Feeding the Microbes: A Strategy to Control <i>Verticillium</i> Wilt
<i>Verticillium dahliae</i> is one of the most devastating soilborne pathogens for horticulture production. The pathogen has a broad host range and currently there is no effective chemical disease management, therefore, novel sustainable integrated disease management strategies should be...
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MDPI AG
2021-09-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/10/1946 |
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| author | Paraskevi Ziazia Eirini G. Poulaki Danai Gkizi Luis Lozano Mario Serrano Sotirios E. Tjamos |
| author_facet | Paraskevi Ziazia Eirini G. Poulaki Danai Gkizi Luis Lozano Mario Serrano Sotirios E. Tjamos |
| author_sort | Paraskevi Ziazia |
| collection | DOAJ |
| description | <i>Verticillium dahliae</i> is one of the most devastating soilborne pathogens for horticulture production. The pathogen has a broad host range and currently there is no effective chemical disease management, therefore, novel sustainable integrated disease management strategies should be considered. In this respect, we questioned whether the soil application of common microbiological growth media can influence the plant—microbe interactions and, subsequently, offer protection against <i>V. dahliae</i>. Indeed, the addition of Nutrient Broth (NB) and Potato Dextrose (PD) in non-sterilized soil reduced <i>Verticillium</i> wilt symptoms in eggplants. The addition of NB in sterilized soil did not reduce the disease symptoms compared to controls, however the addition of PD enhanced plant protection against <i>V. dahliae</i>. Following the results of a split root bioassay in eggplants, the possibility that NB and PD triggered the plant defense mechanisms against <i>V. dahliae</i> was excluded, since NB and PD did not reduce wilt symptom. Therefore, PD may be used as an easy food source for <i>V. dahliae,</i> detouring the pathogen from the root system of plants, while NB may affect the soil microbiome by enhancing antagonism in rhizosphere, or antagonistic interaction between <i>V. dahliae</i> and rhizospheric microbiome. Subsequently, several bacterial strains were isolated from the NB-treated rhizosphere and examined for their biocontrol activity against <i>V. dahliae</i>. Among the examined strains, a <i>Pseudomonas putida</i> strain, Z13, significantly reduced <i>Veticillium</i> severity and incidence under greenhouse conditions. |
| first_indexed | 2024-03-09T04:40:26Z |
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| id | doaj.art-d4d2e0fe5af34c74ac3389ad88946a38 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T04:40:26Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-d4d2e0fe5af34c74ac3389ad88946a382023-12-03T13:22:59ZengMDPI AGAgronomy2073-43952021-09-011110194610.3390/agronomy11101946Feeding the Microbes: A Strategy to Control <i>Verticillium</i> WiltParaskevi Ziazia0Eirini G. Poulaki1Danai Gkizi2Luis Lozano3Mario Serrano4Sotirios E. Tjamos5Laboratory of Phytopathology, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceLaboratory of Phytopathology, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceLaboratory of Phytopathology, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceCentro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad 2001, 62210 Cuernavaca, Morelos, MexicoCentro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad 2001, 62210 Cuernavaca, Morelos, MexicoLaboratory of Phytopathology, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece<i>Verticillium dahliae</i> is one of the most devastating soilborne pathogens for horticulture production. The pathogen has a broad host range and currently there is no effective chemical disease management, therefore, novel sustainable integrated disease management strategies should be considered. In this respect, we questioned whether the soil application of common microbiological growth media can influence the plant—microbe interactions and, subsequently, offer protection against <i>V. dahliae</i>. Indeed, the addition of Nutrient Broth (NB) and Potato Dextrose (PD) in non-sterilized soil reduced <i>Verticillium</i> wilt symptoms in eggplants. The addition of NB in sterilized soil did not reduce the disease symptoms compared to controls, however the addition of PD enhanced plant protection against <i>V. dahliae</i>. Following the results of a split root bioassay in eggplants, the possibility that NB and PD triggered the plant defense mechanisms against <i>V. dahliae</i> was excluded, since NB and PD did not reduce wilt symptom. Therefore, PD may be used as an easy food source for <i>V. dahliae,</i> detouring the pathogen from the root system of plants, while NB may affect the soil microbiome by enhancing antagonism in rhizosphere, or antagonistic interaction between <i>V. dahliae</i> and rhizospheric microbiome. Subsequently, several bacterial strains were isolated from the NB-treated rhizosphere and examined for their biocontrol activity against <i>V. dahliae</i>. Among the examined strains, a <i>Pseudomonas putida</i> strain, Z13, significantly reduced <i>Veticillium</i> severity and incidence under greenhouse conditions.https://www.mdpi.com/2073-4395/11/10/1946biological controlplant diseases<i>Pseudomonas putida</i>soilborne diseasesvascular wilts<i>Verticillium dahliae</i> |
| spellingShingle | Paraskevi Ziazia Eirini G. Poulaki Danai Gkizi Luis Lozano Mario Serrano Sotirios E. Tjamos Feeding the Microbes: A Strategy to Control <i>Verticillium</i> Wilt Agronomy biological control plant diseases <i>Pseudomonas putida</i> soilborne diseases vascular wilts <i>Verticillium dahliae</i> |
| title | Feeding the Microbes: A Strategy to Control <i>Verticillium</i> Wilt |
| title_full | Feeding the Microbes: A Strategy to Control <i>Verticillium</i> Wilt |
| title_fullStr | Feeding the Microbes: A Strategy to Control <i>Verticillium</i> Wilt |
| title_full_unstemmed | Feeding the Microbes: A Strategy to Control <i>Verticillium</i> Wilt |
| title_short | Feeding the Microbes: A Strategy to Control <i>Verticillium</i> Wilt |
| title_sort | feeding the microbes a strategy to control i verticillium i wilt |
| topic | biological control plant diseases <i>Pseudomonas putida</i> soilborne diseases vascular wilts <i>Verticillium dahliae</i> |
| url | https://www.mdpi.com/2073-4395/11/10/1946 |
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