Development of Liquid-Phase Plasmonic Sensor Platforms for Prospective Biomedical Applications
Localized Surface Plasmon Resonance (LSPR) is an optical method for detecting changes in refractive index by the interaction between incident light and delocalized electrons within specific metal thin films’ localized “hot spots”. LSPR-based sensors possess advantages, including their compact size,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/24/1/186 |
| _version_ | 1797358154921541632 |
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| author | Sezin Sayin You Zhou Sheng Wang Andres Acosta Rodriguez Mona Zaghloul |
| author_facet | Sezin Sayin You Zhou Sheng Wang Andres Acosta Rodriguez Mona Zaghloul |
| author_sort | Sezin Sayin |
| collection | DOAJ |
| description | Localized Surface Plasmon Resonance (LSPR) is an optical method for detecting changes in refractive index by the interaction between incident light and delocalized electrons within specific metal thin films’ localized “hot spots”. LSPR-based sensors possess advantages, including their compact size, enhanced sensitivity, cost-effectiveness, and suitability for point-of-care applications. This research focuses on the development of LSPR-based nanohole arrays (NHAs) as a platform for monitoring probe/target binding events in real time without labeling, for low-level biomolecular target detection in biomedical diagnostics. To achieve this objective, this study involves creating a liquid-phase setup for capturing target molecules. Finite-difference time-domain simulations revealed that a 75 nm thickness of gold (Au) is ideal for NHA structures, which were visually examined using scanning electron microscopy. To illustrate the functionality of the liquid-phase sensor, a PDMS microfluidic channel was fabricated using a 3D-printed mold with a glass slide base and a top glass cover slip, enabling reflectance-mode measurements from each of four device sectors. This study shows the design, fabrication, and assessment of NHA-based LSPR sensor platforms within a PDMS microfluidic channel, confirming the sensor’s functionality and reproducibility in a liquid-phase environment. |
| first_indexed | 2024-03-08T14:57:46Z |
| format | Article |
| id | doaj.art-d4d716acd2ed49db9d548cc6851afc51 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-08T14:57:46Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-d4d716acd2ed49db9d548cc6851afc512024-01-10T15:08:57ZengMDPI AGSensors1424-82202023-12-0124118610.3390/s24010186Development of Liquid-Phase Plasmonic Sensor Platforms for Prospective Biomedical ApplicationsSezin Sayin0You Zhou1Sheng Wang2Andres Acosta Rodriguez3Mona Zaghloul4Department of Electrical and Computer Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC 20052, USADepartment of Electrical and Computer Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC 20052, USADepartment of Biomedical Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC 20052, USA3D Enviro, Barboursville, VA 22923, USADepartment of Electrical and Computer Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC 20052, USALocalized Surface Plasmon Resonance (LSPR) is an optical method for detecting changes in refractive index by the interaction between incident light and delocalized electrons within specific metal thin films’ localized “hot spots”. LSPR-based sensors possess advantages, including their compact size, enhanced sensitivity, cost-effectiveness, and suitability for point-of-care applications. This research focuses on the development of LSPR-based nanohole arrays (NHAs) as a platform for monitoring probe/target binding events in real time without labeling, for low-level biomolecular target detection in biomedical diagnostics. To achieve this objective, this study involves creating a liquid-phase setup for capturing target molecules. Finite-difference time-domain simulations revealed that a 75 nm thickness of gold (Au) is ideal for NHA structures, which were visually examined using scanning electron microscopy. To illustrate the functionality of the liquid-phase sensor, a PDMS microfluidic channel was fabricated using a 3D-printed mold with a glass slide base and a top glass cover slip, enabling reflectance-mode measurements from each of four device sectors. This study shows the design, fabrication, and assessment of NHA-based LSPR sensor platforms within a PDMS microfluidic channel, confirming the sensor’s functionality and reproducibility in a liquid-phase environment.https://www.mdpi.com/1424-8220/24/1/186localized surface plasmon resonancebiosensorplasmonic sensorbiomedical diagnosticsnanosensorsliquid-phase diagnostics |
| spellingShingle | Sezin Sayin You Zhou Sheng Wang Andres Acosta Rodriguez Mona Zaghloul Development of Liquid-Phase Plasmonic Sensor Platforms for Prospective Biomedical Applications Sensors localized surface plasmon resonance biosensor plasmonic sensor biomedical diagnostics nanosensors liquid-phase diagnostics |
| title | Development of Liquid-Phase Plasmonic Sensor Platforms for Prospective Biomedical Applications |
| title_full | Development of Liquid-Phase Plasmonic Sensor Platforms for Prospective Biomedical Applications |
| title_fullStr | Development of Liquid-Phase Plasmonic Sensor Platforms for Prospective Biomedical Applications |
| title_full_unstemmed | Development of Liquid-Phase Plasmonic Sensor Platforms for Prospective Biomedical Applications |
| title_short | Development of Liquid-Phase Plasmonic Sensor Platforms for Prospective Biomedical Applications |
| title_sort | development of liquid phase plasmonic sensor platforms for prospective biomedical applications |
| topic | localized surface plasmon resonance biosensor plasmonic sensor biomedical diagnostics nanosensors liquid-phase diagnostics |
| url | https://www.mdpi.com/1424-8220/24/1/186 |
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