Is leukotriene B4 one of the keloid marker? a fibroblast keloid study
Keloid pathogenesis occurs due to the duration of prolonged inflammatory phase and increased production of various growth factors such as TGF-β1 which may cause increasing fibroblast proliferation and collagen synthesis. Existence of one of the chemical mediators released during inflammation, leukot...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2019-03-01
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| Series: | Dermatology Reports |
| Subjects: | |
| Online Access: | https://www.pagepress.org/journals/index.php/dr/article/view/8045 |
| _version_ | 1818496335715762176 |
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| author | Yuli Kurniawati Oki Suwarsa A. Achadiyani Sudigdo Adi |
| author_facet | Yuli Kurniawati Oki Suwarsa A. Achadiyani Sudigdo Adi |
| author_sort | Yuli Kurniawati |
| collection | DOAJ |
| description | Keloid pathogenesis occurs due to the duration of prolonged inflammatory phase and increased production of various growth factors such as TGF-β1 which may cause increasing fibroblast proliferation and collagen synthesis. Existence of one of the chemical mediators released during inflammation, leukotriene B4 (LTB4), in keloid pathogenesis specifically in the phases of inflammation and proliferation, is still unclear. The purpose of the study is to analyze the levels of LTB4 in keloid. Methods: Fibroblast culture that was done by explanting keloid and normal skin of a keloid patient. Measurement of LTB4 on keloid and normal fibroblast was done by Elisa method. This experiment was run in triplicate. Statistical test was conducted by t test for the unpaired data and Anova test. The experiment was done at cell culture laboratory of Medical Faculty of Padjadjaran University Bandung. Levels of LTB4 in keloid fibroblast was higher than that of normal skin fibroblast (mean 23143.27 vs 18191.85 pg/ml; p<0.05). Conclusion, increased LTB4 levels in keloid fibroblast showed the existence of LTB4 role in the prolonged inflammatory process in keloid pathogenesis. |
| first_indexed | 2024-12-10T18:31:58Z |
| format | Article |
| id | doaj.art-d4d4e988d5844afc8fa0309457c13e60 |
| institution | Directory Open Access Journal |
| issn | 2036-7392 2036-7406 |
| language | English |
| last_indexed | 2024-12-10T18:31:58Z |
| publishDate | 2019-03-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Dermatology Reports |
| spelling | doaj.art-d4d4e988d5844afc8fa0309457c13e602022-12-22T01:37:55ZengPAGEPress PublicationsDermatology Reports2036-73922036-74062019-03-01111s10.4081/dr.2019.8045Is leukotriene B4 one of the keloid marker? a fibroblast keloid studyYuli Kurniawati0Oki Suwarsa1A. Achadiyani2Sudigdo Adi3Dermatology and Venereology Department, Faculty of Medicine, Sriwijaya University/RSUP Dr. Moh. Hoesin PalembangPostgraduate School of Medicine, Padjadjaran University, BandungPostgraduate School of Medicine, Padjadjaran University, BandungPostgraduate School of Medicine, Padjadjaran University, BandungKeloid pathogenesis occurs due to the duration of prolonged inflammatory phase and increased production of various growth factors such as TGF-β1 which may cause increasing fibroblast proliferation and collagen synthesis. Existence of one of the chemical mediators released during inflammation, leukotriene B4 (LTB4), in keloid pathogenesis specifically in the phases of inflammation and proliferation, is still unclear. The purpose of the study is to analyze the levels of LTB4 in keloid. Methods: Fibroblast culture that was done by explanting keloid and normal skin of a keloid patient. Measurement of LTB4 on keloid and normal fibroblast was done by Elisa method. This experiment was run in triplicate. Statistical test was conducted by t test for the unpaired data and Anova test. The experiment was done at cell culture laboratory of Medical Faculty of Padjadjaran University Bandung. Levels of LTB4 in keloid fibroblast was higher than that of normal skin fibroblast (mean 23143.27 vs 18191.85 pg/ml; p<0.05). Conclusion, increased LTB4 levels in keloid fibroblast showed the existence of LTB4 role in the prolonged inflammatory process in keloid pathogenesis.https://www.pagepress.org/journals/index.php/dr/article/view/8045fibroblastkeloidleukotriene B4 |
| spellingShingle | Yuli Kurniawati Oki Suwarsa A. Achadiyani Sudigdo Adi Is leukotriene B4 one of the keloid marker? a fibroblast keloid study Dermatology Reports fibroblast keloid leukotriene B4 |
| title | Is leukotriene B4 one of the keloid marker? a fibroblast keloid study |
| title_full | Is leukotriene B4 one of the keloid marker? a fibroblast keloid study |
| title_fullStr | Is leukotriene B4 one of the keloid marker? a fibroblast keloid study |
| title_full_unstemmed | Is leukotriene B4 one of the keloid marker? a fibroblast keloid study |
| title_short | Is leukotriene B4 one of the keloid marker? a fibroblast keloid study |
| title_sort | is leukotriene b4 one of the keloid marker a fibroblast keloid study |
| topic | fibroblast keloid leukotriene B4 |
| url | https://www.pagepress.org/journals/index.php/dr/article/view/8045 |
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