Comparative Transcriptome Analysis Reveals Novel Candidate Resistance Genes Involved in Defence against <i>Phytophthora cactorum</i> in Strawberry
Crown rot, caused by <i>Phytophthora cactorum</i>, is a devastating disease of strawberry. While most commercial octoploid strawberry cultivars (<i>Fragaria</i> × <i>ananassa</i> Duch) are generally susceptible, the diploid species <i>Fragaria vesca</i>...
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2023-06-01
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| author | Anupam Gogoi Erik Lysøe Håvard Eikemo Arne Stensvand Jahn Davik May Bente Brurberg |
| author_facet | Anupam Gogoi Erik Lysøe Håvard Eikemo Arne Stensvand Jahn Davik May Bente Brurberg |
| author_sort | Anupam Gogoi |
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| description | Crown rot, caused by <i>Phytophthora cactorum</i>, is a devastating disease of strawberry. While most commercial octoploid strawberry cultivars (<i>Fragaria</i> × <i>ananassa</i> Duch) are generally susceptible, the diploid species <i>Fragaria vesca</i> is a potential source of resistance genes to <i>P. cactorum</i>. We previously reported several <i>F. vesca</i> genotypes with varying degrees of resistance to <i>P. cactorum</i>. To gain insights into the strawberry defence mechanisms, comparative transcriptome profiles of two resistant genotypes (NCGR1603 and Bukammen) and a susceptible genotype (NCGR1218) of <i>F. vesca</i> were analysed by RNA-Seq after wounding and subsequent inoculation with <i>P. cactorum.</i> Differential gene expression analysis identified several defence-related genes that are highly expressed in the resistant genotypes relative to the susceptible genotype in response to <i>P. cactorum</i> after wounding. These included putative disease resistance (<i>R</i>) genes encoding receptor-like proteins, receptor-like kinases, nucleotide-binding sites, leucine-rich repeat proteins, RPW8-type disease resistance proteins, and ‘pathogenesis-related protein 1’. Seven of these <i>R</i>-genes were expressed only in the resistant genotypes and not in the susceptible genotype, and these appeared to be present only in the genomes of the resistant genotypes, as confirmed by PCR analysis. We previously reported a single major gene locus <i>RPc-1</i> (<i>Resistance to Phytophthora cactorum 1</i>) in <i>F. vesca</i> that contributed resistance to <i>P. cactorum</i>. Here, we report that 4–5% of the genes (35–38 of ca 800 genes) in the <i>RPc-1</i> locus are differentially expressed in the resistant genotypes compared to the susceptible genotype after inoculation with <i>P. cactorum</i>. In particular, we identified three defence-related genes encoding wall-associated receptor-like kinase 3, receptor-like protein 12, and non-specific lipid-transfer protein 1-like that were highly expressed in the resistant genotypes compared to the susceptible one. The present study reports several novel candidate disease resistance genes that warrant further investigation for their role in plant defence against <i>P. cactorum</i>. |
| first_indexed | 2024-03-11T01:39:27Z |
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| spelling | doaj.art-d4cf076ba5464a05a3e7eefc0d186ae12023-11-18T16:44:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124131085110.3390/ijms241310851Comparative Transcriptome Analysis Reveals Novel Candidate Resistance Genes Involved in Defence against <i>Phytophthora cactorum</i> in StrawberryAnupam Gogoi0Erik Lysøe1Håvard Eikemo2Arne Stensvand3Jahn Davik4May Bente Brurberg5Department of Plant Sciences, Faculty of Biosciences (BIOVIT), Norwegian University of Life Sciences (NMBU), 1433 Ås, NorwayDivision of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research (NIBIO), 1433 Ås, NorwayDivision of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research (NIBIO), 1433 Ås, NorwayDepartment of Plant Sciences, Faculty of Biosciences (BIOVIT), Norwegian University of Life Sciences (NMBU), 1433 Ås, NorwayDivision of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research (NIBIO), 1433 Ås, NorwayDepartment of Plant Sciences, Faculty of Biosciences (BIOVIT), Norwegian University of Life Sciences (NMBU), 1433 Ås, NorwayCrown rot, caused by <i>Phytophthora cactorum</i>, is a devastating disease of strawberry. While most commercial octoploid strawberry cultivars (<i>Fragaria</i> × <i>ananassa</i> Duch) are generally susceptible, the diploid species <i>Fragaria vesca</i> is a potential source of resistance genes to <i>P. cactorum</i>. We previously reported several <i>F. vesca</i> genotypes with varying degrees of resistance to <i>P. cactorum</i>. To gain insights into the strawberry defence mechanisms, comparative transcriptome profiles of two resistant genotypes (NCGR1603 and Bukammen) and a susceptible genotype (NCGR1218) of <i>F. vesca</i> were analysed by RNA-Seq after wounding and subsequent inoculation with <i>P. cactorum.</i> Differential gene expression analysis identified several defence-related genes that are highly expressed in the resistant genotypes relative to the susceptible genotype in response to <i>P. cactorum</i> after wounding. These included putative disease resistance (<i>R</i>) genes encoding receptor-like proteins, receptor-like kinases, nucleotide-binding sites, leucine-rich repeat proteins, RPW8-type disease resistance proteins, and ‘pathogenesis-related protein 1’. Seven of these <i>R</i>-genes were expressed only in the resistant genotypes and not in the susceptible genotype, and these appeared to be present only in the genomes of the resistant genotypes, as confirmed by PCR analysis. We previously reported a single major gene locus <i>RPc-1</i> (<i>Resistance to Phytophthora cactorum 1</i>) in <i>F. vesca</i> that contributed resistance to <i>P. cactorum</i>. Here, we report that 4–5% of the genes (35–38 of ca 800 genes) in the <i>RPc-1</i> locus are differentially expressed in the resistant genotypes compared to the susceptible genotype after inoculation with <i>P. cactorum</i>. In particular, we identified three defence-related genes encoding wall-associated receptor-like kinase 3, receptor-like protein 12, and non-specific lipid-transfer protein 1-like that were highly expressed in the resistant genotypes compared to the susceptible one. The present study reports several novel candidate disease resistance genes that warrant further investigation for their role in plant defence against <i>P. cactorum</i>.https://www.mdpi.com/1422-0067/24/13/10851crown rotdisease resistance (<i>R</i>-genes)<i>Fragaria vesca</i>oomycete |
| spellingShingle | Anupam Gogoi Erik Lysøe Håvard Eikemo Arne Stensvand Jahn Davik May Bente Brurberg Comparative Transcriptome Analysis Reveals Novel Candidate Resistance Genes Involved in Defence against <i>Phytophthora cactorum</i> in Strawberry International Journal of Molecular Sciences crown rot disease resistance (<i>R</i>-genes) <i>Fragaria vesca</i> oomycete |
| title | Comparative Transcriptome Analysis Reveals Novel Candidate Resistance Genes Involved in Defence against <i>Phytophthora cactorum</i> in Strawberry |
| title_full | Comparative Transcriptome Analysis Reveals Novel Candidate Resistance Genes Involved in Defence against <i>Phytophthora cactorum</i> in Strawberry |
| title_fullStr | Comparative Transcriptome Analysis Reveals Novel Candidate Resistance Genes Involved in Defence against <i>Phytophthora cactorum</i> in Strawberry |
| title_full_unstemmed | Comparative Transcriptome Analysis Reveals Novel Candidate Resistance Genes Involved in Defence against <i>Phytophthora cactorum</i> in Strawberry |
| title_short | Comparative Transcriptome Analysis Reveals Novel Candidate Resistance Genes Involved in Defence against <i>Phytophthora cactorum</i> in Strawberry |
| title_sort | comparative transcriptome analysis reveals novel candidate resistance genes involved in defence against i phytophthora cactorum i in strawberry |
| topic | crown rot disease resistance (<i>R</i>-genes) <i>Fragaria vesca</i> oomycete |
| url | https://www.mdpi.com/1422-0067/24/13/10851 |
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