nurjayadi Siqing Liu Vijai Kumar Gupta Hesham Ali El Enshasy Extractive Fermentation as A Novel Strategy for High Cell Mass Production of Hetero-Fermentative Probiotic Strain <i>Limosilactobacillus reuteri</i> Fermentation lactic acid bacteria heterofermentative high-cell density extractive fermentation anion-exchange resins lactic acid removal |
| title | Extractive Fermentation as A Novel Strategy for High Cell Mass Production of Hetero-Fermentative Probiotic Strain <i>Limosilactobacillus reuteri</i> |
| title_full | Extractive Fermentation as A Novel Strategy for High Cell Mass Production of Hetero-Fermentative Probiotic Strain <i>Limosilactobacillus reuteri</i> |
| title_fullStr | Extractive Fermentation as A Novel Strategy for High Cell Mass Production of Hetero-Fermentative Probiotic Strain <i>Limosilactobacillus reuteri</i> |
| title_full_unstemmed | Extractive Fermentation as A Novel Strategy for High Cell Mass Production of Hetero-Fermentative Probiotic Strain <i>Limosilactobacillus reuteri</i> |
| title_short | Extractive Fermentation as A Novel Strategy for High Cell Mass Production of Hetero-Fermentative Probiotic Strain <i>Limosilactobacillus reuteri</i> |
| title_sort | extractive fermentation as a novel strategy for high cell mass production of hetero fermentative probiotic strain i limosilactobacillus reuteri i |
| topic | lactic acid bacteria heterofermentative high-cell density extractive fermentation anion-exchange resins lactic acid removal |
| url | https://www.mdpi.com/2311-5637/8/10/527 |
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