Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In Vivo
Endometrial cancer (EC) is a very common female cancer which has attracted more and more attention. According to the individual patient’s condition, the current treatment of EC patients is mainly based on surgery, which is supplemented by chemotherapy, radiotherapy, and endocrine intervention. Howev...
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MDPI AG
2022-10-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/21/3415 |
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| author | Lei Ye Run-Hui Ma Xiu-Xiu Zhang Kiran Thakur Jian-Guo Zhang Mohammad Rizwan Khan Rosa Busquets Zhao-Jun Wei |
| author_facet | Lei Ye Run-Hui Ma Xiu-Xiu Zhang Kiran Thakur Jian-Guo Zhang Mohammad Rizwan Khan Rosa Busquets Zhao-Jun Wei |
| author_sort | Lei Ye |
| collection | DOAJ |
| description | Endometrial cancer (EC) is a very common female cancer which has attracted more and more attention. According to the individual patient’s condition, the current treatment of EC patients is mainly based on surgery, which is supplemented by chemotherapy, radiotherapy, and endocrine intervention. However, these existing treatment strategies also have some inevitable limitations. Therefore, it is particularly important to find an active ingredient with low toxicity and a high safety profile against EC. Isorhamnetin is a flavonoid known to be present in a variety of plants, such as sea buckthorn, dry willow, and wolfberry. In recent years, the anti-tumor effects of isorhamnetin have been reported. In our study, isorhamnetin was shown to induce apoptosis in Ishikawa cells by inducing the endogenous mitochondrial apoptotic pathway and exogenous death receptor pathway, promoting the endoplasmic reticulum stress-related pathway, and activating the corresponding markers of UPR response. In addition, isorhamnetin affected the expression of MMP2 and MMP9-related proteins in vitro and in vivo and eventually repressed metastasis. Therefore, isorhamnetin can be used as a promising medicinal material for the treatment of EC. |
| first_indexed | 2024-03-09T19:04:53Z |
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| id | doaj.art-d4d0c9ec0193432eb2c77aabce296939 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T19:04:53Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-d4d0c9ec0193432eb2c77aabce2969392023-11-24T04:40:26ZengMDPI AGFoods2304-81582022-10-011121341510.3390/foods11213415Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In VivoLei Ye0Run-Hui Ma1Xiu-Xiu Zhang2Kiran Thakur3Jian-Guo Zhang4Mohammad Rizwan Khan5Rosa Busquets6Zhao-Jun Wei7School of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaDepartment of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaSchool of Life Sciences, Pharmacy and Chemistry, Kingston University London, London KT1 2EE, UKSchool of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaEndometrial cancer (EC) is a very common female cancer which has attracted more and more attention. According to the individual patient’s condition, the current treatment of EC patients is mainly based on surgery, which is supplemented by chemotherapy, radiotherapy, and endocrine intervention. However, these existing treatment strategies also have some inevitable limitations. Therefore, it is particularly important to find an active ingredient with low toxicity and a high safety profile against EC. Isorhamnetin is a flavonoid known to be present in a variety of plants, such as sea buckthorn, dry willow, and wolfberry. In recent years, the anti-tumor effects of isorhamnetin have been reported. In our study, isorhamnetin was shown to induce apoptosis in Ishikawa cells by inducing the endogenous mitochondrial apoptotic pathway and exogenous death receptor pathway, promoting the endoplasmic reticulum stress-related pathway, and activating the corresponding markers of UPR response. In addition, isorhamnetin affected the expression of MMP2 and MMP9-related proteins in vitro and in vivo and eventually repressed metastasis. Therefore, isorhamnetin can be used as a promising medicinal material for the treatment of EC.https://www.mdpi.com/2304-8158/11/21/3415endometrial cancerIshikawaisorhamnetinUPR responsemitochondria |
| spellingShingle | Lei Ye Run-Hui Ma Xiu-Xiu Zhang Kiran Thakur Jian-Guo Zhang Mohammad Rizwan Khan Rosa Busquets Zhao-Jun Wei Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In Vivo Foods endometrial cancer Ishikawa isorhamnetin UPR response mitochondria |
| title | Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In Vivo |
| title_full | Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In Vivo |
| title_fullStr | Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In Vivo |
| title_full_unstemmed | Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In Vivo |
| title_short | Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In Vivo |
| title_sort | isorhamnetin induces apoptosis and suppresses metastasis of human endometrial carcinoma ishikawa cells via endoplasmic reticulum stress promotion and matrix metalloproteinase 2 9 inhibition in vitro and in vivo |
| topic | endometrial cancer Ishikawa isorhamnetin UPR response mitochondria |
| url | https://www.mdpi.com/2304-8158/11/21/3415 |
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